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신갈나무 추출물의 안전성 및 항산화성

Antioxidant and Safety Test of Natural Extract of Quercus mongolica

  • 정지영 (경상대학교 농업생명과학대학 환경재료과학과, 농업생명과학연구원) ;
  • 양재경 (경상대학교 농업생명과학대학 환경재료과학과, 농업생명과학연구원) ;
  • 이원희 (경북대학교 농업생명과학대학 임산공학과)
  • Jung, Ji-Young (Department of Environmental Materials Science, College of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Yang, Jae-Kyung (Department of Environmental Materials Science, College of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Lee, Won-Hee (Department of Wood Science & Technology, College of Agriculture & Life Sciences, Kyungpook National University)
  • 투고 : 2017.01.04
  • 심사 : 2017.01.11
  • 발행 : 2017.01.25

초록

본 연구는 폭쇄처리된 신갈나무로부터 유래된 열수추출물의 안전성 및 항산화성 효과에 대해 검토하였다. 안전성 시험으로는 세포독성실험, 경구투여 독성시험이 실시되었으며, 신갈나무 폭쇄처리 열수추출물의 항산화성 유무를 확인하기 위해서 실험동물은 일본 Air Logistics사의 4주령 B6C3F1 숫컷 생쥐로 다섯 군(1군 10마리)으로 나누어서 실험을 하였다. 신갈나무 폭쇄처리 열수추출물의 세포독성 및 경구투여 독성시험의 결과로 독성은 관찰되지 않았으며, 신갈나무 폭쇄처리 열수추출물의 투여군에서 과산화물분해효소의 활성이 29% 이상 증가하여 과산화물분해효소의 활성이 유의적으로 인정되었다. 따라서 신갈나무 폭쇄처리 열수추출물은 뛰어난 안전성과 항산화성이 있음을 확인하였다.

In this study, natural extract of Ouercus mongolica by steaming explosion treatment were tested for antioxidant activity and safety. To determine the antioxidative properties of the hot water extracts, experiments were carried out by dividing into four groups (10 mice per group) of four-week-old B6C3F1 male mice from Air Logistics, Japan. As safety test, cell viability test, oral toxicity test were done. The activity of peroxidase was increased by more than 29% in the group treated with hydrothermal extract. From 4 kinds of safety tests, toxicity was not observed. From experimental results, natural extract of Ouercus mongolica by steaming explosion treatment showed superb safety and antioxidant effect.

키워드

참고문헌

  1. Cara, C., Ruiz, E., Ballesteros, I., Negro, M.J., Castro, E. 2006. Enhanced enzymatic hydrolysis of olive tree wood by steam exolosion and alkaline peroxide delignification. Process Biochemistry 41: 423-429. https://doi.org/10.1016/j.procbio.2005.07.007
  2. Chung, T.W., Lee, Y.C., Kim, C.H. 2004a. Hepatitis B viral HBx induces matrix metalloproteinase-9 gene expression through activation of ERK and PI-3K/AKT pathways: involvement of invasive potential. FASEB Journal 18(10): 1123-1125. https://doi.org/10.1096/fj.03-1429fje
  3. Chung, T.W., Moon, S.K., Chang, Y.C., Ko, J.H., Lee, Y.C., Cho, G., Kim, S.H., Kim, J.G., Kim, C.H. 2004b. Novel and therapeutic effect of caffeic acid and caffeic acid phenyl ester on hepatocarcinoma cells: complete regression of hepatoma growth and metastasis by dual mechanism. FASEB Journal 18(14): 1670-1681. https://doi.org/10.1096/fj.04-2126com
  4. Cuperus, T., Coorens, M., van Dijk, A., Haagsman, H.P. 2013. Avian host defense peptides, Developmental and Comparative Immunology 41(3): 352-369. https://doi.org/10.1016/j.dci.2013.04.019
  5. Demeule, M., Brossard, M., Page, M., Gingras, D., Beliveau, R. 2000. Matrix metalloproteinase inhibition by green tea catechins. Biochimica et Biophysica Acta 1478: 51-60. https://doi.org/10.1016/S0167-4838(00)00009-1
  6. Ha, K.T., Kim, J.K., Lee, Y.C., Kim, C.H. 2004a. Inhibitory effect of Daesungki-Tang on the invasiveness potential of hepatocellular carcinoma through inhibition of matrix metalloproteinase-2 and -9 activities. Toxicology and Applied Pharmacology 200(1): 1-6. https://doi.org/10.1016/j.taap.2004.03.012
  7. Ha, K.T., Lee, T.K., Kwak, K.H., Kim, J.K., Kim, D.I., Choi, D.Y., Kim, C.H. 2004b. Inhibitory effectof Cho-Deung-San on human aortic smooth muscle cell migration induced by TNF-alpha through inhibition of matrix metalloproteinase-2 and -9 activity. Vascular Pharmacology 41(3): 83-90. https://doi.org/10.1016/j.vph.2004.05.003
  8. Ha, K.T., Kim, J.K., Kang, S.K., Kim, D.W., Lee, Y.C., Kim, H.M., Kim,C.H. 2004c. Inhibitory effect of Sihoga-Yonggol-Moryo-Tang on matrix metalloproteinase-2 and -9 activities and invasiveness potential of hepatocellular carcinoma. Pharmacological Research 50(3): 279-285. https://doi.org/10.1016/j.phrs.2004.02.006
  9. Haas, T.L. 2005. Endothelial cell regulation of matrix metalloproteinases. Canadian Journal of Physiology & Pharmacology 83(1): 1-7. https://doi.org/10.1139/y04-120
  10. Heber, D. 2001. Herbs and atherosclerosis. Current Atherosclerosis Reports: 93-96.
  11. Huang, Y., Wei, X., Zhou, S., Liua, M., Tu, Y., Li, A., Chen, P., Wang, Y., Zhang, X., Tai, H., Peng, L., Xia, T. 2015. Steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in G. barbadense and G. hirsutum. Bioresource Technology 181: 224-230. https://doi.org/10.1016/j.biortech.2015.01.020
  12. Inoue, M., Tajima, K., Hirose, K., Hamajima, N., Takezaki, T., Kuroishi, T., Tominaga. S. 1998. Tea and coffee consumption and the risk of digestive tract cancers: data from a comparative case-referent study in Japan, Cancer Causes Control 9: 209-216. https://doi.org/10.1023/A:1008890529261
  13. International Agency for Research on Cancer. 1991. Coffee, tea, mate, methylxanthines, and methylgloxyal. Lyon, France: IARC Monogor Eval Carcinog Risk Hum 51: 41-271.
  14. Jung, J.Y., Yang, J.K. 2016. Enhancing Enzymatic Digestibility of Miscanthus sinensis using Steam Explosion Coupled with Chemicals. Journal of the Korean Wood Science and Technology 44(2): 218-230. https://doi.org/10.5658/WOOD.2016.44.2.218
  15. Kaegi, E. 1998. Unconventional therapies for cancer: 2. Green tea. The Task Force on Alternative Therapies of the Canadian Breast Cancer Research Initiative. Canadian Medical Association Journal 158: 1033-1035.
  16. Kim, J.H.., Lee, J.S., Lee, K.R., Shim, M.J., Lee, M.W., Shin, P.G., Cheong, J.C., Yoo, Y.B., Lee, T.S. 2012. Immunomodulating and Antitumor Activities of Panellus serotinus Polysaccharides, Mycobiology 40(3): 181-188. https://doi.org/10.5941/MYCO.2012.40.3.181
  17. Kondo, S., Kubota, S., Shimo, T., Nishida, T., Yosimichi, G., Eguchi, T., Sugahara, T., Takigawa, M. 2002. Connective tissue growth factor increased by hypoxia may initiate angiogenesis in collaboration with matrix metalloproteinases. Carcinogenesis 23(5): 769-776. https://doi.org/10.1093/carcin/23.5.769
  18. Lee, M.O., Jang, H.J., Rengaraji, D., Yang, S.-Y., Han, J.Y., Lamont, S.J., Womack, J.E. 2016. Tissue expression and antibacterial activity of host defense peptides in chicken. BMC Veterinary Research 12: 231-240. https://doi.org/10.1186/s12917-016-0866-6
  19. Maeda, K., Kuzuya, M., Cheng, X.W., Asai, T., Kanda, S., Tamaya-Mori, N., Sasaki, T., Shibata, T., Iguchi, A. 2003. Green tea catechins inhibit the cultured smooth muscle cell invasion through the basement barrier. Atherosclerosis 166: 23-30. https://doi.org/10.1016/S0021-9150(02)00302-7
  20. Malemud, C.J. 2006. Matrix metalloproteinases (MMPs) in health and disease: an overview. FRONTIERS IN BIOSCIENCE 11: 1696-1701. https://doi.org/10.2741/1915
  21. Moon, S.K., Cho, G.O., Jung, S.Y., Gal, S.W., Kwon, T.K., Lee, Y.C., Madamanchi, N.R., Kim, C.H. 2003. Quercetin exerts multiple inhibitory effects on vascular smooth muscle cells: role of ERK1/2, cellcycle regulation, and matrix metalloproteinase-9. Biochem. Biophys. Res. Commun. 301: 1069-1078. https://doi.org/10.1016/S0006-291X(03)00091-3
  22. Mosmann. T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  23. Nedamani, E. Ranjbar, Sadeghi Mahoonak, A.R., Ghorbani, M., Kashaninejad, M. 2014. Antioxidant interactions in green tea and oak extracts combination. Journal of Food Science & Technology (2008-8787) 12(49): 121-130.
  24. Olefsky, Jerrold M; Glass, Christopher K, Macrophages. 2009. inflammation, and insulin resistance, Annual Review of Physiology 72: 219-246.
  25. Sheehan, J., Himmel, M.E. 1999. Enzymes, energy, and the environment: Cellulose development in the emerging bioethanol industry. Biotechnology Progress 15: 817-827. https://doi.org/10.1021/bp990110d
  26. Sua, C.-L., Wub, C.-J., Chenc, F.-N., Wangd, B.-J., Sheue, S.-R., Wonf. S.-J. 2007. Supernatant of bacterial fermented soybean induces apoptosis of human hepatocellular carcinoma Hep 3B cells via activation of caspase 8 and mitochondria. Food and Chemical Toxicology 45(2): 303-314. https://doi.org/10.1016/j.fct.2006.07.031
  27. Sun, S., Cao, X., Zhang, X., Xu, F., Sun, R., Jones, G. 2014. Characteristics and enzymatic hydrolysis of cellulose-rich fractions from steam exploded and sequentially alkali delignified bamboo (Phyllostachys pubescens). Bioresource Technology 163: 377-380. https://doi.org/10.1016/j.biortech.2014.04.082
  28. Wang, M., Qin, X., Mudgett, J.S., Ferguson, T.A., Senior, R.M., Welgus, H.G. 1999. Matrix metalloproteinase deficiencies affect contact hypersensitivity: Stromelysin-1 deficiency prevents the response and gelatinase B deficiency prolongs the response, Proceedings of the National Academy of Sciences of the United States of America 96(12): 6885-6889. https://doi.org/10.1073/pnas.96.12.6885