Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Isolation of a zoonotic pathogen Aeromonas hydrophila from freshwater stingray (Potamotrygon motoro) kept in a Korean aquarium with ricefish (Oryzias latipes)

  • Yun, Saekil (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Lee, Young-Ran (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Giri, Sib Sankar (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Hyoun Joong (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Chi, Cheng (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Sang Guen (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Sang Wha (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Jun, Jin Woo (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Park, Se Chang (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • Received : 2016.11.16
  • Accepted : 2017.03.03
  • Published : 2017.03.31
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Abstract

In the present study, Aeromonas (A.) hydrophila was isolated from a captive-bred adult freshwater stingray (Potamotrygon motoro) reared at a commercial aquarium in Korea. The stingray had bites on its fins, hemorrhages on the ventral part, and congested internal organs. A bacterium was isolated from kidney and subsequently identified as A. hydrophila. Based on phylogenetic analysis results, the isolate in the present study (SNUAh-LA1) was most closely related to A. hydrophila AH10 (China) and A. hydrophila AKR1 (Korea). It is most likely that the pathogen infection resulted from Potamotrygon motoro cohabiting with ricefish (Oryzias latipes).

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References

  1. Allan BJ, Stevenson RM. Extracellular virulence factors of Aeromonas hydrophila in fish infections. Can J Microbiol 1981, 27, 1114-1122. https://doi.org/10.1139/m81-174
  2. Amin NED, Abdallah IS, Elallawy T, Ahmed SM. Motile Aeromonas septicaemia among Tilapia nilotica (Sarotherodon niloticus) in upper Egypt. Fish Pathol 1985, 20, 93-97. https://doi.org/10.3147/jsfp.20.93
  3. Austin B, Austin DA. Bacterial Fish Pathogens: Diseases in Farmed and Wild Fish. 2nd ed. pp. 111-117, Ellis Horwood, Chichester, 1993.
  4. Caras RA. The venomous fish. In: Venomous Animals of the World. pp. 103-116, Prentice-Hall, New York, 1974.
  5. CLSI. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; CLSI Approved Guideline M45-A. Clinical and Laboratory Standards Institute, Wayne, 2006.
  6. Harikrishnan R, Balasundaram C. Modern trends in Aeromonas hydrophila disease management with fish. Rev Fish Sci 2005, 13, 281-320. https://doi.org/10.1080/10641260500320845
  7. Janda JM, Abbott SL. Evolving concepts regarding the genus Aeromonas: an expanding panorama of species, disease presentations, and unanswered questions. Clin Infect Dis 1998, 27, 332-344. https://doi.org/10.1086/514652
  8. Ng HH, Tan HH, Yeo DCJ, Ng PKL. Stingers in a strange land: South American freshwater stingrays (Potamotrygonidae) in Singapore. Biol Invasions 2010, 12, 2385-2388. https://doi.org/10.1007/s10530-009-9663-5
  9. Persson S, Al-Shuweli S, Yapici S, Jensen JN, Olsen KEP. Identification of Clinical Aeromonas species by rpoB and gyrB sequencing and development of a multiplex PCR method for detection of Aeromonas hydrophila, A. caviae, A. veronii, and A. media. J Clin Microbiol 2015, 53, 653-656. https://doi.org/10.1128/JCM.01963-14
  10. Smith P, Hiney MP, Samuelsen OB. Bacterial resistance to antimicrobial agents used in fish farming: a critical evaluation of method and meaning. Annu Rev Fish Dis 1994, 4, 273-313. https://doi.org/10.1016/0959-8030(94)90032-9
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013, 30, 2725-9. https://doi.org/10.1093/molbev/mst197
  12. Tricas TC, Maruska KP, Rasmussen LEL. Annual cycles of steroid hormone production, gonad development, and reproductive behavior in the Atlantic stingray. Gen Comp Endocrinol 2000, 118, 209-225. https://doi.org/10.1006/gcen.2000.7466