Nutritional Quality and Physicochemical Characteristics of Defatted Bovine Liver Treated by Supercritical Carbon Dioxide and Organic Solvent

  • Kang, Sung-Won (Division of Applied Life Science (BK21 Plus), Gyeongsang National University) ;
  • Kim, Hye-Min (Division of Applied Life Science (BK21 Plus), Gyeongsang National University) ;
  • Rahman, M. Shafiur (Division of Applied Life Science (BK21 Plus), Gyeongsang National University) ;
  • Kim, Ah-Na (Division of Applied Life Science (BK21 Plus), Gyeongsang National University) ;
  • Yang, Han-Sul (Division of Applied Life Science (BK21 Plus), Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Food Science and Technology (Institute of Agriculture and Life Sciences), Gyeongsang National University)
  • Received : 2016.11.15
  • Accepted : 2016.12.15
  • Published : 2017.02.28


Defatted bovine liver (DBL) is a potential source of protein and minerals. Supercritical carbon dioxide ($SC-CO_2$) and a traditional organic solvent method were used to remove lipid from bovine liver, and the quality characteristics of a control bovine liver (CBL), bovine liver defatted by $SC-CO_2$ ($DBLSC-CO_2$) at different pressures, and bovine liver defatted by organic solvent (DBL-OS) were compared. The $DBLSC-CO_2$ samples had significantly higher (p<0.05) protein, amino acid, carbohydrate, and fiber contents than CBL and DBL-OS. There was a higher yield of lipid from CBL when using $SC-CO_2$ than the organic solvent method. SDS-PAGE analysis demonstrated that the CBL and $DBLSC-CO_2$ had protein bands of a similar intensity and area, whereas DBL-OS appeared extremely poor bands or no bands due to the degradation of proteins, particularly in the 50 to 75 kDa and 20 to 25 kDa molecular weight ranges. In addition, $DBLSC-CO_2$ was shown to have superior functional properties in terms of total soluble content, water and oil absorption, and foaming and emulsification properties. Therefore, $SC-CO_2$ treatment offers a nutritionally and environmentally friendly approach for the removal of lipid from high protein food sources. In addition, $SC-CO_2$ may be a better substitute of traditional organic solvent extraction for producing more stable and high quality foods with high-protein, fat-free, and low calorie contents.



Supported by : Korea Institute of Planning and Evaluation for Technology in Food and Agriculture, Forestry and Fisheries


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