대한한의학방제학회지 (Herbal Formula Science)

  • 제25권4호
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  • Pages.483-497
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  • 2017
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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스트레스 유발 마우스모델에서 뇌염증 및 신경행동 장애 변화

Neuro-inflammation induced by restraint stress causes impairs neurobehavior in mice

  • 오태우 (한국한의학연구원 한의기술응용센터) ;
  • 도현주 (한국한의학연구원 한의기술응용센터) ;
  • 김광연 (한국한의학연구원 한의기술응용센터) ;
  • 김영우 (대구한의대학교 한의과대학) ;
  • 이병욱 (동국대학교 한의과대학) ;
  • 마진열 (한국한의학연구원 한의기술응용센터) ;
  • 박광일 (한국한의학연구원 한의기술응용센터)
  • Oh, Tae woo (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Do, Hyun Ju (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Kwang-Youn (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Kim, Young Woo (College of Oriental Medicine Deagu Haany University) ;
  • Lee, Byung Wook (College of Oriental Medicine Dongguk University) ;
  • Ma, Jin Yeul (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Park, Kwang Il (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
  • 투고 : 2017.11.08
  • 심사 : 2017.11.21
  • 발행 : 2017.11.30
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초록

Background : Behavioral stress has been suggested as one of the significant factors that is able to disrupt physiological systems and cause depression as well as changes in various body systems. The stressful events can alter cognition, learning, memory and emotional responses, resulting in mental disorders such as depression and anxiety. Results : We used a restraint stress model to evaluate the alteration of behavior and stress-related blood parameter. The animals were randomly divided into two groups of five animals each group. Furthermore, we assessed the change of body weight to evaluate the locomotor activity as well as status of emotional and anxiety in mice. After 7 days of restraint stress, the body weight had significantly decreased in the restraint stress group compared with the control group. We also observed stress-associated behavioral alterations, as there was a significant decrease in open field and forced swim test, whereas the immobilization time was significantly increased in the stress group compared to the control group. We observed the morphological changes of neuronal death and microglia by immunohistochemistry and western blot. In our study restraint stress did not cause change in neuronal cell density in the frontal cortex and CA1 hippocampus region, but there was a trend for an increased COX-2 and iNOS protein expression and microglia (CD11b) in brain, which is restraint stress. Conclusion : Our study, there were significant alterations observed in the behavioral studies. We found that mice undergoing restraint stress changed behavior, confirming the increased expression of inflammatory factors in the brain.

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