The Korean Journal of Community Living Science (한국지역사회생활과학회지)

The Korean Society of Community Living Science (한국지역사회생활과학회)
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Genotoxicological Safety Evaluation of Crude Antifungal Compounds Produced by Bacillus subtilis SN7

Bacillus subtilis SN7이 생성한 조항균 물질의 유전독성학적 안정성평가

  • 장해춘 (조선대학교 식품영양학과) ;
  • 고상범 (한국화학융합시험연구원 헬스케어연구소) ;
  • 이재준 (조선대학교 식품영양학과)
  • Received : 2017.01.17
  • Accepted : 2017.02.24
  • Published : 2017.02.28
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Abstract

This study was carried out to perform genotoxicological safety evaluation of crude antifungal compounds produced by Bacillus subtilis SN7 (B. subtilis SN7) isolated from meju. Bacterial reverse mutation assay with Salmonella typhimurium TA98, TA100, TA1535, and TA1537 or Escherichia coli WP2uvrA in the presence and absence of the S9 metabolic activation system was carried out, and the crude antifungal compounds produced by B. subtilis SN7 showed no significant increase in the number of revertant colonies. In the chromosomal aberration tests using Chinese hamster lung (CHL) cells, sample treatment groups showed no increase in the frequency of chromosome aberrations compared to the negative control group. Furthermore, in the micronucleus formation test, the crude antifungal compounds showed no significance increase in the frequency of polychromatic erythrocytes with micronuclei. These results suggest that the crude antifungal compounds produced by B. subtilis SN7 isolated from meju showed no harmful genotoxic effects.

Keywords

References

  1. Ames BN, McCann J, Yamasaki E(1975) Methods for detecting carcinogens and mutagens with Salmonella/mammalian - microsome mutagenicity test. Mutation Res 31(6), 347-364 https://doi.org/10.1016/0165-1161(75)90046-1
  2. Bennett JW, Klich M(2003) Mycotoxins. Clin Microbiol Rev 16(3), 497-516 https://doi.org/10.1128/CMR.16.3.497-516.2003
  3. Chang HC, Koh SB, Lee JJ(2016) Single oral dose toxicity test and four weeks repeated oral dose determination test of crude antifungal compounds produced by Bacillus subtilis SN7 in rats. Korean J Community Living Sci 27(3), 437-449 https://doi.org/10.7856/kjcls.2016.27.3.437
  4. Dean BJ, Danford N(1984) Assays for the detection of chemically-induced chromosome damage in cultured mammalian cells. In: Mutagenicity testing - a practical approach, Venitt S & Parry JM(Editors), IRL Press Limited, Eynsham, England pp187-232
  5. Drider D, Fimland G, Hechard Y., McMullen LH, Prevost H(2006) The continuing story of class IIa bacteriocins. Microbiol Mol Biol Rev 70(2), 564-582 https://doi.org/10.1128/MMBR.00016-05
  6. Hayashi M(1991) The micronucleus test. Science Press Inc., Tokyo, Japan. p 65-69
  7. Hayashi M, Yoshimura I, Sofuni T, Ishidate M Jr(1989) A procedure for data analysis of the rodent micronucleus test involving a historical control. Environ Mol Mutagen 13(4), 347-356 https://doi.org/10.1002/em.2850130412
  8. Hong SG, Chung SG, Hyun SH(2008) The micronucleus test of the diglyceride preparation with conjugated linoleic acid by using mice. J Korean Soc Food Sci Nutr 37(7), 853-857 https://doi.org/10.3746/jkfn.2008.37.7.853
  9. Huang YH, Jung DW, Kang IJ(2014) Genotoxicity safety evaluation of imported oranges irradiated with ionizing energy. J Korean Soc Food Sci Nutr 43(6), 909-915 https://doi.org/10.3746/jkfn.2014.43.6.909
  10. Ishidate M Jr, Sofuni T, Yoshikawa K(1981) Chromosomal aberration tests in vitro as a primary screening tool for environmental mutagens and/or carcinogens. Gann Monogr Cancer Res 27(1), 95-107
  11. Jack RW, Tagg JR, Ray B(1995) Bacteriocin of grampositive bacteria. Microbiol Rev 59(2), 171-200
  12. Kim KH, Jeong CH, Joo SJ, Park JH, Moon JY, Cho EJ, Lee HT, Kwon HJ, Kim BW, Eom SH, Lee EW(2015) Single dose oral toxicity of Baciluus subtilis JNS in ICR mice. J Korean Soc Food Sci Nutr 44(1), 24-28 https://doi.org/10.3746/jkfn.2015.44.1.024
  13. Lee SG(2014) Isolation and characterization of Baciluus subtilis harboring anti-Baciluus cereus activity from meju and purification of its antimicrobial compound. MS Dissertation, Chosun university
  14. Lee GG, Lee HD, Lee CH(2003) Changes in sensory characteristics during salt aging of doenjang (fermented soybean paste) made by different starters. Food Eng Prog 7(1), 13-19
  15. Lim JH, Park BK, Kim MS, Rhee MH, Park SC, Yun HI(2004) Acute oral toxicity and pathogenicity of a potential probiotic Bacillus sp. A9184 isolated from soybean paste. J Toxicol Pub Health 20(4), 359-363
  16. Melin P, Sundh I, Hakansson S, Schnurer J(2007) Biological preservation of plant derived animal feed with antifungal microorganisms: safety and formulation aspects. Biotechnol Lett 29(8), 1147-1154 https://doi.org/10.1007/s10529-007-9375-9
  17. Maron, DM, Ames BN(1983) Revised methods for the Salmonella mutagenicity test. Mutat Res 113(3-4): 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  18. Ministry of Food and Drug Safety(2014) Guideline for toxicity tests of drugs and related materials notification. Chungbuk, MFDS
  19. Moss MO (2008) Fungi, quality and safety issues in fresh fruits and vegetables. J Appl Microbiol 104(5), 1239-1243 https://doi.org/10.1111/j.1365-2672.2007.03705.x
  20. Schallmey M, Singh A, Ward OP(2004) Development in the use of Bacillus species for industrial production. Can J Microbiol 50(1), 1-17 https://doi.org/10.1139/w03-076
  21. Sharp RJ, Scawen MD, Atkinson T(1989) Fermentation and downstream processing of Baciluus. Biotechnology Handbooks 2. Springer US, New York, USA pp255-292
  22. Song HP, Shin EH, Yun H, Cho C, Kim D(2009) Establishing the genotoxicological safety of gammairradiated egg white and yolk. Korean J Food Preserv 16(5), 782-788
  23. Tagg JR, Dajani AS, Wannamaker LW(1976) Bacteriocins of gram-positive bacteria. Bacteriol Rev 40(3), 722-756