Parasites, Hosts and Diseases

  • 제55권6호
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  • Pages.623-630
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  • 2017
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Development of Monoclonal Antibodies for Diagnosis of Plasmodium vivax

  • Linh, Nguyen Thi Phuong (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University) ;
  • Park, Hyun (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University) ;
  • Lee, Jinyoung (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University) ;
  • Liu, Dong-Xu (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University) ;
  • Seo, Ga-Eun (Department of Microbiology, Ajou University School of Medicine, and Department of Biomedical Science, Graduate School of Ajou University) ;
  • Sohn, Hae-Jin (Department of Microbiology, Ajou University School of Medicine, and Department of Biomedical Science, Graduate School of Ajou University) ;
  • Han, Jin-Hee (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Han, Eun-Taek (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Shin, Ho-Joon (Department of Microbiology, Ajou University School of Medicine, and Department of Biomedical Science, Graduate School of Ajou University) ;
  • Yeo, Seon-Ju (Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University)
  • 투고 : 2017.07.12
  • 심사 : 2017.10.16
  • 발행 : 2017.12.31
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초록

Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.

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참고문헌

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피인용 문헌

  1. Plasmodium falciparum Parasitemia and Band Sensitivity of the SD Bioline Malaria Ag P.f/Pan Rapid Diagnostic Test in Madagascar vol.100, pp.5, 2019, https://doi.org/10.4269/ajtmh.18-1013