한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제32권1호
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  • Pages.20-26
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  • 2017
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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국내 생산단계 농산물의 미생물학적 안전성 조사

Investigation of Microbiological Safety of on-farm Produce in Korea

  • 김원일 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 곽민규 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 조아라 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 류상돈 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김세리 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 류송희 (국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 김황용 (농촌진흥청 기술협력국 국외농업기술과) ;
  • 류재기 (국립농업과학원 농산물안전성부 유해생물팀)
  • Kim, Won-Il (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Gwak, Min-Gyu (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jo, A-Ra (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ryu, Sang Don (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Se-Ri (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ryu, Song Hee (Chemical Safety Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Hwang-Yong (Division for KOPIA (Korea Program on International Agriculture), Technology Cooperation Bureau, Rural Development Administration) ;
  • Ryu, Jae-Gee (Microbial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration)
  • 투고 : 2016.09.13
  • 심사 : 2016.12.06
  • 발행 : 2017.02.28
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초록

국제적으로 식중독 세균이 오염된 농산물의 신선 섭취에 따른 식중독 사고가 빈번하게 발생되고 있다. 본 연구는 국내 생산단계 수박, 참외, 고추, 당근, 상추, 배추, 양배추, 셀러리, 버섯, 보리, 옥수수, 복숭아, 포도를 대상으로 위생지표세균과 식중독 세균의 오염도를 분석하였다. 2013년부터 2015년까지 각 작물별로 3곳 이상의 주산지에서 총 1,820점의 시료를 수집하여 일반호기성세균, 대장균군, B. cereus, S. aureus의 정량적 분석과 E. coli, E. coli O157:H7, Salmonella spp., L. monocytogenes의 정성적 분석을 수행하였다. 일반호기성세균은 전체 시험대상 농산물에서 0.01~7.18 log CFU/g 범위의 밀도를 나타냈고, 대장균은 총 651점의(35.8%) 시료에서 나타났다. B. cereus는 0.01~2.48 log CFU/g 범위로 총 169점의(9.3) 시료에서 나타났고, S. aureus는 총 14점의(0.7%) 시료에서 0.01~1.69 log CFU/g 범위로 나타났다. 정성적 분석에서 E. coli는 총 101점의(5.5%) 시료에서 양성인 것으로 나타났고, E. coli O157:H7, Salmonella spp., L. monocytogenes는 모두 음성인 것으로 나타났다. 본 연구 결과를 통해 국내 생산단계 농산물의 전반적인 미생물학적 오염도를 파악할 수 있고 농산물 중 미생물 기준 설정 등에 대한 과학적 근거로써 미생물위험평가(Microbiological Risk Assessment)의 기초자료로써 활용이 가능할 것으로 기대한다.

Foodborne disease outbreaks associated with produces have been increasing in occurrence worldwide. This study investigated microbial contamination levels on thirteen kinds of agricultural products from farms stage to evaluate potential hazards associated with foodborne illness. A total of 1,820 samples were collected in major cultivating area from 2013 through 2015, and analyzed to enumerate aerobic bacterial counts, coliforms/E. coli, Bacillus cereus and Staphylococcus aureus. In addition, the prevalence study for four kinds of microorganisms (Escherichia coli, E. coli O157:H7, Salmonella spp. and Listeria monocytogenes) was performed on each sample. Aerobic bacterial counts ranged from 0.01 to 7.18 log CFU/g, with the highest bacterial cell counts recorded for watermelon. Coliforms were detected in 651 samples (35.8%) with a minimum of 0.01 log CFU/g and a maximum of more than 5 log CFU/g. B. cereus was detected in 169 samples (9.3%) ranging from < 0.01 to 2.48 log CFU/g among total samples analyzed. S. aureus was detected in 14 samples (0.7%) with a minimum of 0.01 log CFU/g and a maximum of 1.69 log CFU/g. E. coli was detected in 101 samples (5.5%) among 1,820 samples. E. coli O157:H7, Salmonella spp. and L. monocytogenes were not detected in any of the samples. The microbial contamination levels of several agricultural products determined in this study may be used as the fundamental data for microbiological risk assessment (MRA).

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피인용 문헌

  1. Evaluation on Microbial Contamination in Red Pepper and Red Pepper Cultivated Soil in Korea vol.33, pp.5, 2018, https://doi.org/10.13103/JFHS.2018.33.5.347
  2. Changes of Microbial Populations on Major Leafy Vegetables Cultivated by Different Methods from Production to Washing Stages vol.33, pp.1, 2018, https://doi.org/10.13103/JFHS.2018.33.1.38