Journal of dental hygiene science (치위생과학회지)

The Korean Society of Dental Hygiene Science (한국치위생과학회)
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Effects of Red or Black Ginseng Extract in a Rat Model of Inflammatory Temporomandibular Joint Pain

흰 쥐의 턱관절 염증성 통증모델에서 홍삼 및 흑삼추출물의 효과

  • Lee, Hyeon-Jeong (Department of Biomedical Health Science, Graduate School, Dong-Eui University) ;
  • Kim, Yun-Kyung (Department of Biomedical Health Science, Graduate School, Dong-Eui University) ;
  • Choi, Ja-Hyeong (Department of Biomedical Health Science, Graduate School, Dong-Eui University) ;
  • Lee, Jung-Hwa (Department of Biomedical Health Science, Graduate School, Dong-Eui University) ;
  • Kim, Hye-Jin (Department of Biomedical Health Science, Graduate School, Dong-Eui University) ;
  • Seong, Mi-Gyung (Department of Dental Hygiene, Masan University) ;
  • Lee, Min-Kyung (Department of Biomedical Health Science, Graduate School, Dong-Eui University)
  • 이현정 (동의대학교 일반대학원 보건의과학과) ;
  • 김윤경 (동의대학교 일반대학원 보건의과학과) ;
  • 최자형 (동의대학교 일반대학원 보건의과학과) ;
  • 이정화 (동의대학교 일반대학원 보건의과학과) ;
  • 김혜진 (동의대학교 일반대학원 보건의과학과) ;
  • 성미경 (마산대학교 치위생과) ;
  • 이민경 (동의대학교 일반대학원 보건의과학과)
  • Received : 2016.12.08
  • Accepted : 2017.01.11
  • Published : 2017.02.28
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Abstract

Temporomandibular joint (TMJ) pain is characterized by persistent jaw pain associated with dysfunction and tenderness of the temporomandibular muscles and joints. The aim of this study was to investigate whether treatment with red or black ginseng extract helps in the modulation of inflammatory TMJ pain. Male Sprague-Dawley rats weighing 220~260 g were used. The experimental group was subdivided into 4 groups based on the treatment method (n=6, each group): formalin (5%, $30{\mu}l$), formalin after distilled water (vehicle), formalin after red or black ginseng extract (per oral, single or repeated, respectively). To induce TMJ pain, $30{\mu}l$ of formalin was injected into the articular cavity under ether inhalation anesthesia. The number of noxious behavioral responses of scratching the facial region proximal to the injection site was recorded for 9 successive 5-min intervals following formalin injection. Repeated treatment with red or black ginseng extract reduced the nociceptive responses in the second phase (11~45 min). Nuclear factor erythroid 2-related factor 2 (Nrf2) is an oxidative stress-mediated transcription factor. Both ginsengs significantly down-regulated the increased Nrf2 level compared to the vehicle group. In the test for liver and kidney functions, repeated treatment with red or black ginseng was not different compared to the vehicle group. These results indicate that red and black ginseng extract might be promising analgesic agents in the treatment of inflammatory TMJ pain.

실험동물의 TMJ 내에 포르말린으로 유도한 염증성 통증 모델에서 홍삼 및 흑삼 추출물이 통증 발생과 조절에 미치는 영향을 평가하고자 하였다. 홍삼 혹은 흑삼 추출물을 TMJ 내에 투여한 후 통증 행위반응의 변화를 관찰하였고, 또한 실험동물의 연수를 적출하여 Nrf2 경로의 활성 변화를 단백정량분석법을 활용해서 분석한 결과 먼저, 포르말린을 TMJ에 주입한 결과 안면부 통증행위반응이 유의하게 증가되었다. 이것을 토대로 홍삼 및 흑삼 추출물의 경구 투여한 결과 포르말린에 의해 유도된 실험동물에서 통증행위 반응이 효과적으로 감소되었고, 단회투여보다 반복투여가 포르말린 2차 통증행위반응 경감에 더 효과적으로 나타났다. 또한 홍삼 및 흑삼 추출물의 반복 투여 시 포르말린에 주입에 의해 증가된 연수에서의 Nrf2 단백 발현량을 감소시켰고, 간과 신장의 독성검사에서도 영향을 미치지 않는 것으로 나타났다. 따라서, 본 연구에서 홍삼 및 흑삼 추출물은 간과 신장을 부작용을 나타내지 않으면서 포르말린으로 유도된 안면부 통증과 Nrf2의 발현의 조절에 효과적인 것으로 나타났다.

Keywords

References

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