Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Measuring System of Visual Evoked Potential (VEP) in Mice using BioPAC Modules

BioPAC 모듈을 이용한 마우스 시각유발전위 측정 시스템 확립

  • Lee, Wang Woo (Department of Physiology, Chungbuk National University School of Medicine) ;
  • Ahn, Jung Ryul (Department of Physiology, Chungbuk National University School of Medicine) ;
  • Goo, Yong Sook (Department of Physiology, Chungbuk National University School of Medicine)
  • 이왕우 (충북대학교 의과대학 생리학교실) ;
  • 안정열 (충북대학교 의과대학 생리학교실) ;
  • 구용숙 (충북대학교 의과대학 생리학교실)
  • Received : 2017.01.02
  • Accepted : 2017.01.20
  • Published : 2017.02.28
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Abstract

For the development of feasible retinal prosthesis, one of the important elements is acquiring proper judging tool if electrical stimulus leads to patient's visual perception. If evoked potential to electrical stimulus is recorded in primary visual (V1) cortex, it means that the stimulus effectively evokes visual perception. Therefore, in this study, we established VEP recording system on V1 cortex using BioPAC modules as the judging tool. And the measuring system was evaluated by recording VEP of mice. After anesthesia, normal mice (C57BL/6J strain; n = 6) were secured to stereotaxic apparatus (Harvard Apparatus, USA). For the recording of VEP, the stainless steel needle electrode (impedance: $2-5k{\Omega}$) was positioned on the surface of the cortex through the burr hole at 2.5 mm lateral and 4.6 mm caudal to bregma. DA 100C and EEG 100C BioPAC modules were used for the trigger signal and VEP recording, respectively. When left eye was blocked by black cover and right eye was stimulated by flash light using HMsERG (RetVet Corp, USA), VEP response at left V1 cortex was detected, but there was no response at right V1 cortex. Amplitudes and latencies of P2, N3 peaks of VEP recording varied according to the depths of the electrodes on V1 cortex. From the surface upto $600{\mu}m$ depth, amplitudes of P2 and N3 increased, while deeper than $600{\mu}m$, those amplitudes decreased. The deeper the insertion depth of the electrode, the latency of N1 peaks tends to be delayed. However, there was no statistically significant difference among the latencies of P2 and N3 peaks (P > 0.05, ANOVA). Our VEP recording data such as the insertion depth and the latency and amplitudes of peaks might be used as guidelines for electrically-evoked potential (EEP) recording experiment in near future.

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