The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Effects of acidic pH on voltage-gated ion channels in rat trigeminal mesencephalic nucleus neurons

  • Han, Jin-Eon (Department of Pharmacology, School of Dentistry, Kyungpook National University) ;
  • Cho, Jin-Hwa (Department of Pharmacology, School of Dentistry, Kyungpook National University) ;
  • Choi, In-Sun (Department of Pharmacology, School of Dentistry, Kyungpook National University) ;
  • Kim, Do-Yeon (Department of Pharmacology, School of Dentistry, Kyungpook National University) ;
  • Jang, Il-Sung (Department of Pharmacology, School of Dentistry, Kyungpook National University)
  • Received : 2016.11.08
  • Accepted : 2017.01.18
  • Published : 2017.03.01
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Abstract

The effects of acidic pH on several voltage-dependent ion channels, such as voltage-dependent $K^+$ and $Ca^{2+}$ channels, and hyperpolarization-gated and cyclic nucleotide-activated cation (HCN) channels, were examined using a whole-cell patch clamp technique on mechanically isolated rat mesencephalic trigeminal nucleus neurons. The application of a pH 6.5 solution had no effect on the peak amplitude of voltage-dependent $K^+$currents. A pH 6.0 solution slightly, but significantly inhibited the peak amplitude of voltage-dependent $K^+$ currents. The pH 6.0 also shifted both the current-voltage and conductance-voltage relationships to the depolarization range. The application of a pH 6.5 solution scarcely affected the peak amplitude of membrane currents mediated by HCN channels, which were profoundly inhibited by the general HCN channel blocker $Cs^+$ (1 mM). However, the pH 6.0 solution slightly, but significantly inhibited the peak amplitude of HCN-mediated currents. Although the pH 6.0 solution showed complex modulation of the current-voltage and conductance-voltage relationships, the midpoint voltages for the activation of HCN channels were not changed by acidic pH. On the other hand, voltage-dependent $Ca^{2+}$ channels were significantly inhibited by an acidic pH. The application of an acidic pH solution significantly shifted the current-voltage and conductance-voltage relationships to the depolarization range. The modulation of several voltage-dependent ion channels by an acidic pH might affect the excitability of mesencephalic trigeminal nucleus neurons, and thus physiological functions mediated by the mesencephalic trigeminal nucleus could be affected in acidic pH conditions.

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References

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