Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-Proliferative Activity of Ethanol Extracts from Taxilli Ramulus (Taxillus chinensis (DC.) Danser) Through Cyclin D1 Proteasomal Degradation in Human Colorectal Cancer Cells

  • Park, Gwang Hun (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Song, Hun Min (Department of Medicinal Plant Resources, Andong National University) ;
  • Park, Su Bin (Department of Medicinal Plant Resources, Andong National University) ;
  • Park, Ji Hye (Department of Medicinal Plant Resources, Andong National University) ;
  • Shin, Myeong Su (Department of Medicinal Plant Resources, Andong National University) ;
  • Son, Ho-Jun (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Um, Yurry (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University)
  • Received : 2017.07.15
  • Accepted : 2017.10.16
  • Published : 2017.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we elucidated anti-cancer activity and potential molecular mechanism of 70% ethanol extracts from Taxilli Ramulus (Taxillus chinensis (DC.) Danser) (TR-E70) against human colorectal cancer cells. Anti-cell proliferative effect of TR-E70 was evaluated by MTT assay. The effect of TR-E70 on the expression of cyclin D1 in the protein and mRNA level was evaluated by Western blot and RT-PCR, respectively. TR-E70 suppressed the proliferation of human colorectal cancer cell lines, HCT116 and SW480. Although TR-E70 decreased cyclin D1 expression in protein and mRNA level, decreased level of cyclin D1 protein by TR-E70 more dramatically occurred than that of cyclin D1 mRNA. Cyclin D1 downregulation by TR-E70 was attenuated in presence of MG132. In addition, TR-E70 phosphorylated threonine-286 (T286) of cyclin D1. TR-E70-mediated cyclin D1 degradation was blocked in presence of LiCl as an inhibitor $GSK3{\beta}$ but not PD98059 as an ERK1/2 inhibitor and SB203580 as a p38 inhibitor. Our results suggest that TR-E70 may downregulate cyclin D1 as one of the potential anti-cancer targets through $GSK3{\beta}$-dependent cyclin D1 degradation. From these findings, TR-E70 has potential to be a candidate for the development of chemoprevention or therapeutic agents for human colorectal cancer.

Keywords

References

  1. Alao, J.P. 2007. The regulation of cyclin D1 degradation: roles in cancer development and the potential for therapeutic invention. Mol. Cancer 6:24.
  2. Alt, J.R., J.L. Cleveland, M. Hannink and J.A. Diehl. 2000. Phosphorylation-dependent regulation of cyclin D1 nuclear export and cyclin D1-dependent cellular transformation. Genes Dev. 14:3102-3114. https://doi.org/10.1101/gad.854900
  3. Bahnassy, A.A., A.R. Zekri, S. El-Houssini, A.M. El-Shehaby, M.R. Mahmoud, S. Abdallah and M. El-Serafi. 2004. Cyclin A and cyclin D1 as significant prognostic markers in colorectal cancer patients. BMC Gastroenterol. 23:22-24.
  4. Baker, D.D., M. Chu, U. Oza and V. Rajgarhia. 2007. The value of natural products to future pharmaceutical discovery. Nat. Prod. Rep. 24:1225-1244. https://doi.org/10.1039/b602241n
  5. Casanovas, O., F. Miro, J.M. Estanyol, E. Itarte, N. Agell and O. Bachs. 2000. Osmotic stress regulates the stability of cyclin D1 in a p38SAPK2-dependent manner. J. Biol. Chem. 275:35091-35097. https://doi.org/10.1074/jbc.M006324200
  6. Cragg, G.M., D.J. Newman and K.M. Snader. 1997. Natural products in drug discovery and development. J. Nat. Prod. 60:52-60. https://doi.org/10.1021/np9604893
  7. Diehl, J.A., M. Cheng, M.F. Roussel and C.J. Sherr. 1998. Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 12:3499-3511. https://doi.org/10.1101/gad.12.22.3499
  8. Eo, H.J., G.H. Park, H.M. Song, J.W. Lee, M.K. Kim, M.H. Lee, J.R. Lee, J.S. Koo and J.B. Jeong. 2015. Silymarin induces cyclin D1 proteasomal degradation via its phosphorylation of threonine-286 in human colorectal cancer cells. Int. Immunopharmacol. 24:1-6. https://doi.org/10.1016/j.intimp.2014.11.009
  9. Gillett, C., V. Fantl, R. Smith, C. Fisher, J. Bartek, C. Dickson, D. Barnes and G. Peters. 1994. Amplification and over-expression of cyclin D1 in breast cancer detected by immunohistochemical staining. Cancer Res. 54:1812-1817.
  10. Holland, T.A., J. Elder, J.M. McCloud, C. Hall, M. Deakin, A.A. Fryer, J.B. Elder and P.R. Hoban. 2001. Subcellular localisation of cyclin D1 protein in colorectal tumours is associated with p21(WAF1/CIP1) expression and correlates with patient survival. Int. J. Cancer 95:302-306. https://doi.org/10.1002/1097-0215(20010920)95:5<302::AID-IJC1052>3.0.CO;2-#
  11. Kato, A., A. Naiki-Ito, T. Nakazawa, K. Hayashi, I. Naitoh, K. Miyabe, S. Shimizu, H. Kondo, Y. Nishi, M. Yoshida, S. Umemura, Y. Hori, T. Mori, M. Tsutsumi, T. Kuno, S. Suzuki, H. Kato, H. Ohara, T. Joh and S. Takahashi. 2015. Chemopreventive effect of resveratrol and apocynin on pancreatic carcinogenesis via modulation of nuclear phosphorylated GSK3beta and ERK1/2. Oncotarget 6:42963-42975.
  12. Kim, M.K., G.H. Park, H.J. Eo, H.M. Song, J.W. Lee, M.J. Kwon, J.S. Koo and J.B. Jeong. 2015. Tanshinone I induces cyclin D1 proteasomal degradation in an ERK1/2 dependent way in human colorectal cancer cells. Fitoterapia 101: 162-168. https://doi.org/10.1016/j.fitote.2015.01.010
  13. Lee, S.H., M. Cekanova and S.J. Baek. 2008. Multiple mechanisms are involved in 6-gingerol-induced cell growth arrest and apoptosis in human colorectal cancer cells. Mol. Carcino-genesis 47:197-208. https://doi.org/10.1002/mc.20374
  14. Mermelshtein, A., A. Gerson, S. Walfisch, B. Delgado, G. Shechter-Maor, J. Delgado, A. Fich and L. Gheber. 2005. Expression of D-type cyclins in colon cancer and in cell lines from colon carcinomas. Brit. J. Cancer 93:338-345. https://doi.org/10.1038/sj.bjc.6602709
  15. Musgrove, E.A., C.E. Caldon, J. Barraclough, A. Stone and R.L. Sutherland. 2011. Cyclin D as a therapeutic target in cancer. Nat. Rev. Cancer 11:558-572. https://doi.org/10.1038/nrc3090
  16. Okabe, H., S.H. Lee, J. Phuchareon, D.G. Albertson, F. McCormick and O. Tetsu. 2006. A critical role for FBXW8 and MAPK in cyclin D1 degradation and cancer cell proliferation. PloS One 1:e128. https://doi.org/10.1371/journal.pone.0000128
  17. Park, G.H., H.M. Song and J.B. Jeong. 2016a. The coffee diterpene kahweol suppresses the cell proliferation by inducing cyclin D1 proteasomal degradation via ERK1/2, JNK and GKS3beta-dependent threonine-286 phosphorylation in human colorectal cancer cells. Food Chem. Toxicol. 95:142-148. https://doi.org/10.1016/j.fct.2016.07.008
  18. Park, G.H., J.H. Sung, H.M. Song and J.B. Jeong. 2016b. Anti-cancer activity of Psoralea fructus through the down-regulation of cyclin D1 and CDK4 in human colorectal cancer cells. BMC Complement. Altern. Med. 16:373. https://doi.org/10.1186/s12906-016-1364-x
  19. Park, G.H., S.C. Hong and J.B. Jeong. 2016c. Anticancer activity of the safflower seeds (Carthamus tinctorius L.) through inducing cyclin D1 proteasomal degradation in human colorectal cancer cells. Korean J. Plant Res. 29(3): 297-304. https://doi.org/10.7732/kjpr.2016.29.3.297
  20. Russell, A., M.A. Thompson, J. Hendley, L. Trute, J. Armes and D. Germain. 1999. Cyclin D1 and D3 associate with the SCF complex and are coordinately elevated in breast cancer. Oncogene 18:1983-1991. https://doi.org/10.1038/sj.onc.1202511
  21. Siegel, R.L., K.D. Miller, S.A. Fedewa, D.J. Ahnen, R.G.S. Meester, A. Barzi and A. Jemal. 2017. Colorectal cancer statistics, 2017. CA Cancer J. Clin. 67:177-193. https://doi.org/10.3322/caac.21395
  22. Thoms, H.C., M.G. Dunlop and L.A. Stark. 2007. p38-mediated inactivation of cyclin D1/cyclin-dependent kinase 4 stimulates nucleolar translocation of RelA and apoptosis in colorectal cancer cells. Cancer Res. 67:1660-1669. https://doi.org/10.1158/0008-5472.CAN-06-1038
  23. Wang, Y., M. Deng, S.Y. Zhang, Z.K. Zhou and W.X. Tian. 2008. Parasitic loranthus from Loranthaceae rather than Viscaceae potently inhibits fatty acid synthase and reduces body weight in mice. J. Ethnopharmacol. 118:473-478. https://doi.org/10.1016/j.jep.2008.05.016
  24. Wen, C.C., L.F. Shyur, J.T. Jan, P.H. Liang, C.J. Kuo, P. Arulselvan, J.B. Wu, S.C. Kuo and N.S. Yang. 2011. Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication. J. Trad. Complement. Med. 1:41-50. https://doi.org/10.1016/S2225-4110(16)30055-4
  25. Woo, I.S. and Y.H. Jung. 2017. Metronomic chemotherapy in metastatic colorectal cancer. Cancer Lett. 400:319-324. https://doi.org/10.1016/j.canlet.2017.02.034
  26. Zhang, L., S.R. Koyyalamudi, S.C. Jeong, N. Reddy, T. Bailey and T. Longvah. 2013. Immunomodulatory activities of polysaccharides isolated from Taxillus chinensis and Uncaria rhyncophylla. Carbohydr. Polym. 98:1458-1465. https://doi.org/10.1016/j.carbpol.2013.07.060
  27. Zhang, L., A.S. Ravipati, S.R. Koyyalamudi, S.C. Jeong, N. Reddy, P.T. Smith, J. Bartlett, K. Shanmugam, G. Munch and M.J. Wu. 2011. Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds. J. Agric. Food Chem. 59:12361-12367. https://doi.org/10.1021/jf203146e

Cited by

  1. 노랑붓꽃에서 분리된 Iridin의 독소루비신 유도 HK-2 세포 괴사에 대한 역할 및 암세포에 대한 작용 vol.31, pp.2, 2018, https://doi.org/10.7732/kjpr.2018.31.2.095