대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
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항염증조절을 통한 자금정의 DSS 유도 궤양성 대장염 완화 효과

Mitigating Effect of Jageum-jung on Dextran Sulfate Sodium-induced Ulcerative Colitis through Anti-inflammatory Regulation

  • 최준용 (부산대학교한방병원 한방내과) ;
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 사은호 (다나은한방병원) ;
  • 사복석 (청신한약방) ;
  • 김기봉 (부산대학교한방병원 한방소아과)
  • Choi, Jun-yong (Dept. of Internal Medicine, Pusan National University Hospital) ;
  • Ahn, Sang-hyun (Dept. of anatomy, college of Korean Medicine, Se-Myung University) ;
  • Shih, Yin-hao (Danaeun Tradition Hospital) ;
  • Sa, Bok-suk (Chungsin Herbal Store) ;
  • Kim, Ki-bong (Dept. of Korean Pediatrics, Pusan National University Hospital)
  • 투고 : 2017.11.08
  • 심사 : 2017.12.27
  • 발행 : 2017.12.30
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초록

Objectives: This study investigated the anti-inflammatory effects of Jageum-jung extract on Dextran sulfate sodium (DSS-induced) ulcerative colitis in mice. Methods: Ulcerative colitis was induced by DSS in Balb/C male mice. Ten mice were assigned to each of four groups: Ctrl (control), UE (ulcerative colitis-induced), PT (treated with pentasaccharide after induction of ulcerative colitis), and JT (treated with Jageum-jung extract after induction of ulcerative colitis). The effects of Jageum-jung extract were measured by restoration of the length of the intestine, degree of mucosal damage as seen with histochemistry, and changes of p-IkB, iNOS, COX-2, and caspase-3 determined by immunohistochemistry. Results: The recovered intestinal length of the JT group was longer than that of the UE group. In the colon mucosa of JT group, hemorrhagic lesions were reduced, and the mucus barrier was recovered. This group also showed inhibited production of inflammatory enzymes (iNOS, COX-2) through regulation of proinflammatory enzyme (NF-kB, p65) activity in the colon. In addition, caspase 3 activation induced apoptosis. By GC/MS analysis, azetidine was identified. Conclusions: This study confirmed the anti-inflammatory effects of jageum-jung extract, and suggests the possibility of using Jageum-jung extract to treat ulcerative colitis. Further experiments and research on the mechanism of Jageum-jung effects are needed.

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참고문헌

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