Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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A Permeation Characteristics Study of Water- or Oil-soluble Substances through Condition Setting for the In Vitro Skin Absorption Method

피부흡수 대체시험법의 조건설정을 통한 수용성, 지용성 물질의 투과 특성 연구

  • Seo, Ji-Eun (Department of Public Health, College of Natural Sciences, Keimyung University) ;
  • Lee, Jinho (Department of Chemistry, College of Natural Sciences, Keimyung University) ;
  • Kim, Bae-Hwan (Department of Public Health, College of Natural Sciences, Keimyung University)
  • 서지은 (계명대학교 자연과학대학 공중보건학전공) ;
  • 이진호 (계명대학교 자연과학대학 화학전공) ;
  • 김배환 (계명대학교 자연과학대학 공중보건학전공)
  • Received : 2016.12.21
  • Accepted : 2017.02.20
  • Published : 2017.02.28
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Abstract

Objectives: The purpose of this study was to compare permeation characteristics in three skin types using oil-soluble benzoic acid and water-soluble caffeine after method condition optimization based on OECD guideline 428. Methods: A Franz diffusion cell, a reliable alternative method for skin permeation, was used. One-milliliter samples were taken and immediately replaced with fresh solution in the receptor chamber at regular time intervals (1, 2, 4, 7, 10 and 24 hr). The amount of test substances was measured by LC-MS/MS. Results: The permeation rate increased dose-dependently, and the permeation orders were $KeraSkin^{TM}$ > hairless mouse full skin > human cadaver epidermis for skin types, and benzoic acid solution > caffeine solution > benzoic acid cream > caffeine cream for type of test materials. Conclusion: According to the definitions of Marzulli, benzoic acid and caffeine would be classified as 'fast' and 'moderate' compared with the permeation of other chemical species. The setting conditions and permeation characteristics performed in this study are expected to contribute to future permeation studies.

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References

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