Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
권호 표지
DOI QR코드

DOI QR Code

The Effect of Environmental Fine Bubble on the Production of Ginsenoside during the Growth Period of Ginseng Cultivation

인삼 재배시 생육기간 동안 환경적 요인인 미세기포수가 ginsenoside 생성에 미치는 영향

  • Ahn, Chul-Hyun (Korea National College of Agriculture and Fisheries)
  • Received : 2017.10.23
  • Accepted : 2017.12.20
  • Published : 2017.12.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate changes in ginsenoside by continuously treating fine bubble, which are mainly used for environmental purification, in 2-year-old ginseng. The ginsenoside content and composition of ginseng leaves and roots were analyzed for 4 months (120 days) after application of Fine bubble. As a result of treatment with common water in leaves, only Re of protopanaxatriol was significantly higher and As a result of treatment with fine buble, it was confirmed that protopanaxadiol Rb1, RC, Rb2 and Rd components were also increased. Especially, the increase of Re and Rb1 resulted in an increase of total ginsenoside. The ratio of PD / PT to ginseng was 0.811 in finebubble treated leaves and 1.28 in root. The fine bubble treatment induced the synthesis of ginsenoside from the roots and resulted in a PD / PT ratio of close to 1. Therefore, this study suggests a method of cultivating high quality ginseng using fine bubble water and suggests possibility of using it as a functional food material which can be used with leaves as well as roots.

본 연구는 환경정화에 주로 사용되는 미세기포를 2년 근 인삼 지속적으로 처리하여 인삼이 성장하면서 변화되는 형태와 ginsenoside 변화를 조사하는 융합적 연구이다. 인삼 재배시 미세기포수를 적용하여 4개월 동안(120일) 재배한 후 인삼의 잎과 뿌리의 부위별 ginsenoside 함량과 조성을 분석하였다. 잎에 일반수를 처리한 결과 protopanaxatriol(PPT) 계열 Re 함량만 월등히 높게 나타났지만 미세기포수를 처리한 결과 protopanaxadiol(PPD) 계열 Rb1, RC, Rb2, Rd 성분도 같이 증가시키는 것을 확인하였다. 특히 Re, Rb1이 다량 증가함으로써 전체적인 total ginsenoside가 증가하는 요인이 되었다. 인삼의 부위별 PD/PT 비율은 미세기포수를 처리한 잎에서는 0.811으로 나타나고 뿌리는 1.28로 나타났다. 이것은 미세기포수 처리가 뿌리에서 ginsenoside의 합성을 유도하여 PD/PT 비율이 1과 가까운 결가를 가져와 유용성분의 증가 및 고른 분포 이루어졌다고 판단된다. 따라서 미세버블수를 사용한 고품질 인삼을 생산하는 재배 방법을 제시하고 인삼의 뿌리와 더불어 잎도 기능성 식품 소재로 활용할 수 있는 가능성을 제시하였다.

Keywords

References

  1. J. Watanabe, K. W. Oh, H. S. Kim, M. Tanahashi, H. Kaneto, "A Non-opioid Mechanism in the Inhibitory Effect of Ginseng Saponins on Electrically Evoked Contractions of Guinea-Pig Ileum and Mouse vas Deferens" J. Pharmacobio-Dyn, Vol. 11, pp. 453-458, 1988. https://doi.org/10.1248/bpb1978.11.453
  2. I. I. Brekhman, "Panax ginseng" Gosudarst Isdatet Med, Vol. 2, pp. 10-24, 1957.
  3. T. Namba, "The Encyclopedia of Wakan -Yaku with Color Pictures" Hoikusha, Osaka, Japan, pp. 23-48, 1980.
  4. S. Shibata, O. Tanaka, K. Soma, Y. Iita, T. Ando, H. Nakamura, "Studies on saponins and sapogenins of ginseng: the structure of panaxatriol" Tetrahedron Lett, Vol. 3, pp. 207-213, 1965.
  5. S. Shibata, "Some chemical studies on ginseng" Proc. Intern. Ginseng Sym, pp. 69-76, 1974.
  6. J. D. Park, "Recent studies on the chemical constituents of Korean ginseng" Korean J. Ginseng Sci, Vol. 20, pp. 389-415, 1996.
  7. S. Sanata, N. Kondo, J. Shoji, O. Tanaka, S. Shibata, "Studies on the saponins of ginseng. I. Structure of ginseng-R0, Rb1, Rb2, Rc and Rd" Chem, Pharm. Bull, Vol. 22, pp. 421-428, 1974. https://doi.org/10.1248/cpb.22.421
  8. I. Kitagawa, T. Taniyama, H. Shibuya, T. Nota, M. Yoshikawa, "Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2); Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root" Yakugaku Zasshi, Vol. 107, pp. 495-505, 1987. https://doi.org/10.1248/yakushi1947.107.7_495
  9. T. Yokozawa, T. Kobayashi, H. Oura, Y. Kawashima, "Studies on the mechanism of the hypoglycemic activity of ginsenoside-Rb2 in streptozotocin-diabetic rats" Chem. Pharm. Bull, Vol. 33, pp. 869-872, 1985. https://doi.org/10.1248/cpb.33.869
  10. J. H. Park, "Sun ginseng-A new processed ginseng with fortified activity" Food Industry and Nutrition. Vol. 9, pp. 23-27, 2004.
  11. K. J. Choi, "The constituent of material ginseng and managment of Quality" Korea. J. Ginseng Sci. Vol. 15, pp. 247-256, 1991.
  12. Y. W. Kim, "Glucocorticoid receptor-induce down-regulation of metalloproteinse-9 (MMP-9) by ginseng component, panaxadiol (PD) and panaxatriol (PT), contributes to inhibition of the invasive capacity of HT1080 human fibrosacoma cell", proceeding of the 7 tH Int'l Symposium on ginseng, The korea Society of Ginseng, pp. 224-230, 1998.
  13. C. H. Ahn, "A convergent Study on Applying a fine bubble to ginseng seedling cultivation" Journal of the Korea Convergence Society Vol. 8, pp. 191-196, 2017. https://doi.org/10.15207/JKCS.2017.8.3.191
  14. J. S. Park, K. Kurata, "Application of microbubbles to hydroponics solution promotes lettuce growth" Horttechnology Vol. 19, No. 1, pp. 212-215, 2009.
  15. H. Ikeura, F. Kobayashi, M. Tamaki. "Ozone microbubble treatment at various water temperatures for the removalk of residual pesticides with negligible effects on the physical properties of lettuce and cherry tomatoes. J. Food sci. Vol. 78, pp 350-355, 2013. https://doi.org/10.1111/1750-3841.12007
  16. H. Ikeura, F. Kobayashi, M. Tamaki, "Removal of residual pesticide, fenitrothion, in vegetables by using ozone microbubbles generated by different methods", J Food Eng Vol. 103, pp. 345-349, 2011. https://doi.org/10.1016/j.jfoodeng.2010.11.002
  17. S. A. Lee, A. R. Youn, K. H. Kwon, B. S. Kim, "Washing effect of micro-bubbles and changes in quality of lettuce (Lactuca sativa L.) during storage", Korean J Food Preserv Vol. 16, pp. 321-326, 2009.
  18. S. J. Dong, R. Kiyama, "Characterization of oestrogenic activity of ginsenoside in MCF-7 cell using a customized DNA microarray" Food Chem. Vol. 113, pp. 672-678, 2009. https://doi.org/10.1016/j.foodchem.2008.07.100
  19. T. L. Chang, H. Y. Ding, Y. W. Kao, "Role of ginsenoside Rd in inhibiting 26S proteasome activity, J. Arg. Food Chem. Vol. 56, pp. 12011-12015, 2008. https://doi.org/10.1021/jf801427e
  20. R. D. Ye, N. L. Li, J. L. Han, X. W. Kong, R. Cao, Z. R. Rao, G. Zhao, "Neuroprotecttive effects og ginsenoside Rd against oxygen-glucose deprivation in Cultured Hippocampal neurons" Neurosci. Vol. 64, pp. 306-310, 2009.
  21. S. H. Jin, J. K. Park, K. Y. Nam, S. N. Park, N. P. Jung, "Korean red ginseng saponins with low ratio of protopanaxadiol and protopanaxatriol saponin improve scopolamine-induced learning disability and spatial work memory in mice" J. Ethnopharmacol. Vol. 66, pp. 123-129, 1999. https://doi.org/10.1016/S0378-8741(98)00190-1
  22. J. A. Rice M. T. Compardo, K. M. Scolari, L. L. Murphy, "Ginsenoside-Rc inhibits human breast cancer cell proliferaton in vitro. Mol. Bio. Cell. Vol. 11, pp. 452A-453A, 2000