Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Quali-Quantitative Analysis of Flavonoids for Mulberry Leaf and Fruit of 'Suhyang'

  • Ju, Wan-Taek (Department of Agricultural Biology, Sericulture & Apiculture Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kwon, O-Chul (Department of Agricultural Biology, Sericulture & Apiculture Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Lee, Min-Ki (Department of Agro-food Resources, Functional Food and Nutrition Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Hyun-Bok (Department of Agricultural Biology, Sericulture & Apiculture Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Sung, Gyoo-Byung (Department of Agricultural Biology, Sericulture & Apiculture Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Yong-Soon (Department of Agricultural Biology, Sericulture & Apiculture Division, National Institute of Agricultural Science, Rural Development Administration)
  • Received : 2017.11.06
  • Accepted : 2017.11.28
  • Published : 2017.12.31
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Abstract

BACKGROUND: Globally, mulberry (Morus sp.) is exploited for feeding leaf to silkworms in order to obtain silk fiber or for animal feedstock production. Also, mulberry fruit is known to a by-product that was produced from mulberry tree after harvesting leaves for silkworm rearing, as a yield and consumption of mulberry fruit was increased, it has been fixing to a newincome crop. Mulberry leaves and fruits are used for the health benefits of human beings. Mulberry contains various bioactive components, such as alkaloids and flavonoids. Mulberry flavonoids are an important part of the diet because of their effects on human nutrition. The flavonoids in mulberry leaf and fruit of 'Suhyang'(Morus alba L.) were determined. METHODS AND RESULTS: Flavonoids for mulberry leaf and fruit of 'Suhyang' were analysed using ultrahigh performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS)technique. An UPLC-DAD-QTOF/MS system was used, and identification of mulberry leaves constituents was carried out on the basis of the complementary information obtained from LC spectra, MS ions, and MS/MS fragments. The mulberry leaf (16 flavonoids) and fruit (9 flavonoids) were isolated and analyzed from Suhyang using UPLC-DAD-QTOF/MS chromatogram. To the best of our knowledge, Quercetin 3-O-(6"-O-malonyl) glucoside and quercetin 3-O-rutinoside (rutin) was detected on the highest content in leaf and fruit, respectively and further research will be devoted to evaluate their biological activity. CONCLUSION: Obtaining information about the concentration of functional materials in mulberry leaves could contribute to the development and promotion of processed, functional products and offer possible industrial use of 'Suhyang', holding promises to enhance the overall profitability of sericulture.

Keywords

References

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