Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Development of a single-nucleotide-polymorphism marker for specific authentication of Korean ginseng (Panax ginseng Meyer) new cultivar "G-1"

  • Yang, Dong-Uk (Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University) ;
  • Kim, Min-Kyeoung (KM Fundamental Research Division, Korea Institute of Oriental Medicine) ;
  • Mohanan, Padmanaban (Graduate School of Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University) ;
  • Mathiyalagan, Ramya (Graduate School of Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University) ;
  • Seo, Kwang-Hoon (Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University) ;
  • Kwon, Woo-Saeng (Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University) ;
  • Yang, Deok-Chun (Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University)
  • Received : 2015.03.10
  • Accepted : 2015.12.15
  • Published : 2017.01.15
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Abstract

Background: Korean ginseng (Panax ginseng) is a well-known medicinal plant of Oriental medicine that is still in practice today. Until now, a total of 11 Korean ginseng cultivars with unique features to Korean ginseng have been developed based on the pure-line-selection method. Among them, a new cultivar namely G-1 with different agricultural traits related to yield and content of ginsenosides, was developed in 2012. Methods: The aim of this study was to distinguish the new ginseng cultivar G-1 by identifying the unique single-nucleotide polymorphism (SNP) at its 45S ribosomal DNA and Panax quinquefolius region than other Korean ginseng cultivars using multiplex amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR). Results: A SNP at position of 45S ribosomal DNA region between G-1, P. quinquefolius, and the other Korean ginseng cultivars was identified. By designing modified allele-specific primers based on this site, we could specifically identified G-1 and P. quinquefolius via multiplex PCR. The unique primer for the SNP yielded an amplicon of size 449 bp in G-1 cultivar and P. quinquefolius. This study presents an effective method for the genetic identification of the G-1 cultivar and P. quinquefolius. Conclusion: The results from our study shows that this SNP-based approach to identify the G-1 cultivar will be a good way to distinguish accurately the G-1 cultivar and P. quinquefolius from other Korean ginseng cultivars using a SNP at 45S ribosomal DNA region.

Keywords

References

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