Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of DNA Markers for Fruit-related Traits and Genetic Relationships Based on Simple Sequence Repeat in Watermelon Accessions

  • Jin, Bingkui (Department of Horticultural Bioscience, Pusan National University) ;
  • Park, Girim (Department of Horticultural Bioscience, Pusan National University) ;
  • Choi, Youngmi (Department of Horticultural Bioscience, Pusan National University) ;
  • Nho, Jaejong (Jeollabuk-do Agricultural Research & Extension Services) ;
  • Son, Beunggu (Department of Horticultural Bioscience, Pusan National University) ;
  • Park, Younghoon (Department of Horticultural Bioscience, Pusan National University)
  • Received : 2016.06.15
  • Accepted : 2016.08.30
  • Published : 2017.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Modern watermelon cultivars (Citrullus lanatus [Thunb.] Matsum.& Nakai var. lanatus) have fruits with diverse phenotypes, including fruit shape, rind patterns, and flesh color. Molecular markers enable efficient selection of plants harboring desirable phenotypes. In the present study, publicly available DNA markers tightly linked to fruit shape, rind stripe pattern, and flesh color were evaluated using 85 watermelon accessions with diverse fruit phenotypes. For fruit shape, the dCAPS SUN - Cla011257 marker revealed an 81% of marker - trait match for accessions with elongated or round fruits. For rind stripe pattern, the SCAR wsb6-11marker was effective for selecting Jubilee-type rind pattern from other rind patterns. For flesh color, the Clcyb.600 and Lcyb markers derived from a mutation in the Lycopene ${\beta}$ - cyclase (Lcyb) gene, were effective at selecting red or yellow flesh. Forty-eight accessions possessing diverse fruit - related traits were selected as a reference array and their genetic relationships assessed using 16 SSR markers. At a coefficient of 0.11, the 48 accessions grouped into two major clades: Clade I and Clade II. Clade I subdivided further into subclades I - 1 and I - 2 at a coefficient of 0.39. All accessions with colored flesh were classified into Clade I, whereas those with white - flesh were classified into Clade II. Differences in fruit traits between subclades I - 1 and I - 2 were observed for rind pattern and fruit color; a majority of the accessions with Crimson-type striped or non-striped rind were grouped together in subclade I - 1, while most accessions in subclade I - 2 had a Jubilee - type rind stripe pattern. These results imply that reference array watermelon accessions possess distinguishable genetic structure based on rind stripe pattern. However, no significant grouping pattern was observed based on other fruit-related traits.

Keywords

References

  1. Bang HJ (2005) Environmental and genetic strategies to improve carotenoids and quality in watermelon. Texas A&M University. Ph.D. Thesis, Texas A&M University
  2. Bang H, Kim S, Leskovar D, King S (2007) Development of a codominant CAPS marker for allelic selection between canary yellow and red watermelon based on SNP in lycopene ${\beta}$-cyclase (LCYB) gene. Mol Breeding 20:63-72. doi:10.1007/s11032-006-9076-4
  3. Bang H, Yi G,Kim S (2014)Watermelon lycopene ${\beta}$-cyclase: promoter characterization leads to the development of a PCR marker for allelic selection. Euphytica 200:363-378. doi:10.1007/s10681-014-1158-5
  4. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314-331
  5. Devran Z, Goknur A, Mesci L (2016) Development of molecular markers for the Mi-1 gene in tomato using the KASP genotyping. Hortic Environ Biotechnol 57:156-160. doi:10.1007/s13580-016-0028-6
  6. Guner N, Wehner TC (2004) The genes of watermelon. HortScience 39:1175-1182
  7. Henderson WR (1989) Inheritance of orange flesh color in watermelon. Cucurbit Genetics Cooperative Report, 12: Article 26
  8. Henderson WR, Scott GH,Wehner TC (1998) Interaction of flesh color genes in watermelon. J Hered 89:50-53. doi:10.1093/jhered/89.1.50
  9. Isaacson T, Gil R, Dani Z, Joseph H (2002) Cloning of tangerine from tomato reveals a carotenoid isomerase essential for the production of ${\beta}$-carotene and xanthophylls in Plants. Plant Cell 14:333-342 https://doi.org/10.1105/tpc.010303
  10. Jeffery J, Davis A, King S (2012) Understanding the carotenoid biosynthesis pathway: observation of four color variants of development watermelon fruit. Israel J Plant Sci 60:425-434. doi:10.1105/tpc.010303.2001
  11. Kim KH, Ahn SG, Hwang JH,Chi YM, Mon HS,Pak YH (2013a) Inheritance of resistanceto powderymildew in the watermelon and developement of a molecular marker for selecting resistant plants. Hortic Environ Biotechnol 54:134-10. doi:10.1007/s13580-013-0156-1
  12. Kim H, Han D, Kang J, Choi Y, Levi A, Lee GP, Park Y (2015b) Sequence-characterized amplified polymorphism markers for selecting rind stripe pattern in watermelon (Citrullus lanatus L.). Hortic Environ Biotechnol 56:341-349. doi:10.1007/s13580-015-0017-1
  13. Kim KH, Hwang JH, Han DY, Park M, Kim S, Choi D,Park YH (2015c) Major quantitative trait loci and putative candidate genes for powdery mildew resistance and fruit-related traits revealed by an intraspecific genetic map for watermelon (Citrullus lanatus var. lanatus). PLos ONE 10(12):e0145665. doi:10.1371/journal.pone.0145665
  14. Kwon YS, Hong JH, Kim DH, Kim DH (2015) Use of microsatellite markers derived from genomic and expressed sequence tag (EST) data to identify commercial watermelon cultivars. Korean J Hortic Sci Technol 33:737-750. doi:10.7235/hort.2015.15045
  15. Li D, Cuevas HE, Yang L, Li Y, Garcia-Mas J, Zalapa J, Weng Y (2011) Syntenic relationships between cucumber (Cucumis sativus L.) and melon (C. melo L.) chromosomes as revealed by comparative genetic mapping. BMC Genomics 12:396-409. doi:10.1186/1471-2164-12-396
  16. Lou L (2009) Inheritance of fruit characteristics in watermelon [Citrullus lanatus (Thunb.) Matsum. &Nakai].Ph.D. Thesis, North Carolina State University
  17. Lv P, Li N, Liu H, Gu H, Zhao W (2015) Changes in carotenoid profiles and in the expression pattern of the genes in carotenoid metabolisms during fruit development and ripening in four watermelon cultivars. Food Chem 174:52-59. doi:10.1016/j.foodchem.2014.11.022
  18. Porter DR (1937) Inhertance of certain fruit and seed characters in watermelons. Hilgardia 10:489-509. doi:10.3733/hilg.v10n12p489
  19. Rhee S, Han B, Jang YJ, Sim TY, Lee GP (2015) Construction of a genetic linkage map using a frame set of simple sequence repeat and high-resolution melting markers for watermelon (Citrullus spp.). Hort Environ Biotechnol 56:669-676. doi:10.1007/s13580-015-0110-5
  20. Rohlf F (2002) NTSYS-pc: Numerical taxonomy system, version 2.1. Exeter Publishing. Ltd. Setauket, New York, USA
  21. Smith SM, Maughan PJ (2015) SNP genotyping using KASPar assays. In J Batley, ed, Plant Genotyping: Methods and Protocols. Ed 1, NY, Springer New York Heidelberg Dordrecht London, pp 243-256. doi:10.1007/978-1-4939-1966-6_18
  22. Tomes ML, Johnson KW (1965) Carotene pigments of an orange-fleshed watermelon. Proc Amer Soc Hort Sci 87:438-442
  23. Watanabe K, Saito T, Hirota S, Takahashi B (1987) Carotenoid pigments in red, orange and yellow fleshed fruits of watermelon (Citrullus vulgaris) cultivars. Engei Gakkai Zasshi 56:45-50. doi:10.2503/jjshs.56.45
  24. Weetman L (1937) Inheritance and correlation of shape, size and color in the watermelon, Citrullus vulgaris Schrad. Ia Agric Exp Sta 228:223-256