Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Lignosulfonic acid promotes hypertrophy in 3T3-L1 cells without increasing lipid content and increases their 2-deoxyglucose uptake

  • Hasegawa, Yasushi (College of Environmental Technology, Muroran Institute of Technology) ;
  • Nakagawa, Erina (College of Environmental Technology, Muroran Institute of Technology) ;
  • Kadota, Yukiya (College of Environmental Technology, Muroran Institute of Technology) ;
  • Kawaminami, Satoshi (College of Environmental Technology, Muroran Institute of Technology)
  • Received : 2016.03.31
  • Accepted : 2016.06.13
  • Published : 2017.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: Adipose tissue plays a key role in the development of obesity and diabetes. We previously reported that lignosulfonic acid suppresses the rise in blood glucose levels through the inhibition of ${\alpha}$-glucosidase activity and intestinal glucose absorption. The purpose of this study is to examine further biological activities of lignosulfonic acid. Methods: In this study, we examined the effect of lignosulfonic acid on differentiation of 3T3-L1 cells. Results: While lignosulfonic acid inhibited proliferation (mitotic clonal expansion) after induction of differentiation, lignosulfonic acid significantly increased the size of accumulated lipid droplets in the cells. Semi-quantitative reverse transcription polymerase chain reaction analysis showed that lignosulfonic acid increased the expression of the adipogenic transcription factor, peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$), leading to increased glucose transporter 4 (Glut-4) expression and 2-deoxyglucose uptake in differentiated 3T3-L1 cells. Additionally, feeding lignosulfonic acid to diabetic KK-Ay mice suppressed increase of blood glucose level. Conclusion: Lignosulfonic acid may be useful as a functional anti-diabetic component of food.

Keywords

References

  1. Fuller S, Beckl E, Salman H, Tapsell L. New horizons for the study of dietary fiber and health: a review. Plant Foods Hum Nutr 2016;71:1-12. https://doi.org/10.1007/s11130-016-0529-6
  2. Barnard DL, Heaton KW. Bile acids and vitamin A absorption in man: the effects of two bile acid-binding agents, cholestyramine and lignin. Gut 1973;14:316-8. https://doi.org/10.1136/gut.14.4.316
  3. Dizhbite T, Telysheva G, Jurkjane V, Viesturs U. Characterization of the radical scavenging activity of lignins-natural antioxidants. Biores Tecnol 2004;95:309-17. https://doi.org/10.1016/j.biortech.2004.02.024
  4. Pouteau C, Dole P, Cathala B, Averous L, Boquillon N. Antioxidant properties of lignin in polypropylene. Polym Degrad Stabil 2003;81:9-18. https://doi.org/10.1016/S0141-3910(03)00057-0
  5. Reddy BS, Maeura Y, Wayman M. Effects of dietary corn bran and autohydrolyzed lignin on 3, 2-dimethyl-4-aminobiphenyl-induced intestinal carcinogenesis in male F344 rats. J Natl Cancer Inst 1983;71:419-23.
  6. Yamamoto Y, Shirono H, Kono K, Ohashi Y. Immunopotentiating activity of the water-soluble lignin rich fraction prepared from LEM-The extract of the solid culture medium of Lentinus encodes mycelia. Biosci Biotechnol Biochem 1997;61:1909-12. https://doi.org/10.1271/bbb.61.1909
  7. Qiu M, Wang Q, Yuan Z, et al. Lignosulfonic acid exhibits broadly anti-HIV-1 activity potential as a microbicide candidate for the prevention of HIV-1 sexual transmission. PLoS One 2012;7:1-12.
  8. Kuschner K. Preparation of large amounts of vanillin from sulfite waste liquors. J Prakt Chem 1928;118:238-64. https://doi.org/10.1002/prac.19281180121
  9. Zhe W, Jingwen X, Jishi Z, Wenxia L. Concrete superplasticizer prepared from catalytic oxidation of lignosulfonate. Adv Mater Res 2012;424:1088-92.
  10. Lehrke M, Lazar MA. The many faces of PPAR${\gamma}$. Cell 2005;123:993-9. https://doi.org/10.1016/j.cell.2005.11.026
  11. Karbowska J, Kochan Z. Role of adiponectin in the regulation of carbohydrate and lipid metabolism role of adiponectin in the regulation of carbohydrate and lipid metabolism. J Physiol Pharmacol 2006;57:103-13.
  12. Bays H, Mandarino L, DeFronzo RA. Role of the adipocyte, free fatty acids, and ectopic fat in pathogenesis of type 2 diabetes mellitus: peroxisomal proliferator-activated receptor agonists provide a rational therapeutic approach. J Clin Endocrinol Metabol 2004;89:463-78. https://doi.org/10.1210/jc.2003-030723
  13. Yang X, Jansson PA, Nagaey I, et al. Evidence of impaired adipogenesis in insulin resistance. Biochem Biophys Res Commun 2004; 317:1045-51. https://doi.org/10.1016/j.bbrc.2004.03.152
  14. Spiegelman BM. PPAR${\gamma}$: adipogenic regulator and thiazolidinedione receptor. Diabetes 1998;47:507-14. https://doi.org/10.2337/diabetes.47.4.507
  15. Tang QQ, Otto TC, Lane MD. CCAAT/enhancer-binding protein ${\beta}$ is required for mitotic clonal expansion during adipogenesis. Proc Natl Acad Sci USA 2003;100:850-5. https://doi.org/10.1073/pnas.0337434100
  16. Qiu Z, Wei Y, Chen N, et al. DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocyte differentiation into adipocytes. J Biol Chem 2001;276:11988-95. https://doi.org/10.1074/jbc.M011729200
  17. Hasegawa Y, Kadota Y, Hasegawa C, Kawaminami S. Lignosulfonic acid-induced inhibition of intestinal glucose absorption. J Nutr Vitaminol 2015;61:449-54. https://doi.org/10.3177/jnsv.61.449
  18. Takahashi K, Hasegawa Y. Glycoproteins isolated from scallop shell inhibit differentiation of 3T3-L1 preadipocyte cells. Fish Sci 2014;80:1301-10. https://doi.org/10.1007/s12562-014-0801-3
  19. Manthorpe M, Fagnani R, Skaper SD, Varon S. An automated colorimetric microassay for neuronotrophic factors. Brain Res 1986;390:191-8. https://doi.org/10.1016/0165-3806(86)90208-7
  20. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680-5. https://doi.org/10.1038/227680a0
  21. Nam M, Lee WH, Bae EJ, Kim SG. Compound C inhibits clonal expansion of preadipocytes by increasing p21 level irrespectively of AMPK inhibition. Arch Biochem Biophys 2008;479:74-81. https://doi.org/10.1016/j.abb.2008.07.029
  22. Kwon JY, Seo SG, Heo YS, et al. Piceatannol, natural polyphenolic stilbene, inhibits adipogenesis via modulation of mitotic clonal expansion and insulin receptor-dependent insulin signaling in early phase of differentiation. J Biol Chem 2012;287:11566-78. https://doi.org/10.1074/jbc.M111.259721
  23. Huang C, Zhang Y, Gong Z, et al. Berbeline inhibits 3T3-L1 adipocyte differentiation through the PPAR${\gamma}$ pathway. Biochem Biophys Res Commun 2006;348:571-8. https://doi.org/10.1016/j.bbrc.2006.07.095
  24. Habinowski SA, Witters LA. The effects of AICAR on adipocyte differentiation of 3T3-L1 cells. Biochem Biophys Res Commun 2001;286:852-6. https://doi.org/10.1006/bbrc.2001.5484
  25. Fumoto T, Yamaguchi T, Hirose F, Osumi T. Orphan nuclear receptor Nur77 accelerates the initial phase of adipocyte differentiation in 3T3-L1 cells by promoting mitotic clonal expansion. J Biochem 2006;159:181-92.
  26. Prusty D, Park BH, Davis KE, Farmer SR. Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator- activated receptor ${\gamma}$ (PPAR${\gamma}$) and C/EBP${\alpha}$ gene expression during the differentiation of 3T3-L1 preadipocytes. J Biol Chem 2002;277:46226-32. https://doi.org/10.1074/jbc.M207776200
  27. Waki H, Park KW, Mitro N, et al. The small molecule harmine is an antidiabetic cell-type-specific regulator of PPAR${\gamma}$ expression. Cell Metabol 2007;5:357-70. https://doi.org/10.1016/j.cmet.2007.03.010

Cited by

  1. Lignins and Their Derivatives with Beneficial Effects on Human Health vol.18, pp.6, 2017, https://doi.org/10.3390/ijms18061219
  2. Lignosulfonic acid attenuates NF-κB activation and intestinal epithelial barrier dysfunction induced by TNF-α/IFN-γ in Caco-2 cells pp.1861-0293, 2017, https://doi.org/10.1007/s11418-017-1167-5
  3. Lignin - An underutilized, renewable and valuable material for food industry vol.60, pp.12, 2017, https://doi.org/10.1080/10408398.2019.1625025
  4. Extraction of lignin and therapeutic applications of lignin-derived compounds. A review vol.18, pp.3, 2017, https://doi.org/10.1007/s10311-020-00981-3
  5. Characterization of a lignin from Crataeva tapia leaves and potential applications in medicinal and cosmetic formulations vol.180, pp.None, 2021, https://doi.org/10.1016/j.ijbiomac.2021.03.077