The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Five Previously Unreported Endophytic Fungi Isolated from the Leaves of Woody Plants in Korea

목본식물의 잎에서 분리된 5종의 미기록 내생균

  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Shim, Jae-Sung (Department of Biology Education, Korea National University of Education) ;
  • Kim, Ji-Su (Department of Biology Education, Korea National University of Education) ;
  • Choi, Hang-Seok (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 박혁 (한국교원대학교 생물교육과) ;
  • 심재성 (한국교원대학교 생물교육과) ;
  • 김지수 (한국교원대학교 생물교육과) ;
  • 최항석 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2017.11.17
  • Accepted : 2017.11.23
  • Published : 2017.12.01
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Abstract

The leaves of two woody plant species, Pinus densiflora and Aronia melanocarpa, were collected in Korea, and endophytic fungi were isolated from these surface-sterilized leaves. The fungal isolates were identified based on their morphological characteristics and the results of the phylogenetic analysis involving nucleotide sequences of the internal transcribed spacer region (ITS), including 5.8S rDNA, D1/D2 regions of 28S rDNA, and ${\beta}-tubulin$ genes. Pestalotia lawsoniae and Zasmidium fructicola were isolated from Pinus densiflora, and three species, Pestalotiopsis chamaeropis, Pestalotiopsis jesteri, and Stagonosporopsis cucurbitacearum were isolated from Aronia melanocarpa. To the best of our knowledge, these species have not been previously reported in Korea.

본 연구에서는 소나무(Pinus densiflora)와 아로니아(Aronia melanocarpa)의 잎을 채취하여 표면살균한 후 내생균을 분리하였다. 분리한 균주는 형태적 특징과 internal transcribed spacer (ITS) rDNA 지역과 28S rDNA 지역 그리고 ${\beta}-tubulin$ 유전자의 염기서열을 이용하여 계통분석을 통해 동정하였다. 소나무에서 분리한 두 종의 균주인 Pestalotia lawsoniae와 Zasmidium fructicola, 그리고 아로니아에서 분리한 세 종의 균주인 Pestalotiopsis chamaeropis, Pestalotiopsis jesteri, Stagonosporopsis cucurbitacearum는 국내 미기록 진균으로 보고하고자 한다.

Keywords

References

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