Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Lipolysis Effect of Daucosterol Isolated from Mulberry (Morus alba) Leaves

뽕잎으로부터 순수분리한 daucosterol의 lipolysis 효과

  • Li, Ke (Department of Horticultural Bioscience, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Lee, Mi Lim (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Que, Lu (Department of Horticultural Bioscience, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Li, Mae (Department of Horticultural Bioscience, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kang, Jum Soon (Department of Horticultural Bioscience, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Choi, Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University) ;
  • Kim, Kyung Mi (NOVAREX Co., Ltd. Life Science Institute) ;
  • Jung, Jae-Chul (NOVAREX Co., Ltd. Life Science Institute) ;
  • Hwang, Dae Youn (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Choi, Young Whan (Department of Horticultural Bioscience, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University)
  • 이커 (부산대학교 원예생명과학과/생명산업융합연구원) ;
  • 이미림 (부산대학교 바이오소재과학과) ;
  • 루쿼 (부산대학교 원예생명과학과/생명산업융합연구원) ;
  • 이매 (부산대학교 원예생명과학과/생명산업융합연구원) ;
  • 강점순 (부산대학교 원예생명과학과/생명산업융합연구원) ;
  • 최영현 (동의대학교 생화학과) ;
  • 김경미 ((주) 노바렉스) ;
  • 정재철 ((주) 노바렉스) ;
  • 황대연 (부산대학교 바이오소재과학과) ;
  • 최영환 (부산대학교 원예생명과학과/생명산업융합연구원)
  • Received : 2017.10.23
  • Accepted : 2017.12.13
  • Published : 2017.12.30
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Abstract

Plants are reservoirs of naturally occurring chemical compounds and of structurally diverse bioactive molecules. The aim of this investigation was to screen for the presence of phytochemicals responsible for the lipolysis activity in mulberry (Morus alba) leaves, which are important in traditional Asian medicinal plants. Powdered mulberry leaves were extracted with hexane, ethyl acetate, and methanol. Daucosterol was isolated from the EtOAc extract of mulberry leaves, and its structure was elucidated by NMR spectral analyses. The NMR assignments for the compound were determined using $^1H$, $^{13}C$, DEPT, COSY, HSQC, and HMBC NMR spectral data. Daucosterol showed a concentration-dependent lipolysis activity that may impart medicinal properties that can be exploited by medical practitioners for the treatment of various diseases. However, further studies should be conducted to elucidate additional mechanisms of daucosterol.

뽕나무는 약 40여 속과 1,000여 종이 있는 것으로 알려져 있으며, 항염증, 항진정, 지사작용, 노화억제 및 신경보호작용이 있는 것으로 알려져 있다. 본 연구에서는 아시아 지역에서 전통 한약재로 사용되는 뽕 나무 잎의 지방분해 활성에 관여하고 있는 물질을 스크린하기 위하여 뽕잎 분말을 헥산, 에틸 아세테이트 및 메탄올로 순차 추출하였다. 뽕 잎의 EtOAc 추출물로부터 daucosterol이 순수분리 되었으며, 그 구조는 $^1H$, $^{13}C$, DEPT, COSY, HSQC 및 HMBC 등의 NMR스펙트럼 분석에 의해 밝혀졌다. Daucosterol은 농도 의존적으로 지질분해 효과를 나타내었는데, 본 연구의 결과로부터 뽕나무 잎으로부터 순수분리한 daucosterol의 지분분해 활성은 다양한 질병을 치료하기 위한 천연물 소재 또는 지표성분으로서 활용이 가능할 것으로 생각된다. 그러나 보다 효율적으로 이용하기 위해서는 daucosterol의 비만에 관한 생리활성 기작에 대한 추가적인 연구가 필요할 것이다.

Keywords

References

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