Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Chemical Preservation Methods of Urine Sediment for Quality Control

정도관리를 위한 요침사의 화학적 보존방법

  • Cho, Soung Suck (Department of Clinical Laboratory Science, Shinsung University) ;
  • Kim, Myong Soo (Department of Laboratory Medicine, Samsung Medical Center) ;
  • Shin, Kyung-A (Department of Clinical Laboratory Science, Shinsung University)
  • 조성석 (신성대학교 임상병리과) ;
  • 김명수 (삼성서울병원 진단검사의학과) ;
  • 신경아 (신성대학교 임상병리과)
  • Received : 2017.09.20
  • Accepted : 2017.10.31
  • Published : 2017.12.31
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Abstract

There is greater standardization of quality control for microscopic examination of urine than for physicochemical test. In this study, we investigated whether it is possible to control the sediment accuracy by microscopic examination through the real thing by preserving the essential sediment with glutaraldehyde, which is required for the rationality of sediment quality control. A urine specimen was prepared using 2.5% glutaraldehyde as a preservation solution. Samples treated with urine preservatives confirmed the morphological deformation of the cells for four weeks at intervals of one week and confirmed whether they should be preserved for 4 weeks thereafter. After preparing the required sediment slide, two more slides were produced; one was stored in a refrigerator for, and the other was stored at room temperature. The morphological deformation of the specimen was confirmed. Glutaraldehyde has the effect of preserving the refrigerated essential sediments and storing them for up to 8 weeks, refrigerated storage after slide production, stabilized by 3 days. Moreover, after treatment with preservatives, the production of the slide and comparison between the measured values between the laboratories and examiners showed a low consistency. In conclusion, we showed that the urine sediment components can be preserved, and it can be used for quality control and education through real objects.

요침사 검사는 물리화학적 검사보다 정도관리의 표준화가 어려운 실정이다. 본 연구에서는 요침사 정도관리의 합리성을 위해 요침사 보존제인 글루타르알데히드로 요침사를 보존하여 실물을 통한 현미경 검사로 요침사 정도관리가 가능한지를 알아보고자 하였다. 2.5% 글루타르알데히드를 보존액으로 하여 표본을 제작하였다. 보존제를 처리한 검체는 1주일 간격으로 4주간 세포의 형태학적 변형을 확인하였고, 이후 4주간은 보존여부를 확인하였다. 요침사 슬라이드 제작 후 2개의 슬라이드를 제작하였으며, 하나는 냉장보관, 다른 하나는 실온 보관하여 검체의 형태학적 변형을 확인하였다. 글루타르알데히드는 요침사를 냉장 보관하여 8주까지 보존하는 효과가 있었으며, 슬라이드 제작 후에는 냉장 보관하여 3일까지 안정한 것으로 나타났다. 또한 보존제 처리 후 슬라이드 제작하여 검사실간, 검사자간판독을 비교한 결과 낮은 일치도를 보였다. 결론적으로 본 연구를 통해 요침사 성분을 보존할 수 있으며, 실물을 통한 정도관리 및 교육용으로 활용될 것으로 기대된다.

Keywords

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