Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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The Effects of Supplementation of BSA or Fatty Acid Free BAS on the Motility of Fresh or Cryopreserved Rooster Spermatozoa

BSA 또는 Fatty Acid Free BSA 첨가가 닭 희석 정자와 동결 정자의 생존성에 미치는 영향

  • Kim, Sung Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Min Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Yu, Yeonhui (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Chan-Lan (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Jeon, Ik Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Chongdae (Poultry Science Division, National Institute of Animal Science, RDA)
  • 김성우 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김민수 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 유연희 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김찬란 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 전익수 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김종대 (농촌진흥청 국립축산과학원 가금연구소)
  • Received : 2017.02.27
  • Accepted : 2017.03.29
  • Published : 2017.03.31
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Abstract

This study examined factors affecting the analysis of motility of chicken semen. The viability of spermatozoa was estimated using varying dilution ratios and supplementation with BSA or fatty acid free (FAF)-BSA as protein sources in semen diluent. Fresh semen was examined after preparing dilutions in beltsvile poultry semen extender (BPSE) of 1/8, 1/16 and 1/32 at $25^{\circ}C$. The motility of incubated semen at each dilution was observed at 3 min (89.9%, 69.9% and 53.2%), 30 min (86.7%, 71.4% and 51.7%), 1 h (89.5%, 74.0% and 53.5%) and 3 h (78.5%, 66.5% and 45.7%), respectively. The addition of BSA or FAF-BSA to BPSE diluent significantly increased the viability of semen in 1/32 dilution with results of 53.2% (control), 84.8% (BSA) and 92.9% (FAF-BSA) (p<0.05). This phenomenon was also observed in the dilution of frozen semen, where FAF-BSA treatment increased the viability of thawed semen from 17.6% to 34.0% in a 1/8 dilution (p<0.05). When the protein sources were used in the dilution, the survival rates of diluted chicken semen were also increased with time lapse. These results show that FAF-BSA may act to protect chicken semen and is suitable as a basic component of chicken semen diluent for the method of analyzing rooster semen after freezing.

본 연구에서는 닭 정액의 운동성 분석에 미치는 요인을 분석하기 위하여 희석배율에 따른 생존성을 조사하고, 신선정액의 희석 시에 필요한 조건을 확보하기 위하여 희석제에 단백질원으로서 BSA와 FAF-BSA의 첨가가 닭정자의 생존성에 미치는 영향을 관찰하였다. 최종 희석율이 1/4이 되도록 닭 정액을 희석하여 생존성을 조사하면 신선정액에서 희석율이 1/8, 1/16 및 1/32로 낮아질수록 89.9%, 69.9% 및 53.2%로 생존율이 낮아지는 것을 관찰하였으며, 단백질원으로 BSA나 FAF-BSA를 이용하면 생존율을 유의적으로 증진시킬 수 있음을 증명하였다. 또한 이러한 현상은 동결정액의 희석에도 중요한 요인으로 관찰되었는데, FAF-BSA를 이용하여 희석할 때 단백질이 없는 경우보다 양호한 생존율이 나타난다는 것을 관찰하였다(17.6% 대 34.0%). 그러나 단백질원을 활용하더라도 닭정액의 희석율이 낮아질수록 생존율이 저하하는 현상은 지속적으로 나타나고 있으며, 신선정액에 비하여 동결정액은 장수성이 낮은 것으로 추정되었다. 그러므로 본 연구에서 사용된 정자의 분석방법은 닭 정액동결을 실시하여 운동성을 분석할 때 필요한 기본 정보를 제공할 수 있을 것으로 판단되며, 닭 희석배율을 낮추어 동결정액의 효율성을 높이는 연구에 기여할 것으로 추정된다.

Keywords

References

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