Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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L-Glycine Alleviates Furfural-Induced Growth Inhibition during Isobutanol Production in Escherichia coli

  • Song, Hun-Suk (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Jeon, Jong-Min (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Choi, Yong Keun (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Kim, Jun-Young (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Kim, Wooseong (Division of Infectious Diseases, Rhode Island Hospital, Alpert Medical School of Brown University) ;
  • Yoon, Jeong-Jun (Intelligent Sustainable Materials R&D Group, Korea Institute of Industrial Technology (KITECH)) ;
  • Park, Kyungmoon (Department of Biological and Chemical Engineering, Hongik University) ;
  • Ahn, Jungoh (Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB)) ;
  • Lee, Hongweon (Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB)) ;
  • Yang, Yung-Hun (Department of Biological Engineering, College of Engineering, Konkuk University)
  • Received : 2017.05.08
  • Accepted : 2017.09.26
  • Published : 2017.12.28
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Abstract

Lignocellulose is now a promising raw material for biofuel production. However, the lignin complex and crystalline cellulose require pretreatment steps for breakdown of the crystalline structure of cellulose for the generation of fermentable sugars. Moreover, several fermentation inhibitors are generated with sugar compounds, majorly furfural. The mitigation of these inhibitors is required for the further fermentation steps to proceed. Amino acids were investigated on furfural-induced growth inhibition in E. coli producing isobutanol. Glycine and serine were the most effective compounds against furfural. In minimal media, glycine conferred tolerance against furfural. From the $IC_{50}$ value for inhibitors in the production media, only glycine could alleviate growth arrest for furfural, where 6 mM glycine addition led to a slight increase in growth rate and isobutanol production from 2.6 to 2.8 g/l under furfural stress. Overexpression of glycine pathway genes did not lead to alleviation. However, addition of glycine to engineered strains blocked the growth arrest and increased the isobutanol production about 2.3-fold.

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