Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지
DOI QR코드

DOI QR Code

An Experimental Study on Single-dose and 4 Weeks Repeated-dose Toxicity of Aconitum ciliare Decaisne Pharmacopuncture

초오약침의 단회 및 4주 반복 투여 독성에 관한 실험적 연구

  • Lee, Hyun Su (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Yun Kyu (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Bong Hyo (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jae Soo (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lim, Sung Chul (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Hyun-Jong (Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University)
  • 이현수 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이윤규 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이봉효 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 김재수 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이현종 (대구한의대학교 한의과대학 침구경혈학교실)
  • Received : 2017.11.29
  • Accepted : 2017.12.15
  • Published : 2017.12.27
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was performed to examine the toxicity of Aconitum ciliare Decaisne pharmacopuncture(ADP). Methods : The toxicity was evaluated for lethal dose for 50 percent kill(LD50), single dose and repeated dose for 4 weeks. Toxic symptoms, weight measurement, hematological test, blood biochemical test, visual examination and weight measurement of major organs and histopathological test were observed for 4 weeks. Dose of 300, 600, 1,200, 2,400, 3,600, 4,800, 6,000, 7,200 mg/kg in the LD50 experiment, 300, 600, 1,200 mg/kg/day in the single experiment, 150, 300, 600 mg/kg/day in 4 weeks experiments were injected into BALB/c mice. The ADP was injected into ST36 of the right leg. Normal saline solution of same volume was used for control group. In 24 hours after the last treatment, blood samples were taken after anesthesia by inhalation of ethyl ether. After that, the BALB/c mice were euthanized. Their heart, lungs, kidneys, liver and reproductive organs were removed and weighed. Histopathological evaluation was also performed. Results : ADP's LD50 was measured at 6,000 mg/kg. In both single and repeated dose toxicity test, no BALB/c mouse died during the experiments. ADP treatment for 4 weeks did not show any significant changes in toxic symptoms, weight measurement, hematological test, blood biochemical test, visual examination and weight measurement of major organs and histopathological test. Conclusions : As a result, ADP's LD50 was 6,000 mg/kg and repeated dose at a concentration of 600 mg/kg or less is considered to be not harmful for clinical treatment.

Keywords

References

  1. Yu CG, Gwon GR. The Bibilographic studies on Aconiti ciliare Tuber and Radix Aconiti. Journal of pharmacopuncture institute. 2001 ; 4(2) : 87-93.
  2. Oriental medical schools Herbology Compilation Committee. Herbology. Seoul. Yeongrimsa. 2007 : 311-2.
  3. Lee SJ. Bonchogangmok. Beijing. People's health publisher. 1977 : 1174-83.
  4. Kim CO, Kim HD. State examination Herbology. Seoul. Gunja publisher. 2007 : 114.
  5. Kim CM, Shin MK, Lee KS, Ahn DK. The encyclopedia of oriental herbal medicine. Seoul. Jungdam publisher. 2004 : 4280-6.
  6. Jang WS. Clinical Herbology. Gyeongsan. Daegu Haany University publishing department. 2015 : 290-1.
  7. Lee JM, Jang WS, Seo BI. A philological study on poisoning of Aconiti radix. The Journal of Applied Oriental Medicine. 2009 ; 9(1) : 23-41.
  8. Lim TH, Park KY. A case of aconitine poisoning with transient tetraplegia and hypocalcemia. Kor J of Med. 1998 ; 55(6) : 1093-6.
  9. Choi DI, Jin YH, Lee JB, Aconitine Intoxication Following Ingestion of Folk Recipes Containing Aconitum Species. J of The Kor Society of Emergency Med. 2002 ; 13(2) : 175-180.
  10. Kim MR. Ventricular Arrhythmia Following Aconitine Ingestion- 2 Case Reports-. J of The Korean Society of Clinical Toxicology. 2006 ; 4(2) : 180-6.
  11. Lee SI. Herbology. Seoul. Daeseong publisher. 1981 : 551.
  12. Yang CY, Jeong B, Go RM, Lee WC, Ban JG, Jeong GM. Poisonous traditional Chinese medicine counsel of play. Beijing. zhongguoyiyao science and technology publishing house. 1993 : 116-20.
  13. Kim YK. Studies on the Liver Toxicities with Different Dosage of Wild Aconiti Tuber Decoction. Seoul : Kyung Hee University. 2003.
  14. Kim HK, Lee HW, Jeon WK. Effects of Various Processing methods on the Contents of Alaloids in the Cho O(Aconiti Cilare Tuber). Kor J. Pharmacogn. 2002 ; 33(4) : 296-300.
  15. Korea Institute of Origntal Medicine. A Study of Various Processing methods on the contents of Aconiti Cliare Tuber. Korea Institute of Origntal Medicine Report. 2001 : 1-30.
  16. Korea Institute of Origntal Medicine. A Study on the cytotoxicity under before and after Various Processing methods of Aconiti Cliare Tuber. Korea Institute of Origntal Medicine Report. 2001 : 31-9.
  17. Kim JH. Kang SB. Effect of Radix Aconiti manufactured by Gamdutang on hepatic and renal fuction of rats. The Journal of Korean Medicine. 1994 ; 15(1) : 117-29.
  18. Kang SI. Experimental studies on the Safety of Aconiti Tuber Extract Solution for herb-acupuncture. Seoul : Kyung Hee University. 1996
  19. Connell DW, Yu QJ, Verma V. Influence of exposure time on toxicity-An overview. Toxicology. 2016 ; 29 : 355-6.
  20. Barlow SM, Greig JB, Bridges JW, Carere A, Carpy AJ, Galli CL et al. Harard identification by methods of animal-based toxicology. Food Chem Toxicology. 2002 ; 40 : 145-91. https://doi.org/10.1016/S0278-6915(01)00117-X
  21. Choi SY. Toxicology. Ulsan. Ulsan University publishing department. 1987 : 189-207, 298-9.
  22. Young Na Yum, Jae Ho Oh, Sheen Hee Kim, Myung Sil Hwang, Gi Dae Kim, Ji On Kim, Ki Taek Nam, Tae Seok Kang, Ki-Hwa Yang, and Dae Hyun Cho. Toxicity Study of 3-Monochloropropane- 1,2-diol using Proteomics Technique. The annual report of national toxicology program in Korea. 2003 ; 2(1) : 1-8.
  23. Jung SY, Kim GY, Kim BH, Jo YB. Toxicology. Seoul. Donghwa technology. 2000 : 15-21 , 118-94.
  24. Ko YS, Yun SH, Kim MK, Song YS, Shin BC. Literatural Study on Toxicity of Herbs used in . Korean J. Oriental Physiology & Pathology. 2005 ; 19(5) : 1120-8.
  25. Kim SY, Mun AR. Kidney toxicity assessment for the biomarkers discovery research. J of Pharmacology. 2010 ; 21 : 33-42.

Cited by

  1. 초오 약침액의 13주 반복 시술 독성에 관한 연구 vol.35, pp.3, 2018, https://doi.org/10.14406/acu.2018.022
  2. 원엽모고채 약침의 설치류에 대한 단회 및 반복 독성연구 vol.36, pp.2, 2019, https://doi.org/10.14406/acu.2019.013
  3. Repeated-Dose Toxicity Testing of Scolopendrid Pharmacopuncture in Sprague-Dawley Rats vol.37, pp.2, 2020, https://doi.org/10.13045/jar.2020.00059