The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Phylogenetic Analysis of Downy Mildew Caused by Peronospora destructor and a Method of Detection by PCR

양파 노균병균 Peronospora destructor의 분자계통학적 유연관계 분석과 PCR 검출기술 개발

  • Back, Chang-Gi (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science) ;
  • Hwang, Sun-Kyung (Technology Services Division, National Institute of Horticultural and Herbal Science) ;
  • Park, Mi jeong (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science) ;
  • Kwon, Young-Seok (Vegetable Research Division, National Institute of Horticultural and Herbal Science) ;
  • Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University) ;
  • Park, Jong-Han (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science)
  • 백창기 (국립원예특작과학원 원예특작환경과) ;
  • 황선경 (국립원예특작과학원 기술지원과) ;
  • 박미정 (국립원예특작과학원 원예특작환경과) ;
  • 권영석 (국립원예특작과학원 채소과) ;
  • 정희영 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 박종한 (국립원예특작과학원 원예특작환경과)
  • Received : 2017.11.07
  • Accepted : 2017.11.25
  • Published : 2017.12.01
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Abstract

Onion downy mildew, caused by Peronospora destructor, is a major disease in onion cultivation areas in Korea. The causal fungi were collected and analyzed based on sequence similarity and molecular phylogenetic relationships of multi-gene sequences, including the internal transcribed spacer (ITS) region. All isolates from Changnyeong-gun, Hamyang-gun, and Hapcheon-gun in Gyeongnam province, and Muan-gun, Haenam-gun, and Sinan-gun in Jeonnam province were identical in the four types of gene sequences, indicating they were genetically the same strains. In this study, a PCR method was developed based on the ITS gene sequences to amplify the specific DNA fragment for P. destructor only. The detection limit of was total genomic DNA of the P. destructor and the plant $0.7ng/{\mu}L$. Therefore, the developed PCR method could be used to detect P. destructor effectively from symptomless onion leaves.

우리나라 양파 재배지의 주요 곰팡이병인 양파 노균병의 원인균 Peronospora destructor를 채집하여, internal transcribed spacer (ITS) 영역을 포함한 4개 유전자의 염기서열을 분석하여 상동성 비교와 분자계통학적 유연관계를 분석하였다. 그 결과, 경남 창녕군, 함안군, 합천군과 전남 무안군, 신안군, 해남군에 발생하는 P. destructor의 4종의 유전자 염기서열 모두 100% 상동성을 보였고, 유연관계 분석에서도 모두 동일한 계통으로 확인되었다. 본 연구에서는 ITS 영역 유전자 염기서열을 이용하여 P. destructor 검출용 PCR법을 개발하였고, 노균병균만을 특이적으로 검출하였다. 또한 식물체를 포함한 total genomic DNA로 검출한계를 검정한 결과, $0.7ng/{\mu}L$까지 가능하였다. 양파 노균병균 검출용 PCR법은 육안상 병징이 나타나지 않을 때도 충분히 감염유무가 확인되었다.

Keywords

References

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