Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Host Cell Nuclear Localization of Shigella flexneri Effector OspF Is Facilitated by SUMOylation

  • Jo, Kyungmin (Celltrion) ;
  • Kim, Eun Jin (Department of Pharmacy, College of Pharmacy, Hanyang University) ;
  • Yu, Hyun Jin (Department of Pharmacy, College of Pharmacy, Hanyang University) ;
  • Yun, Cheol-Heui (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Dong Wook (Department of Pharmacy, College of Pharmacy, Hanyang University)
  • Received : 2016.11.24
  • Accepted : 2016.12.19
  • Published : 2017.03.28
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Abstract

When Shigella infect host cells, various effecter molecules are delivered into the cytoplasm of the host cell through the type III secretion system (TTSS) to facilitate their invasion process and control the host immune responses. Among these effectors, the S. flexneri effector OspF dephosphorylates mitogen-activated protein kinases and translocates itself to the nucleus, thus preventing histone H3 modification to regulate expression of proinflammatory cytokines. Despite the critical role of OspF, the mechanism by which it localizes in the nucleus has remained to be elucidated. In the present study, we identified a potential small ubiquitin-related modifier (SUMO) modification site within OspF and we demonstrated that Shigella TTSS effector OspF is conjugated with SUMO in the host cell and this modification mediates the nuclear translocation of OspF. Our results show a bacterial virulence factor can exploit host post-translational machinery to execute its intracellular trafficking.

Keywords

References

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