미생물학회지 (Korean Journal of Microbiology)

  • 제53권4호
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  • Pages.292-296
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  • 2017
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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분열효모에서 THO 복합체의 구성요소인 Tex1/THOC3가 생장 및 mRNA 방출에 미치는 영향

Effect of Tex1/THOC3, a component of THO complex, on growth and mRNA export in fission yeast

  • 배수정 (성신여자대학교 생명과학.화학부) ;
  • 고은진 (성신여자대학교 생명과학.화학부) ;
  • 윤진호 (성신여자대학교 생명과학.화학부)
  • Bae, Soo Jeong (School of Biological Science and Chemistry, Sungshin Women's University) ;
  • Koh, Eun-Jin (School of Biological Science and Chemistry, Sungshin Women's University) ;
  • Yoon, Jin Ho (School of Biological Science and Chemistry, Sungshin Women's University)
  • 투고 : 2017.11.22
  • 심사 : 2017.12.12
  • 발행 : 2017.12.31
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⟨ 이전 논문 다음 논문 ⟩

초록

진핵생물에서 THO/TREX 복합체는 전사 신장, pre-mRNA 가공 및 mRNA의 핵에서 방출에 중요한 역할을 담당한다. 이 복합체는 진화적으로 잘 보존되어 있지만, 생명체에 따라 구성성분과 기능에 차이가 존재한다. 이 논문에서는 출아효모보다는 고등생물과 더 유사한 분열효모 Schizosaccharomyces pombe에서 THO/TREX 복합체의 한 구성요소인 spTex1가 생장과 mRNA의 방출에 필수적이지 않다는 것을 보였다. spTex1 유전자의 결실과 과발현 어느 것도 생장의 결함과 $poly(A)^+$ RNA가 핵 안에 축적되는 현상을 거의 보이지 않았다. 또한 spTex1-GFP 단백질은 주로 핵 안에 위치하였다. Yeast two-hybrid와 Co-immunoprecipitation 분석에서 S. pombe Tex1은 THO/TREX 복합체의 주요 구성인자인 spHpr1 (THOC1), spTho2 (THOC2)과 상호작용을 하였다. 이와 같은 결과들은 S. pombe의 Tex1도 THO/TREX 복합체의 구성인자이지만, 생장과 mRNA 방출에는 중요한 역할을 하지 않음을 의미한다.

In eukaryote, THO/TREX complex plays a critical role in transcriptional elongation, pre-mRNA processing, and nuclear mRNA export. This complex is evolutionally well- conserved, but there are some differences in composition and function according to organisms. Here we showed that spTex1, a component of THO/TREX complex, is not essential for growth and mRNA export in a fission yeast, Schizosaccharomyces pombe, which is more similar to higher eukaryote than budding yeast. Deletion and overexpression of the spTex1 gene do not lead to any detectable growth phenotype and accumulation of poly(A)+ RNA in the nucleus. And the spTex1-GFP protein is localized mainly in the nucleus. Yeast two-hybrid and Co-immunoprecipitation analysis showed that the spTex1 protein interacted with spHpr1 (THOC1) and spTho2 (THOC2), main subunits of THO complex. We conclude that the S. pombe Tex1 is a component of THO/TREX complex, but does not plays important roles in growth and bulk mRNA export from the nucleus.

키워드

참고문헌

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