The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of SaengRyo-SaMul-tang on Atopic Dermatitis Induced by DNCB in Mice

생료사물탕(生料四物湯)이 DNCB로 유발된 생쥐의 아토피 피부염에 미치는 영향

  • Kim, Ji-Soo (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Choi, Jung-Hwa (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Kim, Jong-Han (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Jeong, Min-Yeong (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Park, Soo-Yeon (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
  • 김지수 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 최정화 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 김종한 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 정민영 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 박수연 (동신대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2017.01.16
  • Accepted : 2017.02.16
  • Published : 2017.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Atopic Dermatitis (AD) is chronic skin disease characterized by allergic hypersensitivity reactions. Saeng-ryo-Samul-tang (SRSM) can treat skin disease by cooling down blood heat, cealering away congenital fever and detoxicating. This study was conducted to investigate the effects of SRSM on AD induced by 2,4-dinitrochlorobenzene (DNCB) in mice Methods : In this study, the effects of SRSM on changes in body weights, thicknesses of dorsum skin, thicknesses and weights of ear, changes of symptoms on the dorsum skin, histopathological degree of ear and dorsum skin, IL-10 and $TNF-{\alpha}$ in serum were observed. And the effects on the proliferation rates of splenocytes were also investigated in vivo and in vitro study. Results : In SRSM topical application (Topical) group, SRSM oral application (Oral) group and SRSM Combination (Combi) group thickness of dorsum skin decreased significantly. But in TPC, ORL and CBN group, weight of ear didn't show any changes, but thickness of ear decreased significantly. And TPC, ORL and CBN group showed meaningful effectiveness symptoms like desquamation and erythema on AD's clinical espect. In histopathological observation, spongiosis, edema and inflammatory cell infiltration of epidermal were remarkably diminished in TPC, ORL and CBN group. And SRSM diminished the proliferation rates of splenocytes in vivo and vitro study. Conclusions : The present study suggests that SRSM can significantly reduced symptoms of AD, therefore SRSM is effective to treatment of AD.

Keywords

References

  1. The Department of Dermatology of Seoul University College of Medicine. Dermatology For a medical student. 3rd ed. Seoul:Korea Medical Book Publishing Company. 2011:37-9.
  2. Kang YH. Atlas of skin disease. 2nd ed. Seoul:Hanmi Medical Publishing Co. 2008:83.
  3. Kang H, Kang HY, Park G, Park KC, Park MY, Seo YJ, et al. Text book of Dermatology. 6th ed. Seoul:Medbook. 2015:75-8,202,205.
  4. Kim KJ, Kim NK, Kim YB, Kim JH, Park MC, Park SY, et al. Text of Traditional Korean Dermatology & Surgery. Busan:Seonu. 2007:355-6.
  5. Ma SY. Hyeondaejunguipibuseongbyeonghag. Sanghae:Sanghae Junguiyagdaehagchulpansa. 2001:153-60.
  6. Cha KB, Kim YS, Seol IC. Literatual study on Atopic dermatitis. J Daejeon University Institute of Korean Medicine. 2005;14(2): 113-26.
  7. Yun YG. Donguibangjewa cheobanghaeseol. Seoul:Eui Seong Dang Publishing Co. 2007:250-1,281.
  8. Lee SH, Yun YG. Study for treatment of Atopic dermatitis in Oriental Medical Prescription. J Korean Med Ophtahlmol Otolaryngol Dermatol. 2006;19(3):103-17.
  9. Son MJ, Han JK, Kim YH. Suppressive Effect of SaengRyoSaMulTang On Activated RBL-2H3 Mast cells. J Korean Oriental Pediatrics. 2011; 25(1):28-39.
  10. Choe CG, Park EJ, Jeong GM. Study of the effect of Woohwangporhyonghwan(minus REALGAR and CINNABARIS) and Saengnyosamultang on the Anti-allergic in rats and mice. J Korean Oriental Pediatrics. 1993;7(1):1-16.
  11. Kim JJ, Yang SW, Son NW, Ahn KS. Anti-inflammatory action by Gamisangryosamutang and The effect of Ziyang-Go on atopic dermatitis-like lesion and pruritus in NC/Nga mice. Korean J Oriental Physiology & Pathology. 2003;17(2):428-35.
  12. Park SE, Heo Y. A study on the cause of the cause of atopic dermatitis. J Natural Sciences Research Institute. 2009;7(1):1-8.
  13. Eichenfield LF, Hanifin JM, Luger TA, Stevens SR, Pride HB. Consensus conference on pediatric atopic dermatitis. J Am Acad Dermatol. 2003;49(6):1088-95. https://doi.org/10.1016/S0190-9622(03)02539-8
  14. Kim GB, Joo DY. Junguipibubyeonghag. Beijing:China Medico Pharmaceutical Science & Technology Publishing House. 1999:193-8.
  15. Ma CY, Jo SH. Daejunguipibuseongbyeongjinlyodaejeon. Shanxi:Shanxi Science Technology Publishing House. 1999:475-93.
  16. Lee JC, Park SY, Choi JH, Kim JH. Effects of Portulacae Herba (PH) on Allergic Contact Dermatitis (ACD) Induced by DNCB in Mice. J Korean Med Ophthalmol Otolaryngol Dermatol. 2014;27(3):115-32. https://doi.org/10.6114/jkood.2014.27.3.115
  17. Kim SH. Effect of Chrysanthemi Borealis Flos on atopy dermatitis induced by DNCB in NC/Nga mouse. Kyunghee University Dissertation. 2010.
  18. Lee YS, Han JK, Kim YH. The Effects of Dictamni Radicis Cortex Extracts on DNCB Induced Atopic Dermatitis-like Skin Lesions of NC/Nga Mouse. J Pediatr Korean Med. 2014;28(4):1-29. https://doi.org/10.7778/JPKM.2014.28.4.001
  19. Lim KM. Effects of Adenophorae Radix(AR) on Atopic Dermatitis(AD) Induced by DNCB in Mice. Dongshin University Thesis. 2016.
  20. Park JM, Chae JW. Effects of Aurantii Immaturus Fructus (AI) on Atopic Dermatitis (AD) Induced by DNCB in Mice. J Pediatr Korean Med. 2015;29(1):27-43. https://doi.org/10.7778/jpkm.2015.29.1.027
  21. Jeong SI, Choi BM, Yun YG, Lee JW, Jang SI. A Noble Therapeutic Approach of Atopic dermatitis by Development of Th2 Chemokine Inhibitors from Natural Products : Inhibitory Effect of Sophora flavescens Extract in Atopic Dermatitis Model mice, NC/Nga. The Korean Journal of Oriental Medical Prescription. 2009;17(1):141-51.
  22. Sung YY, Yoon TS, Jang JY, Yang WK, Kim HK. Topical application of Rehmannia glutinosa extract inhibits mite allergeninduced atopic dermatitis in NC/Nga mice. J Korean Society of Medicinal Crop Science. 2010;349-50.
  23. Hwang CH, Yun CS, Song SH, Weon YH, Hwang CY. Effects of Galgeunhaegitanggamibang Administration along with Samhwangseze-gamibang on Atopic Dermatitis of NC/Nga Mice. J Korean Med Ophtahlmol Otolaryngol Dermatol. 2008;21(2):1-18.
  24. Yoon JE, Kim YH, Han JK. Effect of Gupoongjeseuptang (GPJST) on DNCB (dinitrochlorobenzene)-induced Atopic Derm. J Korean Oriental Pediatrics. 2008;22(3):105-37.
  25. Yang HJ. Effects of Gungguitang gamibang on atopic dermatitis induced by DNCB in mice. Wonkwang university Dissertation. 2014.
  26. Park DB, Han JK, Kim YH. Effect of Yanghyeuljeseuptang on immunological factors in spleen and draining lymph node(DLN) of atopic dermatitis induced NC/Nga mouse by dinitrochlorobenzene (DNCB). J Research Institute of Korean Medicine. 2007;16(2):251-65.
  27. Kim BA, Kim MS, Kang BM, Byeon SH, Park IH, Park JH, et al. Inhibitory Studies of Hwangryunhaedok-tang on Development of Atopic Dermatitis in NC/Nga Mice. Kor J Herbology. 2008;23(2):59-65.
  28. Hong CH, Seo ES, Weon YH, Kim YG, Hwang CY. Effects of Onchung-eum Administration along with Samhwangsezegamibang on Treatment Atopic Dermatitis Development in NC/Nga Mice. Korean J Oriental Physiology & Pathology. 2007;21(3):679-87.
  29. Khwag NG, Kang H, Myung EG, Park SM, Shim BS, Kim SH, et al. Effect of Yeongyupaedog-san on Cytokine Levels of Mouse Th1/Th2 Cells and Anti-allergic Activity in Ovalbumin-sensitized Allergic Inflammation Model. Korean J Oriental Physiology & Pathology. 2006;20(4):844-52.
  30. Seo HY, Han JK, Kim YH. The effect of Li Zhong Tang on the suppression of Th2 differentiation by IFN-$\gamma$ response in IgE hyperproduction and atopic dermatitis-like skin lesions induced NC/Nga mouce. J Korean Oriental Pediatrics. 2009;23(1):1-22.
  31. Park ES, Choi IH. The Effect of Sopungchengyoungtanggami-bang Extract to Recover Function of Stratum Corneum on Mice Model after Atopic Dermatitis Elicitation. J Korean Med Ophtahlmol Otolaryngol Dermatol. 2003;16(3):96-115.
  32. Kim SH, Choi JW, Kim JH, Park SY. Effects of Tang-gwi-eum-za-gagambang along with External Spray Therapy on the Spontaneously Occurring Atopic Dermatitis Development in NC/Nga Mouse. J Korean Med Ophtahlmol Otolaryngol Dermatol. 2005;18(1):27-49.
  33. Min YG, Choi JH, Kim JH, Park SY. Effects of Sopoongsangagambang administration along with external spray treatment on atopic dermatitis development in NC/Nga mice. J Korean Med Ophtahlmol Otolaryngol Dermatol. 2005;18(1):116-34.
  34. Hwang DY. Sinjeungbangyaghabpyeon. Seoul:Younglimsa. 2007:253.
  35. Kim IL, Kim HC, Kuk YB, Park SJ, Park YG, Park JH, et al. Bonchohag. Seoul:Jungumsa. 2011:162,177,217.
  36. Kim TY. Treatment of Atopic Dermatitis:an Update and Review of the Literature. Pediatric allergy and respiratory disease. 2009;19(3):209-19.
  37. Lee JH, Kim KH, Kim MN, Kim JW, Ro YS, Park YL, et al. Report from ADRG: The Treatment Guideline of Korean Atopic Dermatitis. Korean J Dermatol. 2006;44(8):907-13.
  38. Kim JK, Kim YH, Lee HH, Lim SS, Park KW. Effect of Scrophularia buergeriana Extract on the Degranulation of Mast Cells and Ear Swelling Induced by Dinitrofluorobenzene in Mice. Inflammation. 2011:12.
  39. Galli E, Cicconi R, Rossi P, Casati A, Brunetti E, Mancino G. Atopic dermatitis:molecular mechanisms, clinical aspects and new therapeutical approaches. Curr MolMed. 2003;3:127-38.
  40. Khayyamian S, Hutloff A, Buchner K, Grafe M, Henn V, Kroczek RA, et al. ICOS-ligand, expressed on human endothelial cells, costimulates Th1 and Th2 cytokine secretion by memory $CD4^+$ T cells. Proc Natl Acad Sci USA. 2002;99(9):6198-203. https://doi.org/10.1073/pnas.092576699
  41. Marzo AL, Vezys V, Williams K, Tough DF, Lefrancois L. Tissue-level regulation of Th1 and Th2 primary and memory CD4 T cells in response to Listeria infection. J Immunol. 2002;168(9):4504-10. https://doi.org/10.4049/jimmunol.168.9.4504
  42. Kim IS, Moon HJ. Essential Immunology. Seoul:Daihak Publishing Company. 2010:24-5.
  43. Jung IJ, Wang JH, Yun JW, Lee KW, Yeon SC, Lee HJ, et al. Radiographic Estimation of Experimentally Induced Splenomegaly in Dogs. Journal of Veterinary Clinics. 2006;23(3):263-6.
  44. Stasiuk LM, Abehsira-Amar O, Fournier C. Collagen-induced arthritis in DBA/1 mice;cytokine gene activation following immunization with type II collagen. Cell Immunol. 1996;173:269-75. https://doi.org/10.1006/cimm.1996.0277
  45. Kwon HH, Kim EJ, Kim IT, Park GH, Yang MG, Eom YB, et al. Clinical Immunology. Seoul:Korea Medical Book Publishing Company. 2010:166.