한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제32권6호
  • /
  • Pages.531-535
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  • 2017
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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Leuconostoc mesenteroides를 이용한 브로콜리 발효물에 의한 Clostridium difficile의 생육 제어

Growth Inhibition of Clostridium difficile by Fermented Broccoli with Leuconostoc mesenteroides

  • 이영덕 (서원대학교 식품공학과) ;
  • 문기성 (한국교통대학교 생명공학과)
  • Lee, Young-Duck (Department of Food Science and Engineering, Seowon University) ;
  • Moon, Gi-Seong (Department of Biotechnology, Korea National University of Transportation)
  • 투고 : 2017.10.27
  • 심사 : 2017.11.15
  • 발행 : 2017.12.30
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초록

본 연구에서는 김치로부터 L. mesenteroides CJNU0041을 분리하여 16S rRNA 염기서열 분석을 통해, 동정하였으며, ${\beta}$-glucosidase 활성이 우수한 것으로 나타났다. L. mesenteroides CJNU0041를 이용한 브로콜리 발효 동안 생균수와 pH 및 ${\beta}$-glucosidase 활성에 대해 분석한 결과, 발효 시간은 48 hours이 적당한 것으로 판단되었다. 또한, 발효 후 생물 전환이 일어남을 HPLC 분석을 통해 확인하였다. 그리고, L. mesenteroides CJNU0041의 브로콜리 발효물에서 C. difficile에 대한 생육 억제 효과를 확인 할 수 있었다. 따라서, 본 연구를 통해 분리된 신규 L. mesenteroides CJNU0041을 C. difficile의 생육 제어를 위한 유산균 제재화가 가능할 것으로 판단되며, 브로콜리 발효물도 다양한 식품에 적용이 가능할 것으로 사료된다.

In this study, Leuconostoc mesenteroides CJNU0041 was isolated from Korean traditional food kimchi and antimicrobial activity of fermented broccoli with the isolate was tested against pathogenic Clostridium difficile. L. mesenteroides CJNU0041 showed higher glucosidase activity than other isolates. As the results of physiological properties such as pH and viable cell count during broccoli fermentation with L. mesenteroides CJNU0041, we confirmed that 48 hours was optimal fermentation time. As the results of metabolite analysis by HPLC, metabolites were changed during the fermentation. Especially, the growth of C. difficile was inhibited by the fermented broccoli. Therefore, L. mesenteroides CJNU0041 might be a candidate for improving the functionality of natural materials by lactic acid fermentation.

키워드

참고문헌

  1. Al-Jashaami, L.S., DuPont, H.L. Management of Clostridium difficile Infection. Gastroenterol. Hepatol. 12, 609-616 (2016).
  2. Kelly, C.P., Pothoulakis, C., Lamont, J.T. Clostridium difficile colitis. New Engl. J. Med., 330, 257-262 (1994). https://doi.org/10.1056/NEJM199401273300406
  3. McFarland L.V., Mulligan M.E., Kwok, R.Y., Stamm, W.E. Nocosomial acquisition of Clostridium difficile infection. New Engl. J. Med., 320, 204-210 (1989). https://doi.org/10.1056/NEJM198901263200402
  4. Bartlett, J.G. Clostridium difficile: history of its role as an enteric pathogen and the current state of knowledge about the organism. Clin. Infect. Dis. 18, 5265-5275 (1994).
  5. Cartmill, T.D., Panigrahi, H., Worsley, M.A., McCann, D.C., Nice, C.N., Keith, E. Management of diarrhoea due to Clostridium difficile. J. Hosp. Infect. 27, 1-15 (1994). https://doi.org/10.1016/0195-6701(94)90063-9
  6. Freeman, J., Wilcox, M.H. Antibiotics and Clostridium difficile. Microbes Infect. 1, 377-384 (1999). https://doi.org/10.1016/S1286-4579(99)80054-9
  7. Ackermann, G., Loffler, B., Tang-Feldman, Y.J., Cohen, S.H., Silva, J., Rodloff, A.C. Cloning and expression of Clostridium difficile toxin A gene(tcdA) by PCR amplication and use of an expression vector. Mol. Cell. Probe 18, 271- 274 (2004). https://doi.org/10.1016/j.mcp.2004.02.002
  8. Pothoulakis, C. Effect of Clostridium difficile toxins on epithelial cell barrier. Ann. NY Acad. Sci. 915, 347-356 (2000).
  9. Jung, S.M., Choi, S.I., Park, S.M. Heo, T.R. Antimicrobial Effect of Achyranthes japonica Nakai Extracts against Clostridium difficile. Korean J. Food Sci. Technol., 39, 564- 568 (2007).
  10. Leroy, F. De Vuyst, L. Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci. Technol., 15, 67-78 (2004). https://doi.org/10.1016/j.tifs.2003.09.004
  11. Vyas, U., Ranganathan, N. Probiotics, prebiotics, and synbiotics: Gut and beyond. Gastroenterol. Res. Pract., 2012, 1- 16 (2012).
  12. Jeppsson, B., Mangell, P., and Thorlacius, H.: Use of probiotics as prophylaxis for postoperative infections. Nutri., 3, 604-612 (2011). https://doi.org/10.3390/nu3050604
  13. Wallace, T.C., Guarner, F., Madsen, K., Cabana, M.D., Gibson, G., Hentges, E., and Sanders, M.E.: Human gut microbiota and its relationship to health and disease. Nutr. Rev., 69, 392-403 (2011). https://doi.org/10.1111/j.1753-4887.2011.00402.x
  14. Rodriguez, H., Curiel, J.A., Landete, J.M., de las Rivas, B., de Felipe, F. L., Gomez-Cordoves, C., Mancheno, J.M., Munoz, R.. Food phenolics and lactic acid bacteria. Int. J. Food Microbiol., 132, 79-90 (2009). https://doi.org/10.1016/j.ijfoodmicro.2009.03.025
  15. Chi, H., Kim, D.H., and Ji, G.E. Transformation of ginsenosides Rb2 and Rc from Panax ginseng by food microorganisms. Biol. Pharm. Bull., 28, 2102-2105 (2005). https://doi.org/10.1248/bpb.28.2102
  16. Kim, H.S., Kim, J.Y., Park, M.S., Zheng, H., Ji, G.E.. Cloning and expression of ${\beta}$-glucuronidase from Lactobacillus brevis in E. coli and application in bioconversion of baicalin and wogonoside. J. Microbiol. Biotechnol., 19, 1650-1655 (2009). https://doi.org/10.4014/jmb.0904.04053
  17. Rekha, C.R., Vijayalakshmi, G. Bioconversion of isoflavone glycosides to aglycones, mineral bioavailability and vitamin B complex in fermented soymilk by probiotic bacteria and yeast. J. Appl. Microbiol., 109, 1198-1208 (2010). https://doi.org/10.1111/j.1365-2672.2010.04745.x
  18. Holzapfel, W.H., Schillinger, U...The genus Leuconostoc. The prokaryotes (Balows, A., Truper, H.G., Dworkin, M., Harder, W., K.H.S chleifer, eds.). Springer-Verlag, Berlin, 1508-1534 (1992).
  19. Vijayendra, S.V.N., Palanivel, G., Mahadevamma, S., Tharanathan, R.N.. Physico-chemical characterization of an exopolysaccharide produced by a non-ropy strain of Leuconostoc sp. CFR 2181 isolated from dahi, an Indian traditional lactic acid fermented milk product. Carbohydr. Polym., 72, 300-307 (2008). https://doi.org/10.1016/j.carbpol.2007.08.016
  20. Kim, Y.H., Kim, H.J., Kin, J.Y., Choi, T.B., Kang, S.M. Strain Improvement of Leuconostoc mesenteroides as a Acid-Resistant Mutant and Effect on Kimchi fermentation as a Starter. Korean J. Microbiol. Biotechnol., 33, 41-5 (2005).
  21. Pepin, J. Clostridium difficile-associated diarrhea in a region of Quebec from 1991 to 2003: a changing pattern of disease severity. Cmaj., 171, 466-72 (2004). https://doi.org/10.1503/cmaj.1041104
  22. Dallal, R.M. Fulminant Clostridium difficile: an underappreciated and increasing cause of death and complications. Ann. Surg., 235, 363-72 (2002). https://doi.org/10.1097/00000658-200203000-00008
  23. Byun, T.J., et al., Clinical characteristics and changing epidemiology of Clostridium difficile-associated disease (CDAD). Korean J. Gastroenterol., 54, 13-9 (2009). https://doi.org/10.4166/kjg.2009.54.1.13
  24. Cheong, H.S. Therapeutic efficacy of metronidazole for patients with Clostridium difficile-associated diarrhea. Korean J Med., 72, 639-646 (2007).
  25. Kim, S.J. Molecular characterization of toxin A-negative, toxin B positive variant strains of Clostridium difficile isolated in Korea. Diagn. Microbiol. Infect. Dis., 67, 198-201 (2010). https://doi.org/10.1016/j.diagmicrobio.2010.01.007
  26. Naaber, P., Smidt, I., Jelena t epetova, Brilene. T., Annuk, H., Mikelsaar M. Inhibition of Clostridium difficile strains by intestinal Lactobacillus species. J. Med., Microbiol. 53, 551-554 (2004). https://doi.org/10.1099/jmm.0.45595-0
  27. Ratsep, M., Naaber, P., Koljalg, S., Smidt, I., Shkut, E., Sepp, E. Effect of Lactobacillus plantarum Strains on Clinical Isolates of Clostridium difficile in vitro. J. Prob. Health., 2014, 1-5 (2014).
  28. Rea, M.C., Alemayehu, D., R. Ross, P., Hill, C. Gut solutions to a gut problem: bacteriocins, probiotics and bacteriophage for control of Clostridium difficile infection. J. Med. Microbiol., 62, 1369-1378 (2013). https://doi.org/10.1099/jmm.0.058933-0
  29. Jung, S.M., Choi, S.I., Park, S.M., Heo, T.R. Synergistic Antimicrobial Effect of Achyranthes japonica Nakai Extracts and Bifidobacterium Supernatants Against Clostridium difficile. Korean J. Food Sci. Technol., 39, 564-568 (2007).
  30. Lee, J.S., Chung, M.J., Seo, J.G. In Vitro Evaluation of Antimicrobial Activity of Lactic Acid Bacteria against Clostridium difficile. Toxicol. Res., 29, 99-106 (2013). https://doi.org/10.5487/TR.2013.29.2.099