Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Anti-melanogenesis Effect of Ganoderma lucidum Mycelial Extract Supplemented with Oriental Raisin Tree (Hovenia dulcis) Extract

헛개나무 추출물이 첨가된 영지버섯균사 추출물의 멜라닌 생성 억제효과

  • Kim, Hong Il (Department of Biomedical Chemistry, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University) ;
  • Jeong, Yong Un (Department of Biomedical Chemistry, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University) ;
  • Kim, Jong Hyun (Department of Biomedical Chemistry, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University) ;
  • Choi, In Ho (Department of Biomedical Chemistry, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University) ;
  • Lee, Jun Ho (Department of Biomedical Chemistry, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University) ;
  • Lee, Chang Soo (Department of Biomedical Chemistry, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University) ;
  • Park, Young Jin (Department of Biomedical Chemistry, Research Institute for Biomedical & Health Science, College of Biomedical and Health Science, Konkuk University)
  • 김홍일 (건국대학교 의료생명대학 의생명화학과, 의료생명연구소) ;
  • 정용운 (건국대학교 의료생명대학 의생명화학과, 의료생명연구소) ;
  • 김종현 (건국대학교 의료생명대학 의생명화학과, 의료생명연구소) ;
  • 최인호 (건국대학교 의료생명대학 의생명화학과, 의료생명연구소) ;
  • 이준호 (건국대학교 의료생명대학 의생명화학과, 의료생명연구소) ;
  • 이창수 (건국대학교 의료생명대학 의생명화학과, 의료생명연구소) ;
  • 박영진 (건국대학교 의료생명대학 의생명화학과, 의료생명연구소)
  • Received : 2017.11.13
  • Accepted : 2017.11.30
  • Published : 2017.12.30
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Abstract

The aim of this study is to investigate the effect of Hovenia dulcis (oriental raisin tree) extract on ganodermanondiol (GN) contents in Ganoderma lucidum (G. lucidum) mycelia. GN has a triterpenoid structure and is one of the major active components of G. lucidum. Furthermore, we previously proved its inhibitory effects on tyrosinase activity and melanin biosynthesis in B16F10 melanoma cells. In this study, we observed significantly increased GN contents in G. lucidum mycelial extracts supplemented with 15% (v/v) oriental raisin tree extract (ORTE) by HPLC analysis. In addition, melanogenesis was significantly inhibited by G. lucidum extract supplemented with 15% ORTE when compared to G. lucidum extract without ORTE supplementation. Furthermore, mycelial growth of G. lucidum was increased by ORTE supplementation in both solid and liquid cultivation. These results suggest that the oriental raisin tree is useful as natural ingredient for increasing GN biosynthesis as well as whitening effect of G. lucidum.

본 연구에서는 영지버섯 균사 배양 시 헛개나무 추출물의 첨가가 영지버섯의 가나도마난디올의 생합성에 미치는 영향을 분석하기 위하여 수행하였다. 가나도마난디올은 트리터페노이드 계열의 물질이며 영지버섯의 주요한 생리효능을 가지는 물질 중의 하나이다. 이와 관련하여, 본 연구자들은 선행연구를 통하여 가나도마난디올이 B16F10 멜라노마 세포의 티로시나제 저해 활성 및 멜라닌 생합성 저해능에 우수한 효과가 있는 것을 확인하였다. 본 연구에서 영지버섯 균사 배양 시 15% (v/v)의 헛개나무 추출물 첨가하면 영지버섯의 가나도마난디올 생합성이 첨가하지 않은 대조군에 비해 유의적으로 증가함을 HPLC분석을 통하여 확인하였다. 또한, 15%(v/v)의 헛개나무 추출물을 첨가한 영지균사 배양추출물의 B16F10 멜라노마 세포에 대한 멜라닌 생합성 억제능이 첨가하지 않은 대조군에 비해 유의적으로 증가함을 확인하였다. 또한, 영지버섯 균사 배양 시 헛개나무 추출물 첨가가 미백활성을 가지는 가나도마난디올 생합성 증가뿐 아니라, 액체 및 고체 배양시 균사의 생장도 촉진하는 것을 확인하였다. 이러한 결과들은 헛개나무가 영지버섯균사의 미백활성 물질인 가나도마난디올 생합성의 증가를 유도하고 이로 인한 영지버섯 균사의 미백 활성이 증가하는 유용한 소재로 사용될 수 있다는 것을 시사한다.

Keywords

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