International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지
DOI QR코드

DOI QR Code

Characteristics of genes in carotenoid cocoon color, Bombyx mori L.

  • Lee, Ju-Han (College of Agricultural and Life science, Kyungpook National University) ;
  • Kang, Min-Uk (Department of Analysis & Certification, FACT) ;
  • Park, Kwan-Ho (Department of Agricultural Biology, NAS, RDA) ;
  • Nho, Si-Kab (College of Agricultural and Life science, Kyungpook National University)
  • Received : 2017.08.03
  • Accepted : 2017.09.06
  • Published : 2017.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The cocoon's color of silkworm, Bombyx mori L. is usually white. But some are yellow, flesh and green colors because of modified characteristics. The yellow and flesh cocoons depend on carotenoid pigments, green cocoons are determined by flavonoid pigments. The cocoon's color is affected by the genes controlling penetration process from midgut to coelom and silk gland. Y (Yellow blood, 2-25.6) and I (Yellow-inhibitor, 9-16.2) genes are involved in the penetration process of carotenoid pigments from midgut to coelom, C (Outer-layer yellow cocoon, 12-7.2) and F (Flesh, 6-13.6) genes from coelom to silk gland. Therefore, the carotenoid cocoon's color depends on the genotype Y, I, C and F genes and their combination. Among them, C gene is sympathetic gene, which are known as C, CI and CD. C (Outer-layer yellow cocoon) genes make yellow cocoons on outer-layer and white cocoons on inter-layer, and CI (Inner-layer yellow cocoon) genes do yellow cocoons on inter-layer and dilute yellow cocoons on outer-layer. CD gene is known as making dilute yellow cocoons all layer. In this study, we have checked the dominance relation of C sympathetic genes among carotenoid genes for color cocoons by using strains related to the genes for color cocoons and investigated the aspect that pigments were penetrated in silk gland by action of each gene.

Keywords

References

  1. Chai CL, Zhang YJ, Sun WZ, Ding GS, Wang W, Liu YQ, et al. (2014) Analysis of a silkworm F1 hybrid with yellow cocoon generated by crossing two white-cocoon strains: Further evidences for the roles of Cameo2 and Carotenoid-binding protein in formation of yellow cocoon. Gene 534, 119-123. https://doi.org/10.1016/j.gene.2013.10.030
  2. Nho SK (1996) Characteristics of Korean native strains in the domesticated silkworm, Bombyx mori. Korean J Seric Sci 38(1), 57-72.
  3. Sakudoh T, Iizuka T, Narukawa J, Sezutsu H, Kobayashi I, Kuwazaki S, et al. (2010) A CD36-related Transmembrane Protein Is Coordinated with an Intracellular Lipid-binding Protein in Selective Carotenoid Transport for Cocoon Coloration. J Biol Chem 285, 7739-7751. https://doi.org/10.1074/jbc.M109.074435
  4. Sakudoh T, Sezutsu H, Nakashima T, Kobayash I, Fujimoto H, Uchino K, et al. (2007) Carotenoid silky coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene. Proc Natl Acad Sci USA 104, 8941-8946. https://doi.org/10.1073/pnas.0702860104
  5. Sakudoh T, Tsuchida K, Kataoka H (2005) BmStart1, a novel carotenoid-binding protein isoform from Bombyx mori, is orthologous to MLN64, a mammalian cholesterol transporter. Biochem Biophys Res Commun 336, 1125-1135. https://doi.org/10.1016/j.bbrc.2005.08.241
  6. Sakudoh T, Kuwazaki S, Iizuka T, Narukawa J, Yamamoto K, Uchino K, et al. (2013) CD36 homolog divergence is responsible for the selectivity of carotenoid species migration to the silk gland of the silkworm Bombyx mori. J Lipid Res 54(2), 482-495. https://doi.org/10.1194/jlr.M032771
  7. Tsuchida K, Arai M, Tanaka Y, Ishihara R, Ryan RO, Maekawa H (1998) Lipid transfer particle catalyzes transfer of carotenoids between lipophorins of Bombyx mori. Insect Biochem Mol Biol 28, 927-934. https://doi.org/10.1016/S0965-1748(98)00036-8
  8. Tsuchida K, Katagiri C, Tanaka Y, Tabunoki H, Sato R, Maekawa H, et al. (2004) The basis for colorless hemolymph and cocoons in the Y-gene recessive Bombyx mori mutants: a defect in the cellular uptake of carotenoids. J Insect Physiol 50, 975-983. https://doi.org/10.1016/j.jinsphys.2004.08.001
  9. Tsuchida K, Zeina E. Jouni, Gardetto J, Kobayashi Y, Tabunoki H, et al. (2004) Characterization of the carotenoid-binding protein of the Y-gene dominant mutants of Bombyx mori. J Insect Physiol 50, 363-372. https://doi.org/10.1016/j.jinsphys.2004.02.006
  10. Wang W, Huang MH, Dong XL, Chai CL, Pan CX, Tang H, et al. (2014) Combined Effect of Cameo2 and CBP on the Cellular Uptake of Lutein in the Silkworm, Bombyx mori. PLoS One 9, 86594. https://doi.org/10.1371/journal.pone.0086594

Cited by

  1. Crystallinity change of silkworm variety cocoons by heat treatment vol.42, pp.1, 2021, https://doi.org/10.7852/ijie.2021.42.1.7