한국융합학회논문지 (Journal of the Korea Convergence Society)

  • 제8권1호
  • /
  • Pages.35-42
  • /
  • 2017
  • /
  • 2233-4890(pISSN)
  • /
  • 2713-6353(eISSN)
한국융합학회 (Korea Convergence Society)
권호 표지
DOI QR코드

DOI QR Code

경피 침투율이 높은 보름달 물해파리 유래 바이오 융합 소재 개발

Development of bio-fusion materials with skin penetrating property derived from Aurelia aurita

  • 투고 : 2016.10.20
  • 심사 : 2017.01.20
  • 발행 : 2017.01.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

보름달 물해파리 유래 펩타이드인 LVH는 주름개선에 효과가 있는 기능성 소재로서 보고되어 있다. 본 연구에서는 LVH의 피부 침투 효율을 증가시키기 위하여 LVH와 팔미트산을 이용하여 pal-LVH를 합성하였고, 합성된 pal-LVH의 기능성 소재로서의 효과를 조사하였다. 이를 위해서 우리는 두 펩타이드의 세포독성, 항노화, 주름 개선 그리고 피부 자극에 대한 효능을 비교 평가하였다. Pal-LVH는 LVH와 마찬가지로 세포독성은 보이지 않았다. 그리고 고농도에서는 LVH와 비슷한 우수한 상처 치유와 항노화 능력을 보였고, 주름개선 효과 또한 저농도에서는 LVH와 거의 유사하게 나타났다. 이들 결과는 pal-LVH 펩타이드는 피부 침투율을 증가시키면서, LVH 펩타이드가 가지고 있는 우수한 효능에는 영향을 주지 않는다는 것을 지시한다. 따라서 pal-LVH는 주름개선 효과 및 안티에이징 물질 등의 기능성 신소재로서 가능성을 제시하며, 다양한 융합 연구를 통하여 의약품 개발에 응용되어 질 수 있다.

Previously, we reported LVH peptiede derived from Aurelia aurita as cosmecetuticals with anti-winkle activity. In this study, we synthesized pal-LVH using palmitic acid to enhance skin permeability of LVH and examined the effects as cosmeceuticals of pal-LVH. To evaluate these effects, we performed cell toxicity, wound healing, and patch test for skin irritation with LVH and pal-LVH and compared these results for their effects. As a result. pal-LVH was not showed in cytotoxicity and allergenic effect like as LVH. Besides, pal-LVH had almost same excellent anti-ageing properties in high concentration and anti-winkle effect in low concentrationwas as LVH. These results suggested synthesis of palimitic acid and LVH did not affect any functions as cosmeceuticals with increasing skin permeability. Therefore, pal-LVH can be adaptable as new cosmecetuticals with anti-winkle and anti-ageing materials and applied in the development of medicine through various convergence study.

키워드

참고문헌

  1. "The report of Chungnam national marine bio industry Promotion Plan", 2010.
  2. C. E. Mills, "Jellyfish blooms: are populations increasing globally in response to changing ocean conditions?", Hydrobiologia, Vol. 451, No. 1, pp. 55-68, 2001. https://doi.org/10.1023/A:1011888006302
  3. "Study on the causes of and countermeasure against jellyfish bloom", pp. 222-2008, 2013
  4. W. B. Yoo, "Study on the rDNA characteristics of harmful jellyfishes Aurelia sp.1 and Nemopilema nomurai in Korea", Ph.D. dissertation, Sangmyung university, 2016.
  5. Y-H. Peggy, HsiehFui-Ming, LeongJack, Rudloe, "Jellyfish as food", Hydrobiologia, Vol. 451, No. 1, pp. 11-17, 2001. https://doi.org/10.1023/A:1011875720415
  6. Y. MORINAGA, K. IWAI, H. TOMITA, Y. TAKAYA, T. NARAOKA, & H. MATSUE, "Chemical Nature of a New Antihypertensive Peptide Derived from Jellyfish TI", Food Science and Technology Research, Vol. 16, No. 1, pp. 333-340, 2010. https://doi.org/10.3136/fstr.16.333
  7. Y. Zhuang, H. Hou, X. Zhao, Z. Zhang, B. Li, "Effects of Collagen and Collagen Hydrolysate from Jellyfish (Rhopilema esculentum) on Mice Skin Photoaging Induced by UV Irradiation", Journal of Food Science, Vol. 74, pp. 183-188, 2009. https://doi.org/10.1111/j.1750-3841.2009.01236.x
  8. S. H. Moh "Development of cosmeceutical Derived from Aurelia aurita", Small and Medium Business Administration, 2013.
  9. H. H. Seo, M. J. Cho, J. H. Lee, J. H. Lee, S. J. Kim, G. S. Lee, T. K Lee, S. H. Moh, "Anti-aging effects from extracts of Aurelia aurita", The Korea Academia-Industrial cooperation Society Semiannual, Vol. 1, pp. 274-277, 2012.
  10. H. S. Kim, B. K. Kang, M. S Lee, T. W. Kim, "Research Trends of Drug Delivery Stystem for Peptide Drug", Tissue Engineering and Regenerative Medicine, Vol. 5, No. 1, pp. 61-69, 2008.
  11. G. U. Choi, "Characterization of a palmitoylated NF-${\kappa}B$ specific inhibitor peptide for its skin penetration, anti-inflammation and anti-aging efficacy", Ph.D. dissertation, Dongguk university, 2014.
  12. W. Lu, M. A. Qasim, S. B. H. Kent, "Comparative total syntheses of turkey ovomucoid third domain by both stepwise solide phase peptide synthesis and native chemical ligation",. J. Am. Chem. Soc., Vol. 118, pp. 8518-8523, 1996. https://doi.org/10.1021/ja960812o
  13. Dirk T.S. Rijkers, John A.W. Kruijtzer,, J. Antoinette Killian, Rob M.J. Liskamp, "A convenient solid phase synthesis of S-palmitoyl transmembrane peptides", Tetrahedron Letters, Vol. 46, pp. 3341-3345, 2005 https://doi.org/10.1016/j.tetlet.2005.03.079
  14. J. van Meerloo, G. J. Kaspers, J. Cloos, "Cell sensitivity assays: the MTT assay", Methods Mol Biol., Vol. 731, pp. 237-245, 2011.
  15. M. S. Blois,"Antioxidantdeterminationbytheuse a stable free radical", Nature, Vol. 26, pp. 199-263, 1958.
  16. H. J. Kim, "Convergence study on the antioxidant effect of crude extracts of Nelumbo nucifera Gaertner", Journal of the Korea Convergence Society, Vol. 7. No. 3, pp. 53-58, 2016. https://doi.org/10.15207/JKCS.2016.7.3.053
  17. C. C. Liang, A. Y. Park, J. L. Guan, "In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro", Nature Protocols, Vol. 2, pp. 329-333, 2007. https://doi.org/10.1038/nprot.2007.30
  18. M. D. Abramoff, P. J. Magalhaes, S. J. Ram, "Image Processing with ImageJ", Biophotonics International, Vol. 11, No. 7, pp. 36-42, 2004.
  19. "Guidelines on in vivo tests and in vitro tests of cosmetic products", Korea food and drug adminstration, 2011.