The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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The Effect of Douchi Hataedock Treatment for Dermatophagoides Farinae-Induced Atopic Dermatitis-like Skin Lesions by Controlling IL-4 Activity

두시 하태독법의 IL-4 활성 조절이 D. farinae 유도 아토피유사피부염 발병 조절에 미치는 효과

  • Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Semyung University) ;
  • Kim, Jae Kyu (Department of Clinical Medicine, School of Korean Medicine, Pusan National University) ;
  • Cheon, Jin Hong (Department of Clinical Medicine, School of Korean Medicine, Pusan National University) ;
  • Kim, Ki Bong (Department of Clinical Medicine, School of Korean Medicine, Pusan National University)
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 김재규 (부산대학교 한의학전문대학원 임상의학부) ;
  • 천진홍 (부산대학교 한의학전문대학원 임상의학부) ;
  • 김기봉 (부산대학교 한의학전문대학원 임상의학부)
  • Received : 2017.01.17
  • Accepted : 2017.02.10
  • Published : 2017.02.28
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Abstract

Objectives Hataedock method is a Korean medical therapy which removes fetal toxin by orally administering herbal decoction to neonates. This study was to observe skin damage and anti-inflammatory effect via regulating IL-4 activity in NC/Nga mice which were induced atopic dermatitis (AD)-like skin lesion by Dermatophagoides (D.) farinae after applying Douchi Hataedock method. Methods NC/Nga mice with 3 weeks of gestational age were used. Each 10 mice were allocated to the control group (Ctrl), the AD-induced group (AE), and the group which induced AD after administering Douchi extract (GT). After 4 weeks from administering Douchi extract to the mice, the primary AD was induced by applying D. farinae extract 6 times per week for 3 weeks and then the secondary AD was induced by the same method after 1 week from the primary AD induction. To identify the skin damage and anti-inflammatory effect, we observed LxR, IL-4, Fc ${\varepsilon}$ receptor, substance P, and $NF-{\kappa}B$. Results The GT group showed alleviation of skin injury and decrease in capillary angiogenesis. Stratum corneum damage, epithelial cell hyperplasia, lymphocyte infiltration, and capillary distribution relatively decreased in the GT group. LxR-positive reaction in the GT group were increased by 53% than that of the AE group. IL-4 production, $Fc{\varepsilon}$ receptor activity, and substance P-positive reaction in the GT group were decreased by 82%, 42%, and 82% respectively compare to those of the AE group. $NF-{\kappa}B$-positive reaction in the GT group were decreased by 15% compare to that of the AE group. Conclusions Hataedock method with Douchi extract alleviated AD via reducing inflammatory cytokines secreted at the early stage of AD. Thus, Douchi Hataedock method has a beneficial effect for the prevention and treatment of AD.

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References

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