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녹조 원인 남세균 Microcystis aeruginosa의 생장을 억제하는 세균균주의 분리 및 남세균 생장 억제능 검정

Isolation of Bacterial Strains Inhibiting the Growth of Microcystis aeruginosa and Cyanobacterium Growth Inhibition Assay

  • 정선용 (전남대학교 공과대학 환경에너지공학과) ;
  • 고준일 (전남대학교 공과대학 환경에너지공학과) ;
  • 권범근 (조선이공대학교 생명환경화공과) ;
  • Chung, Seon-Yong (Department of Environment and Energy Engineering, College of Engineering, Chonnam National University) ;
  • Ko, Joon-IL (Department of Environment and Energy Engineering, College of Engineering, Chonnam National University) ;
  • Kwon, Bum-Gun (Department of Bioenvironmental & Chemical Engineering, Chosun College of Science and Technology) ;
  • Salma, Umme (Department of Environment and Energy Engineering, College of Engineering, Chonnam National University)
  • 투고 : 2017.10.13
  • 심사 : 2017.10.24
  • 발행 : 2017.11.30

초록

이 연구의 목적은 유해한 남세균인 Microcystis aeruginosa를 생물학적으로 제어하기 위해 남세균생장 억제미생물을 분리하고 그 효과를 조사하는 것이다. 이 연구에서 다양한 남세균생장 억제미생물이 분리되었고, 이중에서 M. aeruginosa의 생장을 효과적으로 억제하는 M1, M2, M3, M4로 명명된 4종의 균주를 분리하였다. 16S rRNA을 통해 동정된 M1~M4 균주는 모두 간균이고 그램 음성으로 나타났다. 분리된 단일종 뿐만 아니라, 혼합된 M1~M4 균주 4종의 공동배양이 M. aeruginosa를 효과적으로 처리하였다. 혼합 균주의 접종 2일 후에 약 50%의 클로로필 a가 감소되었고, 4일 후에 약 70%, 7일 후에 약 80%의 클로로필 a가 감소되었다. 이들 결과는 M1~M4 남세균생장 억제 균주가 유해한 M. aeruginosa를 제어하는데 기여할 것으로 생각된다.

The objective of this study was to isolate algae growth inhibiting microorganism to biologically control Microcystis aeruginosa, which is a harmful cyanobacterium. Various bacterial strains were isolated in this study, and four bacterial strains of M1~M4 exhibited remarkable growth inhibiting activity against M. aeruginosa. Based on the 16S rRNA analysis, the isolated M1~M4 strains were identified, and isolated four strains were rod-type and gram-negative. In particular, as well as respective single strain, co-culture of the isolated M1~M4 strains showed obvious algicidal activity against M. aeruginosa. When mixed four strains were inoculated, about 50% of the chlorophyll a was reduced after two days, about 70% after four days, and about 80% after seven days. From these results mentioned above, the four bacterial strains may contribute to the control of harmful M. aeruginosa.

키워드

참고문헌

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