Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of lymphocyte DNA damage levels and total antioxidant capacity in Korean and American diet

  • Lee, Min Young (Department of Food Science and Nutrition, Daedeok Valley Campus, Hannam University) ;
  • Kim, Hyun A (Department of Food Science and Nutrition, Daedeok Valley Campus, Hannam University) ;
  • Kang, Myung Hee (Department of Food Science and Nutrition, Daedeok Valley Campus, Hannam University)
  • Received : 2016.11.04
  • Accepted : 2016.12.08
  • Published : 2017.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

BACKGROUND/OBJECTIVE: This study aims to measure the in vitro antioxidant capacity of Korean diet (KD) with American diet (AD) as a control group and to examine the ex vivo DNA damage reduction effect on human lymphocytes. MATERIALS/METHODS: The KD applied in this study is the standard one-week meals for Koreans (2,000 kcal/day) suggested by 2010 Dietary Reference Intakes for Koreans. The AD, which is the control group, is a one-week menu (2,000 kcal/day) that consists of foods that Americans would commonly take in according to the National Health and Nutrition Examination Survey. The antioxidant capacity of each menu was measured by means of the total phenolic assay and 3 in vitro antioxidant activity assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, trolox equivalent antioxidant capacity (TEAC), Oxygen radical absorbance capacity ($ORAC_{ROO{\cdot}}$)), while the extent of ex vivo lymphocyte DNA damage was measured by means of the comet assay. RESULTS: When measured by means of TEAC assay, the in vitro antioxidant capacity of the KD of the day was higher than that of the AD (P < 0.05) while there was no significant difference in total phenolic contents and DPPH and ORAC assays. The ex vivo lymphocyte DNA damage protective effect of the KD was significantly higher than that of the AD (P < 0.01). As for the one-week menu combining the menus for 7 days, the total phenolic assay (P < 0.05) and in vitro antioxidant capacity (P < 0.001, DPPH; P < 0.01, TEAC) of the KD menu were significantly higher than those of the AD menu. Likewise, the ex vivo DNA damage reduction rate of the Korean seven-day menu was significantly higher than that of the American menu (P < 0.01). CONCLUSION: This study demonstrates that the high antioxidant capacity and DNA damage protective effect of KD, which consists generally of various plant foods, are higher than those of typical AD.

Keywords

References

  1. Psaltopoulou T, Panagiotakos DB, Pitsavos C, Chrysochoou C, Detopoulou P, Skoumas J, Stefanadis C. Dietary antioxidant capacity is inversely associated with diabetes biomarkers: the ATTICA study. Nutr Metab Cardiovasc Dis 2011;21:561-7. https://doi.org/10.1016/j.numecd.2009.11.005
  2. Suhail N, Bilal N, Khan HY, Hasan S, Sharma S, Khan F, Mansoor T, Banu N. Effect of vitamins C and E on antioxidant status of breast-cancer patients undergoing chemotherapy. J Clin Pharm Ther 2012;37:22-6. https://doi.org/10.1111/j.1365-2710.2010.01237.x
  3. Cocate PG, Natali AJ, Alfenas RC, de Oliveira A, dos Santos EC, Hermsdorff HH. Carotenoid consumption is related to lower lipid oxidation and DNA damage in middle-aged men. Br J Nutr 2015;114:257-64. https://doi.org/10.1017/S0007114515001622
  4. Strain JJ, Elwood PC, Davis A, Kennedy O, Coulter J, Fehily A, Mulholland CW, Robson PJ, Thurnham DI. Frequency of fruit and vegetable consumption and blood antioxidants in the Caerphilly cohort of older men. Eur J Clin Nutr 2000;54:828-33. https://doi.org/10.1038/sj.ejcn.1601101
  5. Liu S, Lee IM, Ajani U, Cole SR, Buring JE, Manson JE; Physicians' Health Study. Intake of vegetables rich in carotenoids and risk of coronary heart disease in men: the Physicians' Health Study. Int J Epidemiol 2001;30:130-5. https://doi.org/10.1093/ije/30.1.130
  6. Hung HC, Joshipura KJ, Jiang R, Hu FB, Hunter D, Smith-Warner SA, Colditz GA, Rosner B, Spiegelman D, Willett WC. Fruit and vegetable intake and risk of major chronic disease. J Natl Cancer Inst 2004;96:1577-84. https://doi.org/10.1093/jnci/djh296
  7. Gaziano JM, Hennekens CH. The role of beta-carotene in the prevention of cardiovascular disease. Ann N Y Acad Sci 1993;691:148-55. https://doi.org/10.1111/j.1749-6632.1993.tb26166.x
  8. Kim SH, Kim MS, Lee MS, Park YS, Lee HJ, Kang SA, Lee HS, Lee KE, Yang HJ, Kim MJ, Lee YE, Kwon DY. Korean diet: characteristics and historical background. J Ethn Food 2016;3:26-31. https://doi.org/10.1016/j.jef.2016.03.002
  9. Kim S, Moon S, Popkin BM. The nutrition transition in South Korea. Am J Clin Nutr 2000;71:44-53. https://doi.org/10.1093/ajcn/71.1.44
  10. Lee MJ, Popkin BM, Kim S. The unique aspects of the nutrition transition in South Korea: the retention of healthful elements in their traditional diet. Public Health Nutr 2002;5:197-203.
  11. Song Y, Joung H. A traditional Korean dietary pattern and metabolic syndrome abnormalities. Nutr Metab Cardiovasc Dis 2012;22:456-62. https://doi.org/10.1016/j.numecd.2010.09.002
  12. Jung SJ, Park SH, Choi EK, Cha YS, Cho BH, Kim YG, Kim MG, Song WO, Park TS, Ko JK, So BO, Chae SW. Beneficial effects of Korean traditional diets in hypertensive and type 2 diabetic patients. J Med Food 2014;17:161-71. https://doi.org/10.1089/jmf.2013.3042
  13. Schroeder N, Park YH, Kang MS, Kim Y, Ha GK, Kim HR, Yates AA, Caballero B. A randomized trial on the effects of 2010 Dietary Guidelines for Americans and Korean diet patterns on cardiovascular risk factors in overweight and obese adults. J Acad Nutr Diet 2015;115:1083-92. https://doi.org/10.1016/j.jand.2015.03.023
  14. Shin JY, Kim JM, Kim Y. Associations between dietary patterns and hypertension among Korean adults: the Korean National Health and Nutrition Examination Survey (2008-2010). Nutr Res Pract 2013;7:224-32. https://doi.org/10.4162/nrp.2013.7.3.224
  15. Choi JH, Woo HD, Lee JH, Kim J. Dietary patterns and risk for metabolic syndrome in Korean women: a cross-sectional study. Medicine (Baltimore) 2015;94:e1424. https://doi.org/10.1097/MD.0000000000001424
  16. Saura-Calixto F, Goni I. Antioxidant capacity of the Spanish Mediterranean diet. Food Chem 2006;94:442-7. https://doi.org/10.1016/j.foodchem.2004.11.033
  17. Park Y, Lee J, Oh JH, Shin A, Kim J. Dietary patterns and colorectal cancer risk in a Korean population: a case-control study. Medicine (Baltimore) 2016;95:e3759. https://doi.org/10.1097/MD.0000000000003759
  18. Serafini M, Del Rio D. Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity the right tool? Redox Rep 2004;9:145-52. https://doi.org/10.1179/135100004225004814
  19. Suh JH, Paek OJ, Kang YW, Ahn JE, Yun JS, Oh KS, An YS, Park SH, Lee SJ. Study on the antioxidant activity in the various vegetables. J Food Hyg Saf 2013;28:337-41. https://doi.org/10.13103/JFHS.2013.28.4.337
  20. Yang M, Chung SJ, Chung CE, Kim DO, Song WO, Koo SI, Chun OK. Estimation of total antioxidant capacity from diet and supplements in US adults. Br J Nutr 2011;106:254-63. https://doi.org/10.1017/S0007114511000109
  21. Russnes KM, Moller E, Wilson KM, Carlsen M, Blomhoff R, Smeland S, Adami HO, Gronberg H, Mucci LA, Balter K. Total antioxidant intake and prostate cancer in the Cancer of the Prostate in Sweden (CAPS) study. A case control study. BMC Cancer 2016;16:438. https://doi.org/10.1186/s12885-016-2486-8
  22. Han JH, Lee HJ, Cho MR, Chang N, Kim Y, Oh SY, Kang MH. Total antioxidant capacity of the Korean diet. Nutr Res Pract 2014;8:183-91. https://doi.org/10.4162/nrp.2014.8.2.183
  23. Park YK, Jeon EJ, Kang MH. Protective effect of flavonoids on lymphocyte DNA damage using comet assay. Korean J Nutr 2003;36:125-32.
  24. Gafrikova M, Galova E, Sevcovicova A, Imreova P, Mucaji P, Miadokova E. Extract from Armoracia rusticana and its flavonoid components protect human lymphocytes against oxidative damage induced by hydrogen peroxide. Molecules 2014;19:3160-72. https://doi.org/10.3390/molecules19033160
  25. Jayakumar R, Kanthimathi MS. Dietary spices protect against hydrogen peroxide-induced DNA damage and inhibit nicotineinduced cancer cell migration. Food Chem 2012;134:1580-4. https://doi.org/10.1016/j.foodchem.2012.03.101
  26. Mullner E, Brath H, Pleifer S, Schiermayr C, Baierl A, Wallner M, Fastian T, Millner Y, Paller K, Henriksen T, Poulsen HE, Forster E, Wagner KH. Vegetables and PUFA-rich plant oil reduce DNA strand breaks in individuals with type 2 diabetes. Mol Nutr Food Res 2013;57:328-38. https://doi.org/10.1002/mnfr.201200343
  27. Prado RP, dos Santos BF, Pinto CL, de Assis KR, Salvadori DM, Ladeira MS. Influence of diet on oxidative DNA damage, uracil misincorporation and DNA repair capability. Mutagenesis 2010;25:483-7. https://doi.org/10.1093/mutage/geq030
  28. Switzeny OJ, Mullner E, Wagner KH, Brath H, Aumuller E, Haslberger AG. Vitamin and antioxidant rich diet increases MLH1 promoter DNA methylation in DMT2 subjects. Clin Epigenetics 2012;4:19. https://doi.org/10.1186/1868-7083-4-19
  29. The Korean Nutrition Society. Dietary Referece Intakes for Koreans. 1st rev. ed. Seoul: The Korean Nutrition Society; 2010. p.614.
  30. Korean Society of Food Science and Technology. Encyclopedia of Food Science and Technology. Seoul: Kwangil Publishing Co.; 2004.
  31. Randhir R, Shetty P, Shetty K. L-DOPA and total phenolic stimulation in dark germinated fava bean in response to peptide and phytochemical elicitors. Process Biochem 2002;37:1247-56. https://doi.org/10.1016/S0032-9592(02)00006-7
  32. Chen HM, Muramoto K, Yamauchi F, Fujimoto K, Nokihara K. Antioxidative properties of histidine-containing peptides designed from peptide fragments found in the digests of a soybean protein. J Agric Food Chem 1998;46:49-53. https://doi.org/10.1021/jf970649w
  33. Shon MY. Synergistic effect of methanol extract from kalopanax pictus and ascorbic acid on antioxidant, anticancer and immunomodulatory activities. J Life Sci 2007;17:1634-40. https://doi.org/10.5352/JLS.2007.17.12.1634
  34. Kurihara H, Fukami H, Asami S, Toyoda Y, Nakai M, Shibata H, Yao XS. Effects of oolong tea on plasma antioxidative capacity in mice loaded with restraint stress assessed using the oxygen radical absorbance capacity (ORAC) assay. Biol Pharm Bull 2004;27:1093-8. https://doi.org/10.1248/bpb.27.1093
  35. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184-91. https://doi.org/10.1016/0014-4827(88)90265-0
  36. Park YK, Kim JS, Jeon EJ, Kang MH. The improvement of chaga mushroom (Inonotus Obliquus) extract supplementation on the blood glucose and cellular DNA damage in streptozotocin-induced diabetic rats. Korean J Nutr 2009;42:5-13.
  37. Lee KW, Cho MS. The development and validation of the Korean dietary pattern score (KDPS). Korean J Food Cult 2010;25:652-60.
  38. Zamora-Ros R, Serafini M, Estruch R, Lamuela-Raventos RM, Martinez-Gonzalez MA, Salas-Salvado J, Fiol M, Lapetra J, Aros F, Covas MI, Andres-Lacueva C; PREDIMED Study Investigators. Mediterranean diet and non enzymatic antioxidant capacity in the PREDIMED study: evidence for a mechanism of antioxidant tuning. Nutr Metab Cardiovasc Dis 2013;23:1167-74. https://doi.org/10.1016/j.numecd.2012.12.008
  39. Rockett JC, Burczynski ME, Fornace AJ Jr, Herrmann PC, Krawetz SA, Dix DJ. Surrogate tissue analysis: monitoring toxicant exposure and health status of inaccessible tissues through the analysis of accessible tissues and cells. Toxicol Appl Pharmacol 2004;194:189-99. https://doi.org/10.1016/j.taap.2003.09.005

Cited by

  1. DNA strand breaks in peripheral blood leucocytes of Polish blood donors pp.1464-3804, 2017, https://doi.org/10.1093/mutage/gex024
  2. 건강한 성인의 glutathione S-transferase M1과 T1 유전자 다형성에 따른 한식에서의 식물성 식품군과 한식의 DNA 손상 감소 효과 vol.50, pp.1, 2017, https://doi.org/10.4163/jnh.2017.50.1.10