Journal of Microbiology and Biotechnology

  • 제27권2호
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  • Pages.405-411
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  • 2017
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Hed1 Promotes Meiotic Crossover Formation in Saccharomyces cerevisiae

  • Kong, Yoon-Ju (Department of Life Sciences, Chung-Ang University) ;
  • Joo, Jeong-Hwan (Department of Life Sciences, Chung-Ang University) ;
  • Kim, Keun Pil (Department of Life Sciences, Chung-Ang University) ;
  • Hong, Soogil (Department of Life Sciences, Chung-Ang University)
  • 투고 : 2016.10.28
  • 심사 : 2016.12.02
  • 발행 : 2017.02.28
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초록

Homologous recombination occurs between homologous chromosomes and is significantly involved in programmed double-strand break (DSB) repair. Activation of two recombinases, Rad51 and Dmc1, is essential for an interhomolog bias during meiosis. Rad51 participates in both mitotic and meiotic recombination, and its strand exchange activity is regulated by an inhibitory factor during meiosis. Thus, activities of Rad51 and Dmc1 are coordinated to promote homolog bias. It has been reported that Hed1, a meiosis-specific protein in budding yeast, regulates Rad51-dependent recombination activity. Here, we investigated the role of Hed1 in meiotic recombination by ectopic expression of the protein after pre-meiotic replication in Saccharomyces cerevisiae. DNA physical analysis revealed that the overexpression of Hed1 delays the DSB-to-joint molecule (JM) transition and promotes interhomolog JM formation. The study indicates a possible role of Hed1 in controlling the strand exchange activity of Rad51 and, eventually, meiotic crossover formation.

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피인용 문헌

  1. Roles of Budding Yeast Hrr25 in Recombination and Sporulation vol.27, pp.6, 2017, https://doi.org/10.4014/jmb.1701.01016