BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Caffeine-induced food-avoidance behavior is mediated by neuroendocrine signals in Caenorhabditis elegans

  • Min, Hyemin (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Youn, Esther (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kawasaki, Ichiro (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Shim, Yhong-Hee (Department of Bioscience and Biotechnology, Konkuk University)
  • 투고 : 2016.07.26
  • 심사 : 2016.09.29
  • 발행 : 2017.01.31
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초록

High-dose caffeine uptake is a developmental stressor and causes food-avoidance behavior (aversion phenotype) in C. elegans, but its mode of action is largely unknown. In this study, we investigated the molecular basis of the caffeine-induced aversion behavior in C. elegans. We found that aversion phenotype induced by 30 mM caffeine was mediated by JNK/MAPK pathway, serotonergic and dopaminergic neuroendocrine signals. In this process, the dopaminergic signaling appears to be the major pathway because the reduced aversion behavior in cat-2 mutants and mutants of JNK/MAPK pathway genes was significantly recovered by pretreatment with dopamine. RNAi depletion of hsp-16.2, a cytosolic chaperone, and cyp-35A family reduced the aversion phenotype, which was further reduced in cat-2 mutants, suggesting that dopaminergic signal is indeed dominantly required for the caffeine-induced food aversion. Our findings suggest that aversion behavior is a defense mechanism for worms to survive under the high-dose caffeine conditions.

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참고문헌

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