Molecules and Cells

  • 제40권3호
  • /
  • Pages.195-201
  • /
  • 2017
  • /
  • 1016-8478(pISSN)
  • /
  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지
DOI QR코드

DOI QR Code

MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process

  • Dong, Ying (Department of Radiotherapy, The Tumor Hospital of Jilin Province) ;
  • Jin, Xintian (Department of Thoracic, The Tumor Hospital of Jilin Province) ;
  • Sun, Zhiqiang (Department of Invasive Technology, The Tumor Hospital of Jilin Province) ;
  • Zhao, Yueming (Department of Oncology, The Tumor Hospital of Jilin Province) ;
  • Song, Xianjing (Department of Cardiology, The Second Hospital of Jilin University)
  • 투고 : 2016.11.29
  • 심사 : 2017.02.08
  • 발행 : 2017.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3'UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability. Thus, miR-186 is a potential target for NSCLC therapy. miR-186 is proposed to be one of tumor-suppressors and may serve as a therapeutic target in NSCLC treatment.

키워드

참고문헌

  1. Bahri, S., Wang, S., Conder, R., Choy, J., Vlachos, S., Dong, K., Merino, C., Sigrist, S., Molnar, C., Yang, X., et al. (2010). The leading edge during dorsal closure as a model for epithelial plasticity: Pak is required for recruitment of the Scribble complex and septate junction formation. Development 137, 2023-2032. https://doi.org/10.1242/dev.045088
  2. Ben Halima, S., Siegel, G., and Rajendran, L. (2016). miR-186 in Alzheimer's disease: a big hope for a small RNA? J. Neurochem. 137, 308-311. https://doi.org/10.1111/jnc.13573
  3. Chou, J., Wang, B., Zheng, T., Li, X., Zheng, L., Hu, J., Zhang, Y., Xing, Y., and Xi, T. (2016). MALAT1 induced migration and invasion of human breast cancer cells by competitively binding miR-1 with cdc42. Biochem. Biophys. Res. Commun. 472, 262-269. https://doi.org/10.1016/j.bbrc.2016.02.102
  4. de Marinis, F., and Grossi, F. (2008). Clinical evidence for secondand third-line treatment options in advanced non-small cell lung cancer. Oncologist 13 Suppl 1, 14-20.
  5. Etienne-Manneville, S. (2004). Cdc42--the centre of polarity. J. Cell Sci. 117, 1291-1300. https://doi.org/10.1242/jcs.01115
  6. Hornberger, J., Hirsch, F.R., Li, Q., and Page, R.D. (2015). Outcome and economic implications of proteomic test-guided second- or thirdline treatment for advanced non-small cell lung cancer: extended analysis of the PROSE trial. Lung Cancer 88, 223-230. https://doi.org/10.1016/j.lungcan.2015.03.006
  7. Jemal, A., Center, M.M., DeSantis, C., and Ward, E.M. (2010). Global patterns of cancer incidence and mortality rates and trends. Cancer Epidemiol Biomarkers Prev. 19, 1893-1907. https://doi.org/10.1158/1055-9965.EPI-10-0437
  8. Ji, W., Sun, B., and Su, C. (2017). Targeting MicroRNAs in cancer gene therapy. Genes 8, pii: E21. https://doi.org/10.3390/genes8010021
  9. Jikuzono, T., Kawamoto, M., Yoshitake, H., Kikuchi, K., Akasu, H., Ishikawa, H., Hirokawa, M., Miyauchi, A., Tsuchiya, S., Shimizu, K., et al. (2013). The miR-221/222 cluster, miR-10b and miR-92a are highly upregulated in metastatic minimally invasive follicular thyroid carcinoma. Int. J. Oncol. 42, 1858-1868. https://doi.org/10.3892/ijo.2013.1879
  10. Lei, G.S., Kline, H.L., Lee, C.H., Wilkes, D.S., and Zhang, C. (2016). Regulation of collagen V expression and epithelial-mesenchymal transition by miR-185 and miR-186 during idiopathic pulmonary fibrosis. Am. J. Pathol. 186, 2310-2316. https://doi.org/10.1016/j.ajpath.2016.04.015
  11. Liu, L., Wang, Y., Bai, R., Yang, K., and Tian, Z. (2016a). MiR-186 inhibited aerobic glycolysis in gastric cancer via HIF-1${\alpha}$ regulation. Oncogenesis 5, e224. https://doi.org/10.1038/oncsis.2016.35
  12. Liu, Z., Zhang, G., Yu, W., Gao, N., and Peng, J. (2016b). miR-186 inhibits cell proliferation in multiple myeloma by repressing Jagged1. Biochem. Biophys. Res. Commun. 469, 692-697. https://doi.org/10.1016/j.bbrc.2015.11.136
  13. Profumo, V., and Gandellini, P. (2013). MicroRNAs: cobblestones on the road to cancer metastasis. Crit. Rev. Oncogenesis 18, 341-355. https://doi.org/10.1615/CritRevOncog.2013007182
  14. Ridley, A.J. (2015). Rho GTPase signalling in cell migration. Curr. Opin. Cell Bio. 36, 103-112. https://doi.org/10.1016/j.ceb.2015.08.005
  15. Sun, X.J., Liu, H., Zhang, P., Zhang, X.D., Jiang, Z.W., and Jiang, C.C. (2013). miR-10b promotes migration and invasion in nasopharyngeal carcinoma cells. Asian Pac. J. Cancer Prev. 14, 5533-5537. https://doi.org/10.7314/APJCP.2013.14.9.5533
  16. Terzuoli, E., Donnini, S., Finetti, F., Nesi, G., Villari, D., Hanaka, H., Radmark, O., Giachetti, A., and Ziche, M. (2016). Linking microsomal prostaglandin E Synthase-1/PGE-2 pathway with miR-15a and -186 expression: Novel mechanism of VEGF modulation in prostate cancer. Oncotarget 7, 44350-44364. https://doi.org/10.18632/oncotarget.10051
  17. Yao, Y., Zhang, X., Chen, H.P., Li, L., Xie, W., Lan, G., Zhao, Z.W., Zheng, X.L., Wang, Z.B., and Tang, C.K. (2016). MicroRNA-186 promotes macrophage lipid accumulation and secretion of proinflammatory cytokines by targeting cystathionine gamma-lyase in THP-1 macrophages. Atherosclerosis 250, 122-132. https://doi.org/10.1016/j.atherosclerosis.2016.04.030
  18. Yu, X., Zhang, X., Dhakal, I.B., Beggs, M., Kadlubar, S., and Luo, D. (2012). Induction of cell proliferation and survival genes by estradiolrepressed microRNAs in breast cancer cells. BMC Cancer 12, 29. https://doi.org/10.1186/1471-2407-12-29
  19. Zatulovskiy, E., Tyson, R., Bretschneider, T., and Kay, R.R. (2014). Bleb-driven chemotaxis of Dictyostelium cells. J. Cell Biol. 204, 1027-1044. https://doi.org/10.1083/jcb.201306147

피인용 문헌

  1. Suppression of Disheveled–Axin Domain Containing 1 (DIXDC1) by MicroRNA-186 Inhibits the Proliferation and Invasion of Retinoblastoma Cells vol.64, pp.2, 2018, https://doi.org/10.1007/s12031-017-1017-7
  2. MicroRNA in Lung Cancer Metastasis vol.11, pp.2, 2019, https://doi.org/10.3390/cancers11020265
  3. Bi-directional exosome-driven intercommunication between the hepatic niche and cancer cells vol.16, pp.1, 2017, https://doi.org/10.1186/s12943-017-0740-6
  4. miR-186, a serum microRNA, induces endothelial cell apoptosis by targeting SMAD6 in Kawasaki disease vol.41, pp.4, 2017, https://doi.org/10.3892/ijmm.2018.3397
  5. Regulating Cdc42 and Its Signaling Pathways in Cancer: Small Molecules and MicroRNA as New Treatment Candidates vol.23, pp.4, 2017, https://doi.org/10.3390/molecules23040787
  6. Micro-RNA-186-5p inhibition attenuates proliferation, anchorage independent growth and invasion in metastatic prostate cancer cells vol.18, pp.None, 2018, https://doi.org/10.1186/s12885-018-4258-0
  7. miR‐186 inhibits proliferation, migration, and epithelial‐mesenchymal transition in breast cancer cells by targeting Twist1 vol.120, pp.6, 2017, https://doi.org/10.1002/jcb.28283
  8. MORC2 promotes cell growth and metastasis in human cholangiocarcinoma and is negatively regulated by miR-186-5p vol.11, pp.11, 2017, https://doi.org/10.18632/aging.102003
  9. miR‐186 modulates hepatocellular carcinoma cell proliferation and mobility via targeting MCRS1‐mediated Wnt/β‐catenin signaling vol.234, pp.12, 2017, https://doi.org/10.1002/jcp.28878
  10. miR‐942 promotes tumor migration, invasion, and angiogenesis by regulating EMT via BARX2 in non‐small‐cell lung cancer vol.234, pp.12, 2017, https://doi.org/10.1002/jcp.28928
  11. Clinical Value of Serum and Exhaled Breath Condensate miR-186 and IL-1β Levels in Non-Small Cell Lung Cancer vol.19, pp.None, 2020, https://doi.org/10.1177/1533033820947490
  12. The effect of lncRNA‐ARAP1‐AS2/ARAP1 on high glucose‐induced cytoskeleton rearrangement and epithelial–mesenchymal transition in human renal tubular epithelial cells vol.235, pp.7, 2020, https://doi.org/10.1002/jcp.29512
  13. MicroRNA-629 promotes the tumorigenesis of non-small-cell lung cancer by targeting FOXO1 and activating PI3K/AKT pathway vol.29, pp.3, 2020, https://doi.org/10.3233/cbm-201685
  14. Regulators at Every Step—How microRNAs Drive Tumor Cell Invasiveness and Metastasis vol.12, pp.12, 2017, https://doi.org/10.3390/cancers12123709