Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Inhibition of NO Production by Ethanol Extract of Prunus mume Fruits in LPS-Stimulated RAW 264.7 Macrophages through Regulation of the Nrf2/HO-1 Signaling Pathway

LPS가 처리된 RAW 264.7 대식세포에서 Nrf2/HO-1 경로 조절을 통한 매실 추출물의 NO 생성 억제 효과

  • Kang, Hye-Joo (Anti-Aging Research Center, Dong-Eui University) ;
  • Choi, Eun Ok (Anti-Aging Research Center, Dong-Eui University) ;
  • Jeong, Jin-Woo (Anti-Aging Research Center, Dong-Eui University) ;
  • Park, Shin-Hyung (Department of Pathology, College of Korean Medicine, Dong-Eui University) ;
  • Park, Cheol (Department of Molecular Biology, College of Natural Sciences, Dong-Eui University) ;
  • Hong, Su Hyun (Department of Biochemistry, College of Korean Medicine, Dong-Eui University) ;
  • Shin, Soon Shik (Department of Formula Sciences, College of Korean Medicine, Dong-Eui University) ;
  • Cheong, Jae-Hun (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
  • Choi, Yung Hyun (Anti-Aging Research Center, Dong-Eui University)
  • 강혜주 (동의대학교 항노화연구소) ;
  • 최은옥 (동의대학교 항노화연구소) ;
  • 정진우 (동의대학교 항노화연구소) ;
  • 박신형 (동의대학교 한의과대학 병리학교실) ;
  • 박철 (동의대학교 자연생활과학대학 분자생물학과) ;
  • 홍수현 (동의대학교 한의과대학 생화학교실) ;
  • 신순식 (동의대학교 한의과대학 방제학교실) ;
  • 정재훈 (부산대학교 자연과학대학 분자생물학과) ;
  • 최영현 (동의대학교 항노화연구소)
  • Received : 2017.01.31
  • Accepted : 2017.02.10
  • Published : 2017.02.28
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Abstract

Objectives : The fruit of Prunus mume Siebold & Zucc. has been used as an alternative medicine and functional food in Korea and Japan for preventive and therapeutic purposes. However, its molecular actions and mechanism on anti-inflammatory activity have not been clearly investigated. The aim of this study was to clarify the anti-inflammatory activity of the ethanol extract of P. mume fruit (EEPM) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, and sought to understand the associated molecular mechanisms. Methods : Cytotoxicity was assessed by an MTT assay. The amount of nitric oxide (NO) production was determined by nitrite assay. The mRNA expression of inducible nitric oxide synthase (iNOS) was analyzed by RT-PCR. In addition, expression levels of iNOS, nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) protein were detected by Western blotting. Results : Our data indicated that EEPM inhibited NO production in LPS-stimulated RAW264.7 cells in a concentration-dependent manner. At the mRNA and protein levels, EEPM suppressed LPS-induced iNOS expression. On the other hand, EEPM markedly enhanced HO-1 expression, which was associated with an induction and nuclear translocation of Nrf2. Moreover, the inhibitory effect of EEPM against LPS‑induced NO production was significantly enhanced by hemin, a HO-1 inducer; however, EEPM's effect on the production of NO was abolished by zinc protoporphyrin IX, a HO-1 inhibitor. Conclusion : The results suggest that EEPM can act as a suppressor agent on NO production through an activation of Nrf2/HO-1 signaling pathway, and may be a promising candidate for the treatment of inflammatory diseases.

Keywords

References

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