Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Expression pattern of low-temperature-related genes by the treatment of AFP in the cryopreservation of potato shoot tips

감자 정아 동결보존과정에서 AFP 처리 시 저온관련 유전자의 발현양상

  • Seo, Ji-Hyang (Department of Horticulture, Kyungpook National University) ;
  • Jeon, Su Min (Department of Horticulture, Kyungpook National University) ;
  • Naing, Aung Htay (Department of Horticulture, Kyungpook National University) ;
  • Han, Jeung Sul (Department of Horticulture, Kyungpook National University) ;
  • Park, In Hwan (Department of Landscape Architecture, Kyungpook National University) ;
  • Kim, Chang-Kil (Department of Horticulture, Kyungpook National University)
  • Received : 2017.12.11
  • Accepted : 2017.12.18
  • Published : 2017.12.31
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Abstract

The expression profiles of low-temperature-related genes were examined in in vitro potatoes exposed to a cold condition for 1-3 days. The expression levels of PPI1 and CI21B genes were lineraly elevated from day 1 to day 3, while the opposite trend was observed for CBF4 and CI21A. In addition, the expression of the genes CI21A and CI21B varied, along with specific tissues (leaf, stem, and tuber) and the treatment periods. Therefore, potato shoot tips were cryopreserved with LS and PVS2 containing different oncentrations of AFP. It can thus be inferred that the presence of AFP in LS and PVS2 was likely to elevate expression pattern of the genes. Furthermore, the concentration of AFP (1,500 ng/ml for LS and 500 ng/mg for PVS2) was the best for the cryopreservation of potatoes.

기내배양한 감자에 1일, 2일, 3일간 저온처리 후 저온관련 유전자의 발현양상을 조사하였다. 저온 처리 기간이 늘어날수록 PPI1, CI21B유전자의 발현양은 증가하였고, CBF4, CI21A 유전자의 발현양은 감소하였다. 또한 CI21A와 CI21B유전자의 잎, 줄기, 괴경에서 명 처리와 암 처리 기간별로 저온 처리기간에 따른 유전자의 발현의 차이를 보았다. 이에 근거하여 감자 정아의 동결과정 중에서 LS단계와 PVS2 단계에서 AFP를 농도별로 처리하였다. 그 결과, 저온처리 기간이 늘었을 때의 유전자 발현양상과 AFP 특정 농도에서의 발현 양상이 동일하였다. LS단계에서는 1,500 ng/ml AFP 처리와, PVS2단계에서 500 ng/ml AFP 처리가 감자 정아의 초저온동결보존에 효과적이라는 것을 유전자의 발현 양상으로 확인하였다.

Keywords

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