Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Determination of the Origin of Angelica Roots using Angelica gigas Chloroplast Based SSR Markers

엽록체기반 SSR marker를 이용한 당귀의 기원 판별

  • Park, Sang Ik (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Hwangbo, Kyeong (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Gil, Jinsu (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Chung, Hee (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Kim, Ho Bang (Life Sciences Research Institute, Biomedic Co., Ltd.) ;
  • Kim, Ok Tae (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Seong Cheol (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Koo, Sung Cheol (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Um, Yurry (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Lee, Yi (Department of Industrial Plant Science and Technology, Chungbuk National University)
  • 박상익 (충북대학교 특용식물학과) ;
  • 황보경 (충북대학교 특용식물학과) ;
  • 길진수 (충북대학교 특용식물학과) ;
  • 정희 (충북대학교 특용식물학과) ;
  • 김호방 ((주)바이오메딕 생명과학연구소) ;
  • 김옥태 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김성철 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 구성철 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 엄유리 (국립산림과학원 산림약용자원연구소) ;
  • 이이 (충북대학교 특용식물학과)
  • Received : 2017.09.01
  • Accepted : 2017.10.31
  • Published : 2017.12.30
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Abstract

Background: In the herbal medicinal industry, Angelica gigas Nakai, Angelica sinensis (Oliv.) Diels. and Angelica acutiloba (Siebold & Zucc.) Kitag. are often confused, because the roots of the three species can not be distinguished by their appearance. This confusion can cause serious side effects. In this study, we determined the origins of Angelica roots distributed in the Korean market using the simple sequence repeat (SSR) markers developed based on the A. gigas chloroplast DNA sequence. Methods and Results: We collected twenty seven A. gigas and three A. acutiloba samples from the Seoul, Daegu, and Cheongju herbal medicinal markets. Fifty sections of one collection were mixed and ground to make a powder, which was used for DNA extraction using the cetyl trimethylammonium bromide (CTAB) method. Chloroplast based SSR markers were applied to the DNA for the determination of the species. In addition, polymorphism was found in eight samples. The phylogenetic analysis showed that the A. gigas roots collected from herbal medicinal markets were clearly discriminated from A. sinensis and A. acutiloba even though they were grouped into four clusters. Conclusions: This study showed that chloroplast based SSR markers would help the discrimination of Angelica roots in the Korean herbal medicinal industry and the markers are useful to prevent confusion between Angelica roots.

Keywords

References

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