Natural Product Sciences

  • 제23권1호
  • /
  • Pages.46-52
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  • 2017
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  • 1226-3907(pISSN)
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  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
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Comprehensive Evaluation of the Anti-Helicobacter pylori Activity of Scutellariae Radix

  • 투고 : 2016.11.22
  • 심사 : 2017.01.12
  • 발행 : 2017.03.31
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초록

The aim of this study was to evaluate the anti-Helicobacter pylori activity of fractions and major aglycon compounds (baicalein, chrysin, oroxylin A, wogonin) of Scutellariae Radix. Minimum inhibitory concentration (MIC) measurement, DPPH radical-scavenging assay, DNA protection assay, and urease inhibition analysis were performed. The ethyl acetate (EtOAc) fraction showed the potent anti-Helicobacter activity, and therefore, compounds in the EtOAc fraction were subjected to further assay. The MICs of chrysin, oroxylin A, and wogonin against Helicobacter pylori 26695 were 6.25, 12.5 and $25{\mu}g/mL$, respectively. Baicalein exhibited the most effective DPPH radical-scavenging activity. DNA protection using Fenton reaction, chrysin, oroxylin A, and wogonin showed effective DNA protective effect. This result was also confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Regarding Jack bean urease (0.5 mg/mL, 50 unit/mg) inhibition, 20 mM ofbaicalein and chrysin inhibited urease activity by 88.2% and 72.5%, respectively.

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참고문헌

  1. Peek, R. M.; Blaser, M. J. Nat. Rev. Cancer 2002, 2, 28-37. https://doi.org/10.1038/nrc703
  2. Kim, B. J.; Kim, J. G. Kor. J. Med. 2015, 89, 133-141. https://doi.org/10.3904/kjm.2015.89.2.133
  3. De Francesco, V. D.; Giorgio, F.; Hassan, C.; Manes, G.; Vannella, L.; Panella, C.; Ierardi, E.; Zullo, A. J. Gastrointestin. Liver Dis. 2010, 19, 409-414.
  4. Jung, K. W.; Won, Y. J.; Kong, H. J.; Oh, C. M.; Cho, H. S.; Lee, D.H.; Lee, K. H. Cancer Res. Treat. 2015, 47, 127-141. https://doi.org/10.4143/crt.2015.060
  5. Shin, A.; Park, S.; Shin, H. R.; Park, E. H.; Park, S. K.; Oh, J. K.; Lim, M. K.; Choi, B. Y.; Boniol, M.; Boffetta, P. Ann. Oncol. 2011, 22, 1435-1442. https://doi.org/10.1093/annonc/mdq592
  6. Shin, A. S.; Kim, J. S.; Park, S. H. J. Gastric Cancer 2011, 11, 135-140. https://doi.org/10.5230/jgc.2011.11.3.135
  7. Zhang, Q. B.; Nakshabendi, I. M.; Mokhashi, M. S.; Dawodu, J. B.; Gemmell, C. G.; Russell, R. I. Gut 1996, 38, 841-845. https://doi.org/10.1136/gut.38.6.841
  8. Shao, Z. H.; Li, C. Q.; Vanden Hoek, T.L.; Becker, L. B.; Schumacker, P. T.; Wu, J.A.; Attele, A.S.; Yuan, C. S. J. Mol. Cell. Cardiol. 1999, 31, 1885-1895. https://doi.org/10.1006/jmcc.1999.1021
  9. Huang, W. H.; Lee, A. R.; Yang, C. H. Biosci. Biotechnol. Biochem. 2006, 70, 2371-2380. https://doi.org/10.1271/bbb.50698
  10. Wang, Y. C.; Huang, K. M. Food Chem. Toxicol. 2013, 53, 376-383. https://doi.org/10.1016/j.fct.2012.12.018
  11. Ustun, O.; Ozcellik, B.; Akyon, Y.; Abbasoglu, U.; Yesilada, E. J. Ethnopharmacol. 2006, 108, 457-461. https://doi.org/10.1016/j.jep.2006.06.001
  12. Kang, M. H.; Lee, J. H.; Lee, Y. S.; Son, K. H.; Lee, D. H.; Kim, Y. S.; Kang, S. S.; Bang, H. C.; Jeong, C. S. Yakhak Hoeji 2007, 51,68-74.
  13. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing, 19th Informational Supplement. 2009. Document M100-S19, CLSI, Wayne, PA.
  14. Li, H. B.; Chen, F. J. Chromatogr. A. 2005, 1074, 107-110. https://doi.org/10.1016/j.chroma.2005.03.088
  15. Choi, J. S.; Oh, J. I.; Hwang, I. T.; Kim, S. E.; Chun, J. C.; Lee, B. H.; Kim, J. S.; Kim, T. J.; Cho, K. Y. Kor. J.Pestic. Sci. 2003, 7, 92-99.
  16. Lee, J. C.; Kim, H. R.; Kim, J.; Jang, Y. S. J. Agric. Food Chem. 2002, 50, 6490-6496. https://doi.org/10.1021/jf020388c
  17. Weatherburn, M. W. Anal. Chem. 1967, 39, 971-974. https://doi.org/10.1021/ac60252a045
  18. Choi, Y. S.; Cheon, J. H.; Lee, J. Y.; Kim, S. G.; Kim, J. S.; Kim, N. Y.; Lee, D. H.; Kim, J. M.; Jung, H. C.; Song, I. S. Korean J. Gastroenterol. 2006, 48,156-161.
  19. Wu, J.; Hu, D.; Wang, K. X. Zhong Yao Cai 2008, 31, 707-710.
  20. Shin, S. J.; Park, C. E.; Baek, N. I.; Chung, I. S.; Park, C. H. Biotechnol. Bioprocess Eng. 2009, 14, 140-145. https://doi.org/10.1007/s12257-008-0272-4
  21. Park, C. E.; Park, C. H. Korean Chem. Eng. Res. 2013, 51, 591-596. https://doi.org/10.9713/kcer.2013.51.5.591
  22. Tan, L.; Su, J.; Wu, D.; Yu, X.; Su, Z.; He, J.; Wu, X.; Kong, S.; Lai, X.; Lin, J.; Su, Z. Scientific World Journal. 2013, doi: 10.1155/2013/879501.
  23. Yu, X. D.; Zheng, R. B.; Xie, J. H.; Su, J. Y.; Huang, X. Q.; Wang, Y. H.; Zheng, Y. F.; Mo, Z. Z.; Wu, X. L.; Wu, D. W.; Liang, Y. E.; Zeng, H. F.; Su, Z. R.; Huang, P. J. Ethnopharmacol. 2015, 162, 69-78. https://doi.org/10.1016/j.jep.2014.12.041
  24. Wu, D. W.; Yu, X. D.; Xie, J. H.; Su, Z. Q.; Su, J. Y.; Tan, L. R.; Huang, X. Q.; Chen, J. N.; Su, Z. R. Fitoterapia. 2013, 91, 60-67. https://doi.org/10.1016/j.fitote.2013.08.012

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