DOI QR코드

DOI QR Code

Microleakage Assessment of a Pozzolan Cement-based Mineral Trioxide Aggregate Root Canal Sealer

포졸란 시멘트를 기반으로 하는 근관전색제의 치근단부 미세누출 평가

  • Kim, Mijun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Howon (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Juhyun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Seo, Hyunwoo (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
  • 김미준 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 박호원 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 이주현 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 서현우 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소)
  • Received : 2016.03.09
  • Accepted : 2016.05.25
  • Published : 2017.02.28

Abstract

This study aimed to assess microleakage of Endoseal MTA when it is used as a root canal sealer and a root canal filling material compared with conventional endodontic treatment materials Forty-two mature human permanent teeth with a single root canal were divided randomly into three experimental groups (n = 10) and two control groups (n = 6). Group A was obturated with AH $plus^{(R)}$ and gutta-percha (GP). Group E1 was obturated with Endoseal MTA and GP. Group E2 was obturated with Endoseal MTA only. The positive control group was obturated with GP only and the negative control group was obturated in the same way as the experimental groups. The samples were kept in saline solution for 24 hours and were immersed in 0.2% rhodamine B dye solution for 24 hours. Then the samples were split longitudinally and the micoleakage was assessed under a stereomicroscope. Complete microleakage was detected in all positive control group samples, whereas no microleakage was detected in the negative control group. There was no statistically significant difference between the experimental groups in the Kruskal-Wallis test. These results suggest that Endoseal MTA has potential use as a root canal sealer and a root canal filling material.

본 연구는 포졸란 시멘트를 기반으로 하는 근관전색제인 Endoseal MTA (Maruchi, Wonju, Korea)를 근관전색제와 근관충전제로 사용하였을 때 나타나는 치근단부 미세누출을 기존의 근관전색제인 AH $Plus^{(R)}$ (Dentsply DeTrey, Konstanz, Germany)와 비교하여 평가하고자 하였다. 만곡되지 않은 영구 전치를 3개의 실험군(A군, E1군, E2군)으로 나누고 A군은 AH $Plus^{(R)}$와 gutta-percha (GP), E1군 은 Endoseal MTA와 GP, E2군은 Endoseal MTA 단독으로 충전하였다. 충전이 완료된 치아는 완전한 경화를 위해 24시간동안 멸균 증류수에 보관하였고 염색액 누출을 위해 치근단 부위를 0.2% 로다민 B 염색 용액에 24시간 동안 노출시켰다. 입체 현미경을 이용하여 미세누출 정도를 평가하였으며 3개의 실험군 사이에 유의한 차이가 관찰되지 않았다. 결론적으로 Endoseal MTA는 GP의 사용에 관계 없이 AH $Plus^{(R)}$와 유사한 밀폐력을 보였으며 이러한 결과는 Endoseal MTA의 근관전색제 및 근관 충전제로써 유용한 사용에 대한 가능성을 제시한다.

Keywords

References

  1. Hoen MM, Pink FE : Contemporary endodontic retreatments: an analysis based on clinical treatment findings. J Endod, 28:834-836, 2002. https://doi.org/10.1097/00004770-200212000-00010
  2. Siqueira JF, Jr., Rocas IN : Clinical implications and microbiology of bacterial persistence after treatment procedures. J Endod, 34:1291-1301 e1293, 2008. https://doi.org/10.1016/j.joen.2008.07.028
  3. Ng YL, Mann V, Rahbaran S, et al. : Outcome of primary root canal treatment: systematic review of the literature -- Part 2. Influence of clinical factors. Int Endod J, 41:6-31, 2008.
  4. Torabinejad M, Chivian N : Clinical applications of mineral trioxide aggregate. J Endod, 25:197-205, 1999. https://doi.org/10.1016/S0099-2399(99)80142-3
  5. Hachmeister DR, Schindler WG, Walker WA, Thomas DD : The sealing ability and retention characteristics of mineral trioxide aggregate in a model of apexification. J Endod, 28:386-390, 2002. https://doi.org/10.1097/00004770-200205000-00010
  6. Vizgirda PJ, Liewehr FR, Patton WR, et al. : A comparison of laterally condensed gutta-percha, thermoplasticized gutta-percha, and mineral trioxide aggregate as root canal filling materials. J Endod, 30:103-106, 2004.
  7. Aminoshariae A, Hartwell GR, Moon PC : Placement of mineral trioxide aggregate using two different techniques. J Endod, 29:679-682, 2003. https://doi.org/10.1097/00004770-200310000-00017
  8. Lawley GR, Schindler WG, Walker WA, Kolodrubetz D : Evaluation of ultrasonically placed MTA and fracture resistance with intracanal composite resin in a model of apexification. J Endod, 30:167-172, 2004. https://doi.org/10.1097/00004770-200403000-00010
  9. Yeung P, Liewehr FR, Moon PC : A quantitative comparison of the fill density of MTA produced by two placement techniques. J Endod, 32:456-459, 2006. https://doi.org/10.1016/j.joen.2005.08.008
  10. Malhotra VM, Mehta PK : Pozzolanic and cementitious materials. Amsterdam, The Netherlands: Gordon and Breach; 1996.
  11. Choi Y, Park SJ, Lee SH, et al. : Biological effects and washout resistance of a newly developed fastsetting pozzolan cement. J Endod, 39:467-472, 2013. https://doi.org/10.1016/j.joen.2012.11.023
  12. Jang GY, Park SJ, Heo SM, et al. : Washout resistance of fast-setting pozzolan cement under various root canal irrigants. Restor Dent Endod, 38:248-252, 2013. https://doi.org/10.5395/rde.2013.38.4.248
  13. Kim M, Yang W, Kim H, Ko H : Comparison of the biological properties of ProRoot MTA, OrthoMTA, and Endocem MTA cements. J Endod, 40:1649-1653, 2014. https://doi.org/10.1016/j.joen.2014.04.013
  14. Song M, Yoon TS, Kim SY, Kim E : Cytotoxicity of newly developed pozzolan cement and other root-end filling materials on human periodontal ligament cell. Restor Dent Endod, 39:39-44, 2014. https://doi.org/10.5395/rde.2014.39.1.39
  15. Han L, Kodama S, Okiji T : Evaluation of calciumreleasing and apatite-forming abilities of fast-setting calcium silicate-based endodontic materials. Int Endod J, 48:124-130, 2015. https://doi.org/10.1111/iej.12290
  16. Park SJ, Heo SM, Hong SO, et al. : Odontogenic effect of a fast-setting pozzolan-based pulp capping material. J Endod, 40:1124-1131, 2014. https://doi.org/10.1016/j.joen.2014.01.004
  17. Jang JH, Kang M, Ahn S, et al. : Tooth discoloration after the use of new pozzolan cement (Endocem) and mineral trioxide aggregate and the effects of internal bleaching. J Endod, 39:1598-1602, 2013. https://doi.org/10.1016/j.joen.2013.08.035
  18. Oppenheimer S, Rosenberg PA : Effect of temperature change on the sealing properties of Cavit and Cavit G. Oral Surg Oral Med Oral Pathol, 48:250-253, 1979. https://doi.org/10.1016/0030-4220(79)90012-4
  19. Wu MK, Kontakiotis EG, Wesselink PR : Decoloration of 1% methylene blue solution in contact with dental filling materials. J Dent, 26:585-589, 1998. https://doi.org/10.1016/S0300-5712(97)00040-7
  20. Vogt BF, Xavier CB, Demarco FF, Padilha MS : Dentin penetrability evaluation of three different dyes in root-end cavities filled with mineral trioxide aggregate (MTA). Braz Oral Res, 20:132-136, 2006. https://doi.org/10.1590/S1806-83242006000200008
  21. Tanomaru Filho M, Figueiredo FA, Tanomaru JM : Effect of different dye solutions on the evaluation of the sealing ability of Mineral Trioxide Aggregate. Braz Oral Res, 19:119-122, 2005. https://doi.org/10.1590/S1806-83242005000200008
  22. Gomes-Filho JE, Moreira JV, Watanabe S, et al. : Sealability of MTA and calcium hydroxidecontaining sealers. J Appl Oral Sci, 20:347-351, 2012. https://doi.org/10.1590/S1678-77572012000300009
  23. Samiei M, Aghazade M, Farhadi F, et al. : Sealing efficacy of single-cone obturation technique with MTA and CEM cement: An in vitro bacterial leakage study. J Dent Res Dent Clin Dent Prospects, 8:77-83, 2014.
  24. Antonopoulos KG, Attin T, Hellwig E : Evaluation of the apical seal of root canal fillings with different methods. J Endod, 24:655-658, 1998. https://doi.org/10.1016/S0099-2399(98)80149-0
  25. McMichen FR, Pearson G, Rahbaran S, Gulabivala K : A comparative study of selected physical properties of five root-canal sealers. Int Endod J, 36:629-635, 2003. https://doi.org/10.1046/j.1365-2591.2003.00701.x
  26. Gogos C, Economides N, Stavrianos C, et al. : Adhesion of a new methacrylate resin-based sealer to human dentin. J Endod, 30:238-240, 2004. https://doi.org/10.1097/00004770-200404000-00014
  27. Erdemir A, Adanir N, Belli S : In vitro evaluation of the dissolving effect of solvents on root canal sealers. J Oral Sci, 45:123-126, 2003. https://doi.org/10.2334/josnusd.45.123
  28. Hwang JH, Chung J, Na HS, et al. : Comparison of bacterial leakage resistance of various root canal filling materials and methods: Confocal laser-scanning microscope study. Scanning, 37;422-428, 2015. https://doi.org/10.1002/sca.21231
  29. Donnelly A, Sword J, Nishitani Y, et al. : Water sorption and solubility of methacrylate resin-based root canal sealers. J Endod, 33:990-994, 2007. https://doi.org/10.1016/j.joen.2006.03.021
  30. Lim ES, Park YB, Kwon YS, et al. : Physical properties and biocompatibility of an injectable calciumsilicate-based root canal sealer: in vitro and in vivo study. BMC Oral Health, 15:129, 2015. https://doi.org/10.1186/s12903-015-0112-9
  31. Fridland M, Rosado R : Mineral trioxide aggregate (MTA) solubility and porosity with different waterto-powder ratios. J Endod, 29:814-817, 2003. https://doi.org/10.1097/00004770-200312000-00007
  32. Sonmez IS, Oba AA, Sonmez D, Almaz ME : In vitro evaluation of apical microleakage of a new MTAbased sealer. Eur Arch Paediatr Dent, 13:252-255, 2012. https://doi.org/10.1007/BF03262880
  33. Ehsani M, Dehghani A, Abesi F, et al. : Evaluation of Apical Micro-leakage of Different Endodontic Sealers in the Presence and Absence of Moisture. J Dent Res Dent Clin Dent Prospects, 8:125-129, 2014.
  34. Pawar SS, Pujar MA, Makandar SD : Evaluation of the apical sealing ability of bioceramic sealer, AH plus & epiphany: An in vitro study. J Conserv Dent, 17:579-582, 2014. https://doi.org/10.4103/0972-0707.144609