Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Subchronic Oral Toxicity Study of Acanthopanax divaricatus var. albeofructus in Rats

  • Kim, Myoung Jun (Department of Pathology, Biotoxtech Co., Ltd.) ;
  • Lee, Mi Ju (Department of Pathology, Chronic Inhalation Toxicity Research Center, Chemicals Toxicity Research Bureau, Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency) ;
  • Lee, Yong-Hoon (Department of Pathology, Chronic Inhalation Toxicity Research Center, Chemicals Toxicity Research Bureau, Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency) ;
  • Park, Sun Hee (Department of Pathology, Biotoxtech Co., Ltd.) ;
  • Kim, Duyeol (Department of Pathology, Biotoxtech Co., Ltd.) ;
  • Park, Cheol Beom (Department of Pathology, Biotoxtech Co., Ltd.) ;
  • Kang, Jin Seok (Department of Biomedical Laboratory Science, Namseoul University) ;
  • Kang, Jong-Koo (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chungbuk National University)
  • Received : 2016.06.30
  • Accepted : 2016.12.09
  • Published : 2017.01.15
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Abstract

Acanthopanax divaricatus (Siebold & Zucc.) Seem. var. albeofructus (ADA), a traditional medical herb, has been used to treat arthritis and muscular injury, to strengthen muscle and bone, and to get vital energy. However, information regarding its toxicity is limited. ADA was administered by oral gavage to groups of rats at doses of 0 (control), 1,000, 1,500, 2,000, 2,500, and 3,000 mg/kg five times per week for 13 weeks. Mortality, clinical signs, body weights, food consumption, hematology, serum chemistry, urinalysis, organ weights, necropsy, histopathological finding, vaginal cytology, and sperm morphology were compared between control and ADA-treated groups. Salivation was intermittently observed in both sexes receiving 2,500 and 3,000 mg/kg directly after dosing. Absolute liver weights increased in females receiving 2,000, 2,500, and 3,000 mg/kg ADA (P < 0.05, P < 0.01, and P < 0.01, respectively) and so did the relative liver weights (P < 0.001). Salivation and increased liver weight were ADA-related changes but not considered to be adverse effects. Salivation was intermittent and transient, and the liver weight increase was minor and not accompanied by other changes such as hepatic morphological or functional alterations. The no-observed-adverse-effect-level was determined to be at least 3,000 mg/kg in both sexes of rats.

Keywords

References

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