Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지
DOI QR코드

DOI QR Code

Establishment and Selection of Indicator Materials for Cervi Parvum Cornu Pharmacopuncture

  • Received : 2017.07.10
  • Accepted : 2017.08.22
  • Published : 2017.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Recently, Cervi Parvum Cornu pharmacopuncture has been widely used. But no studies on the indicator materials for Cervi Parvum Cornu pharmacopuncture have been conducted. The aim of this study was to select indicator materials that would aid in the uniform preparation of standardized Cervi Parvum Cornu pharmacopuncture. Methods: Three lots of Cervi Parvum Cornu pharmacopuncture were analysed. Each lot was prepared using the same methods and materials. Chondroitin sulfate, alanine, and leucine were selected as the indicator materials for Cervi Parvum Cornu. For standardization, chondroitin sulfate analysis was performed using the colorimetric method, while alanine and leucine were analyzed using liquid chromatography-mass spectrometry (LC-MS). Results: Analysis of the three lots of Cervi Parvum Cornu pharmacopuncture found chondroitin sulfate levels of $108.9{\pm}17.3ug/ml$, $118.8{\pm}5.0ug/ml$ and $112.3{\pm}11.9ug/ml$. Alanine levels were $44.9{\pm}2.8ug/ml$, $44.6{\pm}0.3ug/ml$, and $43.9{\pm}0.2ug/ml$. Leucine levels were $29.6{\pm}0.7ug/ml$, $29.0{\pm}0.1ug/ml$, and $29.4{\pm}0.1ug/ml$. Conclusion: These results suggest that chondroitin sulfate, alanine, and leucine may be useful for the standardization of Cervi Parvum Cornu pharmacopuncture.

Keywords

References

  1. Moreau M, Dupuis J, Bonneau NH, Lecuyer M. Clinical evaluation of a powder of quality elk velvet antler for the treatment of osteoarthrosis in dogs. Can Vet J 2004;45:133-139.
  2. Mikler JR, Theoret CL, High JC. Effects of topical elk velvet antler on cutaneous wound healing in streptozotocin induced diabetic rats. J Altern Complement Med 2004;10:835-840. https://doi.org/10.1089/acm.2004.10.835
  3. You L, Zhao M, Regenstein JM, Ren J. In vitro antioxidant activity and in vivo anti-fatigue effect of loach (Misgurnusanguillicaudatus) peptides prepared by papain digestion. Food Chem 2011;124:188-194. https://doi.org/10.1016/j.foodchem.2010.06.007
  4. Lee HJ, Cho HS, Hwang MS, Jung CY, Lee DG, Kim EJ, et al. Effect of Cervi Pantotrichum Cornu pharmacopuncture on suppressing the expression of iNOS and production of NO in type-II collagen induced arthritis mice. The Acupuncture 2008;25:105-116.
  5. Chung YR, Lee SD, Byun H, Park IS, Jung CY, Lee CH, et al. The effect of deer antler herbal acupuncture control to hyper-inflammatory responses on synovial membrane by LPS-induced arthritis. The Acupuncture 2007;24:167-181.
  6. Kim EJ, Kim GY, Chung HW. Effect of Achyrantis Radix administration and Cervi Cornu Parvum acupuncture in experimental osteoarthritis rats. Korean J Oriental Physiol Pathol 2007;21:1194-1199.
  7. Ahn HJ, Kim KS. Inhibitory effect of deer antler aqua-acupuncture (DAA) on cathepsin S activity and rheumatoid arthritis in rats. The Acupuncture 2003;20:104-116.
  8. Kim JG, Kim KS. Inhibitory effects of Cervi Pantotrichum Cornu herbal acupuncture on type-II collagen-induced arthritis. The Acupuncture 2002;19:155-170.
  9. Choi YH, Choi WS, Song IK, Park JS, Lee SD, Kim KS. Protective and anti-arthritic effects of Cervi Pantotrichum Cornu herbal acupuncture, inhibiting dihydroorotate dehydrogenase, on phosphate-ion-mediated chondrocyte apoptosis and rat collagen-induced arthritis. The Acupuncture 2002;19:10-27.
  10. Choi BJ, Kim MJ, Park SD, Lee AR, Jang JH, Kim KH. Modulation of bone mass, strength and turnover by a Cervi Pantotrichum Cornu herbal acupuncture in adjuvant-induced arthritic rats. The Acupuncture 2002;19:219-233.
  11. Park SD, Kim MJ, Lee AR, Jang JH, Kim KH. Effect of Cervi Pantotrichum Cornu herbal acupuncture on protease activities, antioxidant in rheumatoid arthritis rats. The Acupuncture 2002;19:51-64.
  12. Hwang JS, Hwang JH, Lee HJ, Lee DG, Kang MJ, Back SO, et al. The ability of Cervus Elaphus Sibiricus herbal acupuncture to inhibit the generation of inflammatory enzymes on collagen induced arthritis mice. The Acupuncture 2007;24:1-14.
  13. Kim JK, Lee SD, Jeong YR, Kim KS. Protective action of cartilage and bone destruction by deer antler herbal-acupuncture solution, the pilose antler of Cervus Korean TEMMINCK Var. Mantchuricus Swinhoe, on Type-II collagen-induced arthritis in mice. The Acupuncture 2006;23:73-90.
  14. Kim WY, Lee SD, Kim KH, Baek ST, Kim KS. The efficiency of deer antler herbal acupuncture on modulation and prevention of IL-1 mediated activation in rat chondrocytes at a receptor level. The Acupuncture 2006;23:113-123.
  15. Kim JK, Kim KS. Effects of the pilose antler of Cervus Korean TEMMINCK var. mantchuricus Swinhoe (DAS), herbal acupuncture solution on suppression of collagenolysis and bone resorption in mouse calvarial osteoblasts. The Acupuncture 2004;21:225-236.
  16. Kim MJ, Lee SD, Kim KH, Byun H, Kim KS. Effects of deer antler water extract (pilose antler of Cervus Korean TEMMINCK var. mantchuricus Sinhoe) on chondrocytes. The Acupuncture 2006;23:103-111.
  17. Han SW, Lee YH, Kim CH. A study on effects of the Cervi Pantotricuhum Cornu herb-acupuncture on the osteoporosis induced by ovariectomy in rats. J Pharmacopuncture 2000;3:177-191. https://doi.org/10.3831/KPI.2000.3.1.177
  18. Yook TH, Lee CH, Lee HI. A study on the effects of the Carthamisemen, Cervi Pantotrichum Cornu, Hominis placenta aquacupuncture on the osteoporosis in rats. The Acupuncture 2001;18:61-75.
  19. Han SW, Choi JY, Lee YH. Healing of bony defects by Cervi Pantotricuhum Cornu herbal acupuncture. The Acupuncture 2001;18:135-146.
  20. Lee KB, Park SK. Effects of Cornu Cervi Parvum pharmacopuncture on the blood picture and antioxidative activity in rats. Korean J Acupunct 2010;27:25-34.
  21. Lee JH, Lee KM, Kim JS. Anti-wrinkle effects of Cervi Pantotrichum Cornu pharmacopuncture solution. The Acupuncture 2010;27:1-8.
  22. Lee JM, Kim YT, Lee HI, Son YS, Jin SH, Lee HS, et al. The effects of Cervus elaphus aquacupuncture and Gingseng radix aquacupuncture on the growth of animals. J Pharmacopuncture 2000;3:131-152. https://doi.org/10.3831/KPI.2000.3.2.131
  23. Kim YT, Son YS, Jin SH, Han SW, Shim IS, Lim SBN, et al. The effects of Cervus elaphus on the growth and the intellectual development of animals. The Acupuncture 2001;18:122-134.
  24. Kwak DG, Yang CH. A regulatory effect of Cervi Cornu Parvum aquaacupuncture on serum estradiol level after ovariectomy. The Acupuncture 1998;15:29-41.
  25. Lee HY, Lee JB, Cho YH, Song BY, Yook TH. The effects of Cervi Pantotrichum Cornu pharmacopuncture and Bovis Calculus.FelUrsi pharmacopuncture on the heart rate variability. The Acupuncture 2010;27:65-74.
  26. Kim HJ, Song BY, Yook TH. The effects of distilled Cervi Pantotrichum Cornu pharmacopuncture and Zzizyphispinosi semen pharmacopuncture on the heart rate variability. J Pharmacopuncture 2009;12:31-40.
  27. Ryu SH, Lee KM, Lee BH, Lim SC, Jun TY, Seo JC. Oligonucleotide chip analysis of Cervi Parvum Cornu herbal-acupuncture solution (CPC-HAS) on SNU484 carcinoma cells. Korean J Acupunct 2006;23:137-148.
  28. Ryu SH, Lee KM, Lee BH, Lim SC, Jung TY, Seo JC. DNA and proteomic expression of Cervi Parvum Cornu herbal-acupuncture solution (CPC-HAS) in HepG2 carcinoma cells. J Pharmacopuncture 2006;9:5-16.
  29. Kim YW, Moon JY, Lim JK, Park WH, Park SD, Nam KS. Effects of CCP (Cervi Cornu Parvum) aqua-acupuncture on glucocorticoid-Induced immunosuppression in murine lymphocyte. The Acupuncture 1997;14:245-252.
  30. Jo SJ, Kim JH, Kim JW, Choi HO, Lee SH, Kim MK, et al. Comparative studies on velvet deer antler and ossified deer antler on the contents of bioactive components and on the bone mineral density improving activity for oophorectomized rat. Nat Prod Sci 2013;19:303-310.
  31. Lee BY, Lee OH, Choi HS. Analysis of food components of Korean deer antler parts. Korean J Food Sci Technol 2003;35:52-56.
  32. Korea Food and Drug Administration. Health Functional Food Act. 2008;3:6-10.
  33. Park SW, Lee JW, Lee JH, Ha IH, Byun JH, Jung BH, et al. Identification of standard compound of Ja-ha-guh pharmacopuncture and validation of analytic methods. JORM 2016;26:33-40.
  34. Lu L, Wang K, Li L, Xuan Z, Gong X. Effect of velvet antler polypeptide on peripheral nerve regeneration. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2008;22:1458-1461. [In Chinese]
  35. Clark DE, Lord EA, Suttie JM. Expression of VEGF and pleiotrophin in deer antler. Anat Rec A Discov Mol Cell Evol Biol 2006;288:1281-1293.
  36. Barling PM, Lai AK, Nicholson LF. Distribution of EGF and its receptor in growing red deer antler. Cell Biol Int 2005;29:229-236. https://doi.org/10.1016/j.cellbi.2004.12.004
  37. Je JY, Park PJ, Lim DH, Jeon BT, Kho KH, Ahn CB. Antioxidant, antiacetylcholinesterase and composition of biochemical components of Russian deer velvet antler extracts. Korean J Food Sci Anim Resour 2011;31:349-355. https://doi.org/10.5851/kosfa.2011.31.3.349
  38. Ivankina NF, Isay SV, Busarova NG, Mischenko TYa. Prostaglandin-like activity, fatty-acid and phospholipid composition of sika deer (Cervus nippon) antlers at different growth stages. Comp Biochem Physiol B 1993;106:159-162. https://doi.org/10.1016/0305-0491(93)90022-W