Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Microbial bioconversion of natural Philippine nut oils into a value-added hydroxy fatty acid, 7,10-dihydroxy-8(E)-octadecenoic acid

미생물 생변환을 통한 필리핀 너트유로부터 기능성 지방산 7,10-dihydroxy-8(E)-octadecenoic acid 생산

  • Dasangrandhi, Chakradhar (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Ellamar, Joel B. (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Young Soon (Department of Food and Nutrition, Korea University) ;
  • Kim, In Hwan (Department of Food and Nutrition, Korea University) ;
  • Kim, Hak-Ryul (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2016.09.08
  • Accepted : 2016.10.31
  • Published : 2017.02.28
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Abstract

Biocatalytic modification of natural resources can be used to generate novel compounds with specific properties, such as higher viscosity and reactivity. The production of hydroxy fatty acids (HFAs), originally found in low quantities in plants, is a good example of the biocatalytic modification of natural vegetable oils. HFAs show high potential for application in a wide range of industrial products, including resins, waxes, nylons, plastics, lubricants, cosmetics, and additives in coatings and paintings. In a recent study, Pseudomonas aeruginosa strain PR3 was used to produce 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) from oleic acid. This present study focused primarily on the utilization of three natural nut oils obtained from the Philippines -pili nut oil (PNO), palm oil (PO), and virgin coconut oil (VCO)- to produce DOD by P. aeruginosa strain PR3. Strain PR3 produced DOD from PNO and PO only, with PNO being the more efficient substrate. An optimization study to achieve the maximum DOD yield from PNO revealed the optimal incubation time and medium pH to be 48 h and 8.0, respectively. Among the carbon sources tested, fructose was the most efficiently used, with a maximum DOD production of 130 mg/50 mL culture. Urea was the optimal nitrogen source, with a maximum product yield of 165 mg/50 mL culture. The results from this study demonstrated that PNO could be used as an efficient substrate for DOD production by microbial bioconversion.

본 연구를 통하여 P. aeruginosa PR3를 이용하여 DOD를 생산하기 위해 저가의 기질로서 필리핀 너트유가 효과적으로 사용될 수 있음을 확인하였으며 배지에 첨가되는 여러 영양인자들의 영향을 조사하여 DOD 생산성을 크게 향상시킬 가능성이 있음도 확인하였다. 따라서 DOD 생산에 이용되는 올레산을 식물성오일로부터 별도의 생산과정을 거쳐 생산하지 않고 식물성오일자체를 직접 기질로 사용함으로서 PR3 균주를 이용하여 고부가가치의 DOD를 효율적으로 생산할 수 있다는 것을 확인하였다.

Keywords

References

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