한국응용곤충학회지 (Korean journal of applied entomology)

  • 제56권4호
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  • Pages.331-338
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  • 2017
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
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국내에서 개발된 제초제저항성 벼(밀양 204호, 익산 483호)의 비표적 곤충과 거미에 미치는 생리적 영향

Physiological Effects of Herbicide-resistant Genetically Modified Rice (Milyang 204 and Iksan 483) Developed in Korea on Non-target Insects and a Spider

  • 김영호 (경북대학교 생태환경관광학부 생물응용전공)
  • Kim, Young Ho (Department of Applied Biology, Kyungpook National University)
  • 투고 : 2017.08.07
  • 심사 : 2017.10.23
  • 발행 : 2017.12.01
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초록

본 연구에서 국내에서 개발된 제초제저항성 GM 벼인 밀양 204호와 익산 483호가 비표적 곤충과 거미에 미치는 영향을 평가하였다. GM 벼와 non-GM 벼에 대한 보리수염진딧물과 벼멸구의 기주선호도에는 차이가 없었으며, GM 벼를 섭식한 벼멸구를 포식한 황산적거미의 체중 변화에도 통계적 유의성이 없었다. 밀양 204호와 그 대조 모본 non-GM 벼에서 서식한 벼멸구를 포식한 황산적거미의 생존율에는 차이가 없었으나, 벼멸구를 통해 익산 483호에 노출된 황산적거미의 생존율은 대조 모본 non-GM 벼에 비해 통계적으로 낮은 생존율을 나타내었다. 또한 익산 483호의 화분을 섭식한 꿀벌 유충은 밀양 204호와 다른 non-GM 벼 화분을 제공하였을 때 보다 현저하게 긴 용기간을 보였다. 농업생태계에서 중요한 포식 천적인 황산적거미와 양봉 산업으로서 중요한 기능을 하는 꿀벌에 GM 벼가 부정적인 영향을 보임에 따라 국내에서 개발된 제초제저항성 GM 벼의 농업 환경 노출 이전에 충분한 추가 연구와 안전성 평가가 선행되어야 할 것으로 사료된다.

In the present study, we investigated the effects of two herbicide-resistant genetically modified rice (GM rice) varieties, Milyang 204 and Iksan 483, recently developed in Korea on non-target insects and a spider. No difference in host preferences of the English grain aphid Sitobion avenae and the brown planthopper Nilaparvata lugens were observed between GM rice and non-GM rice. Wolf spider Pirata subpiraticus, feeding on N. lugens reared on GM rice or non-GM rice, revealed no significant difference in body weight. P. subpiraticus, fed with N. lugens reared on Milyang 204, showed survival rates similar to that in P. subpiraticus fed with N. lugens reared on non-GM rice. However, P. subpiraticus feeding on N. lugens reared on Iksan 483 demonstrated significantly lower survival rates than that in P. subpiraticus feeding on N. lugens reared on Milyang 204 or non-GM rice. In addition, when larvae of the western honeybee Apis mellifera were supplied with Iksan 483 pollen, a significantly longer pupal period occurred, as compared with that of A. mellifera supplied with pollen of Milyang 204 or non-GM rice. As GM rice has negative effects on P. subpiraticus, which is an important predator in agricultural ecosystems, and on A. mellifera, which plays important roles in pollination and honey production, additional studies on risk assessment of GM rice should be conducted before releasing newly developed herbicide-resistant GM rice to the agricultural environment.

키워드

참고문헌

  1. Burgio, G., Dinelli, G., Marotti, I., Zurla, M., Bosi, S., Lanzoni, A., 2011. Bt-toxin uptake by the non-target herbivore, Myzus persicae (Hemiptera: Aphididae), feeding on transgenic oilseed rape in laboratory conditions. Bull. Entomol. Res. 101, 241-247. https://doi.org/10.1017/S0007485310000441
  2. Burgio, G., Lanzoni, A., Accinelli, G., Dinelli, G., Bonetti, A., Marotti, I., Ramilli, F., 2007. Evaluation of Bt-toxin uptake by the non-target herbivore, Myzus persicae (Hemiptera: Aphididae), feeding on transgenic oilseed rape. Bull. Entomol. Res. 97, 211-215. https://doi.org/10.1017/S0007485307004920
  3. Haughton, A.J., Champion, G.T., Hawes, C., Heard, M.S., Brooks, D.R., Bohan, D.A., Clark, S.J., Dewar, A.M., Firbank, L.G., Osborne, J.L., Perry, J.N., Rothery, P., Roy, D.B., Scott, R.J., Woiwod, I.P., Birchall, C., Skellern, M.P., Walker, J.H., Baker, P., Browne, E.L., Dewar, A.J., Garner, B.H., Haylock, L.A., Horne, S.L., Mason, N.S., Sands, R.J., Walker, M.J., 2003. Invertebrate responses to the management of genetically modified herbicide-tolerant and conventional spring crops. II. Within-field epigeal and aerial arthropods. Philos. Trans. R. Soc. Lond. B Biol. Sci. 358, 1863-1877. https://doi.org/10.1098/rstb.2003.1408
  4. James, C., 2016. Global status of commercialized biotech/GM crops: 2016., ISAAA Briefs No. 52, Ithaka, NY.
  5. Kim, M.C., Ahn, J.H., Shin, H.C., Kim, T., Ryu, T.H., Kim, D.H., Song, H.G., Lee, G.H., Ka, J.O., 2008a. Molecular analysis of bacterial community structures in paddy soils for environmental risk assessment with two varieties of genetically modified rice, Iksan 483 and Milyang 204. J. Microbiol. Biotechnol. 18, 207-218.
  6. Kim, Y.H., Hwang, C.E., Kim, T.S., Lee, J.H., Lee, S.H., 2012a. Assessment of potential impacts due to unintentionally released Bt maize plants on non-target aphid Rhopalosiphum padi (Hemiptera: Aphididae). J. Asia-Pac. Entomol. 15, 443-446. https://doi.org/10.1016/j.aspen.2012.01.005
  7. Kim, Y.H., Hwang, C.E., Kim, T.S., Lee, S.H., 2012b. Risk assessment system establishment for evaluating the potential impacts of imported Bacillus thuringiensis maize on a non-target insect, Tenebrio molitor. J. Asia-Pac. Entomol. 15, 225-229. https://doi.org/10.1016/j.aspen.2011.11.003
  8. Kim, Y.H., Kang, J.S., Kim, J.I., Kwon, M., Lee, S., Cho, H.S., Lee, S.H., 2008b. Effects of Bt transgenic Chinese cabbage on the herbivore Mamestra brassicae (Lepidoptera : Noctuidae) and its parasitoid Microplitis mediator (Hymenoptera : Braconidae). J. Econ. Entomol. 101, 1134-1139. https://doi.org/10.1093/jee/101.4.1134
  9. Kim, Y.H., Kim, H., Lee, S., Lee, S.H., 2008c. Effects of Bt transgenic Chinese cabbage pollen expressing Bacillus thuringiensis Cry1Ac toxin on the non-target insect Bombyx mori (Lepidoptera: Bombyxidae) larvae. J. Asia-Pac. Entomol. 11, 107-110. https://doi.org/10.1016/j.aspen.2008.05.004
  10. KRIBB, 2015. White paper for biosafety 2015, Korea Research Institute of Bioscience and Biotechnology.
  11. KRIBB, 2017. Major statistics data for genetically modified organism in 2016, Korea Research Institute of Bioscience and Biotechnology.
  12. Lee, B., Kim, C.G., Park, J.Y., Park, K.W., Kim, H.J., Yi, H., Jeong, S.C., Yoon, W.K., Kim, H.M., 2009. Monitoring the occurrence of genetically modified soybean and maize in cultivated fields and along the transportation routes of the Incheon Port in South Korea. Food Control 20, 250-254. https://doi.org/10.1016/j.foodcont.2008.05.006
  13. Li, K., Tian, J., Wang, Q., Chen, Q., Chen, M., Wang, H., Zhou, Y., Peng, Y., Xiao, J., Ye, G., 2011. Application of a novel method PCR-ligase detection reaction for tracking predator-prey trophic links in insect-resistant GM rice ecosystem. Ecotoxicology 20, 2090-2100. https://doi.org/10.1007/s10646-011-0789-8
  14. Nam, K.J., Kim, Y.-J., Moon, D.-B., Nam, K.-H., Pack, I.S., Park, J.-H., Jeong, S.-C., Harn, C.H., Kim, C.-G. 2014. Effects of Bt cabbage (Brassica oleracea) on the host preference and performance of the green peach aphid, Myzus persicae Sulzer (Hemiptera: Aphididae). Korean J. Appl. Entomol. 53, 193-197. https://doi.org/10.5656/KSAE.2014.02.1.084
  15. Oh, S.-D., Shin, H., Sohn, S.-I., Lee, K., Kim, H.-J., Ryu, T.-H., Lee, J.-Y., Park, B.S., Kweon, S.J., Suh, S.-C., Park, J.-S., 2011. Evaluation and assessment of biosafety for Bacillus thuringiensis (Bt)-transgenic rice: Responses of Daphnia magna fed on Bt-transgenic rice variety. Appl. Biol. Chem. 54, 296-302. https://doi.org/10.3839/jabc.2011.048
  16. Park, K.W., Lee, B., Kim, C.G., Kim, D.Y., Park, J.Y., Ko, E.M., Jeong, S.C., Choi, K.H., Yoon, W.K., Kim, H.M., 2010. Monitoring the occurrence of genetically modified maize at a grain receiving port and along transportation routes in the Republic of Korea. Food Control 21, 456-461. https://doi.org/10.1016/j.foodcont.2009.07.006
  17. Raps, A., Kehr, J., Gugerli, P., Moar, W.J., Bigler, F., Hilbeck, A., 2001. Immunological analysis of phloem sap of Bacillus thuringiensis corn and of the nontarget herbivore Rhopalosiphum padi (Homoptera: Aphididae) for the presence of Cry1Ab. Mol. Ecol. 10, 525-533. https://doi.org/10.1046/j.1365-294x.2001.01236.x
  18. RDA, 2007. Studies on the environmental safety assessment of herbicide tolerant genetically modified rice, Iksan 483 & Milyang 204, Rural Development Administration.
  19. Romeis, J., Meissle, M., 2011. Non-target risk assessment of Bt crops - Cry protein uptake by aphids. J. Appl. Entomol. 135, 1-6. https://doi.org/10.1111/j.1439-0418.2010.01546.x
  20. Ryu, T.-H., Kim, D.-H., 2006. Current status of risk assessment and development of genetically modified rice. Biosafety 7, 20-32.
  21. Schutte, G., Eckerstorfer, M., Rastelli, V., Reichenbecher, W., Restrepo-Vassalli, S., Ruohonen-Lehto, M., Saucy, A.W., Mertens, M., 2017. Herbicide resistance and biodiversity: agronomic and environmental aspects of genetically modified herbicide-resistant plants. Environ. Sci. Eur. 29, 5. https://doi.org/10.1186/s12302-016-0100-y