DOI QR코드

DOI QR Code

Visualization on the Functional Changes of Endothelial Cells Due to Apoptotic Macrophage in Atherosclerosis Microenvironment

동맥경화의 미세환경에서 대식세포의 사멸에 의한 혈관세포의 기능적 변화에 대한 가시화

  • Received : 2017.11.28
  • Accepted : 2017.12.19
  • Published : 2017.12.31

Abstract

The apoptosis of macrophages occurs throughout all stages of atherosclerosis. It is known to constitute atheromatous plaque, increase plaque instability, and thus contribute to the development of atherosclerosis. However, there still remains much to be elucidated on how the apoptotic macrophages affect the endothelial cells and also how they contribute to the development of atherosclerosis. Here we present a microfluidic system, which enables co-culture of apoptotic macrophages and endothelial cells in fibrin gel that mimics in vivo extracellular matrix. With the system, we can investigate the effect of macrophage apoptosis on vascular endothelial cells by quantitatively analyzing the level of reactive oxygen species of HUVECs, integrity of VE-cadherin and cell proliferation. We expect that this system could be utilized further for understanding different mechanisms of apoptotic macrophage on the development of atherosclerosis.

Keywords

References

  1. Kim, M. H., Y. H. Jung, and D. H. Lee., 2015, "Cardiovascular diseases prevention and control campaign, 2015." Public Health Wkly Rep, Vol. 8(39), pp. 925-928.
  2. Sander, Dirk, et al., 2000, "Relationship between circadian blood pressure patterns and progression of early carotid atherosclerosis." Circulation, Vol. 102(13), pp. 1536-1541. https://doi.org/10.1161/01.CIR.102.13.1536
  3. Hansson GK, Hamsten A., 2016, Atherosclerosis, thrombosis, and vascular biology. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine. 25th ed. Philadelphia, PA: Elsevier Saunders: chap 70.
  4. Tabas, Ira., 2005, "Consequences and therapeutic implications of macrophage apoptosis in atherosclerosis." Arteriosclerosis, thrombosis, and vascular biology, Vol. 25(11), pp. 2255-2264. https://doi.org/10.1161/01.ATV.0000184783.04864.9f
  5. Silvestre-Roig, Carlos, et al., 2014, "Atherosclerotic Plaque Destabilization." Circulation research, Vol. 114(1), pp. 214-226. https://doi.org/10.1161/CIRCRESAHA.114.302355
  6. Kim, Seunggyu, et al., 2017, "Vasculature-on-a-chip for in vitro disease models." Bioengineering 4.1: 8. https://doi.org/10.3390/bioengineering4010008
  7. Jeon, Jessie S., et al., 2015, "Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation." Proceedings of the National Academy of Sciences, Vol. 112(1), pp. 214-219.
  8. Kim, YongTae, et al., 2014, "Probing nanoparticle translocation across the permeable endothelium in experimental atherosclerosis." Proceedings of the National Academy of Sciences, Vol. 111(3), pp. 1078-1083.
  9. Estrada, Rosendo, et al., 2011, "Microfluidic endothelial cell culture model to replicate disturbed flow conditions seen in atherosclerosis susceptible regions." Biomicrofluidics 5.3 : 032006.. https://doi.org/10.1063/1.3608137
  10. Vestweber, Dietmar., 2008, "VE-cadherin." Arteriosclerosis, thrombosis, and vascular biology, Vol. 28(2), pp. 223-232. https://doi.org/10.1161/ATVBAHA.107.158014
  11. Yakes, F. Michael, and Bennett Van Houten., 1997, "Mitochondrial DNA damage is more extensive and persists longer than nuclear DNA damage in human cells following oxidative stress." Proceedings of the National Academy of Sciences, Vol. 94(2), pp. 514-519.
  12. Chatzizisis, Yiannis S., et al., 2007, "Role of endothelial shear stress in the natural history of coronary atherosclerosis and vascular remodeling: molecular, cellular, and vascular behavior." Journal of the American College of Cardiology, Vol. 49(25), pp. 2379-2393. https://doi.org/10.1016/j.jacc.2007.02.059
  13. Miao, Hui, et al., 2005, "Effects of flow patterns on the localization and expression of VE-cadherin at vascular endothelial cell junctions: in vivo and in vitro investigations." Journal of vascular research, Vol. 42(1), pp. 77-89. https://doi.org/10.1159/000083094