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Anti-bacteria effect of green tea, mulberry, and mate leaves extracts on S. mutans

녹차잎, 뽕잎, 마테잎 추출물이 S. mutans에 미치는 항균효과

  • Kim, Sung-Sook (Dept. of Dentistry, Konyang University Hospital) ;
  • Won, Ji-Hye (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Lee, Go-Eun (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Lee, Ru-Ri (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Lee, Sun-Mi (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Lee, Jin-Hee (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Kang, Kyung-hee (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University)
  • 김성숙 (건양대학교병원 치과) ;
  • 원지혜 (건양대학교 의과학대학 치위생학과) ;
  • 이고은 (건양대학교 의과학대학 치위생학과) ;
  • 이루리 (건양대학교 의과학대학 치위생학과) ;
  • 이선미 (건양대학교 의과학대학 치위생학과) ;
  • 이진희 (건양대학교 의과학대학 치위생학과) ;
  • 강경희 (건양대학교 의과학대학 치위생학과)
  • Received : 2016.11.01
  • Accepted : 2017.01.20
  • Published : 2017.01.28

Abstract

This study aims to figure out how green tea leaves, mulberry leaves, and mate leaves that are often taken around in drinking tea influence on S. mutans known as dental caries causative bacteria. After vaccinating 1% of S. mutans on the extract added badge and incubating 6 hours and 10 hours in $37^{\circ}C$, it calculated absorbance and bacterial colony number. Extract of green tea leaves and mate leaves were added with 0, 0.5, 1.0, 2.0, and 4.0% of concentration. As a result of growth suppressive effect of S. mutans, it may be observed that colony number significantly decreases in higher concentration. When it calculated bacterial colony number with adding 2% extract after 10 hours, 99.0% for green tea leaves, 97.1% for mulberry leaves, and 89.6% for mate leaves appeared.

본 연구는 일상에서 흔히 차로 음용되고 있는 녹차잎, 마테잎, 뽕잎이 치아우식원인균으로 알려진 S. mutans에 미치는 항균효과를 알아보고자 하였다. S. mutans를 추출물이 첨가된 배지에 1%씩 접종하고 $37^{\circ}C$에서 6시간과 10시간동안 배양한 후 흡광도 및 세균 집락수를 측정하였다. 녹차잎, 마테잎, 추출물은 0, 0.5, 1.0, 2.0, 4.0%의 농도로 배지에 첨가하였다. S. mutans의 성장억제효과를 확인한 결과, 농도가 높을수록 colony의 수가 현저히 줄어드는 것을 관찰 할 수 있었다. 2% 추출물을 첨가하고 10시간 후 세균 집락수를 측정하였을 때 녹차잎은 99.0%, 뽕잎은 97.1%, 마테잎에서는 89.6%의 높은 성장억제율을 나타내었다.

Keywords

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