International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Induction of Prostaglandin E2 by Porphyromonas gingivalis in Human Dental Pulp Cells

  • Kim, So-Hee (Department of Oral Microbiology, School of Dentistry, Chonnam National University) ;
  • Paek, Yun-Woong (Department of Physical Therapy, Gwangju Health University) ;
  • Kang, In-Chol (Department of Oral Microbiology, School of Dentistry, Chonnam National University)
  • Received : 2017.11.20
  • Accepted : 2017.12.13
  • Published : 2017.12.31
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Abstract

Cyclooxygenase-2 (COX-2)-mediated prostaglandin $E_2$ ($PGE_2$) plays a key role in development and progression of inflammatory responses and Porphyromonas gingivalis is a common endodontic pathogen. In this study, we investigated induction of COX-2 and $PGE_2$ by P. gingivalis in human dental pulp cells (HDPCs). P. gingivalis increased expression of COX-2, but not that of COX-1. Increased levels of $PGE_2$ were released from P. gingivalis-infected HDPCs and this $PGE_2$ increase was blocked by celecoxib, a selective COX-2 inhibitor. P. gingivalis activated all three types of mitogen-activated protein kinases (MAPKs). P. gingivalis-induced activation of nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) was demonstrated by the results of phosphorylation of $NF-{\kappa}B$ p65 and degradation of inhibitor of ${\kappa}B-{\alpha}$ ($I{\kappa}B-{\alpha}$). Pharmacological inhibition of each of the three types of MAPKs and $NF-{\kappa}B$ substantially attenuated P. gingivalis-induced $PGE_2$ production. These results suggest that P. gingivalis should promote endodontic inflammation by stimulating dental pulp cells to produce $PGE_2$.

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References

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